Tcf7 (NM_009331) Mouse Tagged ORF Clone
CAT#: MR226713
- TrueORF®
Tcf7 (Myc-DDK-tagged) - Mouse transcription factor 7, T-cell specific (Tcf7)
ORF Plasmid: tGFP
"NM_009331" in other vectors (6)
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CNY 2,400.00
CNY 3,990.00
Cited in 5 publications. |
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | AI465550; TCF-1; Tcf1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR226713 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTACAAAGAGACTGTCTACTCTGCCTTCAATCTGCTCATGCCCTACCCACCGGCCTCGGGAGCAGGGC AGCATCCGCAGCCTCAACCCCCGCTGCATAACAAGCCCGGCCAGCCTCCACATGGCGTCCCCCAGCTTTC TCCACTCTACGAACATTTCAGCAGTCCACACCCCACCCCTGCACCAGCGGACATCAGCCAGAAGCAAGGA GTTCACAGGCCGCTGCAGACCCCCGATCTCTCTGGATTTTATTCTCTGACCTCAGGCAGCATGGGACAGC TCCCCCATACTGTGAGCTGGCCCAGTCCTCCTCTCTACCCCCTGTCCCCTTCCTGCGGATATAGACAGCA CTTCCCTGCCCCCACTGCAGCCCCTGGCGCCCCCTATCCCAGGTTCACCCACCCATCCTTGATGCTGGGA TCTGGTGTACCTGGACACCCAGCAGCCATCCCTCACCCGGCCATTGTGCCCTCCTCAGGGAAGCAGGAGC TGCAGCCATATGATAGAAACCTGAAAACACAGGCAGAACCCAAGGCAGAGAAGGAGGCTAAGAAGCCAGT CATCAAGAAACCCCTCAATGCGTTCATGCTTTACATGAAGGAGATGAGAGCCAAGGTCATTGCTGAGTGC ACACTCAAGGAGAGCGCTGCCATCAACCAGATCCTGGGTCGCAGGTGGCATGCACTATCTCGAGAAGAGC AGGCCAAGTACTATGAACTGGCCCGCAAGGAAAGGCAGCTGCACATGCAGCTATACCCGGGCTGGTCCGC GCGGGATAACTACGGAAAGAAGAAGAGGCGGTCAAGGGAAAAACATCAAGAATCCACCACAGGAGGAAAA AGAAATGCATTCGGTACTTACCCGGAGAAGGCCGCTGCCCCAGCCCCGTTCCTTCCGATGACAGTGCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR226713 protein sequence
Red=Cloning site Green=Tags(s) MYKETVYSAFNLLMPYPPASGAGQHPQPQPPLHNKPGQPPHGVPQLSPLYEHFSSPHPTPAPADISQKQG VHRPLQTPDLSGFYSLTSGSMGQLPHTVSWPSPPLYPLSPSCGYRQHFPAPTAAPGAPYPRFTHPSLMLG SGVPGHPAAIPHPAIVPSSGKQELQPYDRNLKTQAEPKAEKEAKKPVIKKPLNAFMLYMKEMRAKVIAEC TLKESAAINQILGRRWHALSREEQAKYYELARKERQLHMQLYPGWSARDNYGKKKRRSREKHQESTTGGK RNAFGTYPEKAAAPAPFLPMTVL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_009331 |
ORF Size | 912 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_009331.1 |
RefSeq Size | 2238 bp |
RefSeq ORF | 912 bp |
Locus ID | 21414 |
UniProt ID | Q00417 |
MW | 33.6 kDa |
Gene Summary | This gene encodes a transcription factor which is a member of the T-cell specific transcription factor family. The encoded protein is distinct from the hepatic transcription factor, transcription factor 1, which is also referred to by the symbol Tcf1. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Sep 2015] |
Citations (5)
The use of this cDNA Clones has been cited in the following citations: |
---|
Developmental Relationships of Four Exhausted CD8+ T Cell Subsets Reveals Underlying Transcriptional and Epigenetic Landscape Control Mechanisms
,Beltra, JC;Manne, S;Abdel-Hakeem, MS;Kurachi, M;Giles, JR;Chen, Z;Casella, V;Ngiow, SF;Khan, O;Huang, YJ;Yan, P;Nzingha, K;Xu, W;Amaravadi, RK;Xu, X;Karakousis, GC;Mitchell, TC;Schuchter, LM;Huang, AC;Wherry, EJ;,
Immunity
,PubMed ID 32396847
[TCF7]
|
TCF-1-Centered Transcriptional Network Drives an Effector versus Exhausted CD8 T Cell-Fate Decision
,Chen, Z;Ji, Z;Ngiow, SF;Manne, S;Cai, Z;Huang, AC;Johnson, J;Staupe, RP;Bengsch, B;Xu, C;Yu, S;Kurachi, M;Herati, RS;Vella, LA;Baxter, AE;Wu, JE;Khan, O;Beltra, JC;Giles, JR;Stelekati, E;McLane, LM;Lau, CW;Yang, X;Berger, SL;Vahedi, G;Ji, H;Wherry, EJ;,
Immunity
,PubMed ID 31606264
[TCF7]
|
Lineage-Determining Transcription Factor TCF-1 Initiates the Epigenetic Identity of T Cells
,Johnson, JL;Georgakilas, G;Petrovic, J;Kurachi, M;Cai, S;Harly, C;Pear, WS;Bhandoola, A;Wherry, EJ;Vahedi, G;,
Immunity
,PubMed ID 29466756
[TCF7]
|
A Novel miR-24-TCF1 Axis in Modulating Effector T Cell Responses
,Cho, S;Wu, CJ;Nguyen, DT;Lin, LL;Chen, MC;Khan, AA;Yang, BH;Fu, W;Lu, LF;,
J. Immunol.
,PubMed ID 28404635
[TCF7]
|
TCF1 links GIPR signaling to the control of beta cell function and survival
,Campbell, JE;Ussher, JR;Mulvihill, EE;Kolic, J;Baggio, LL;Cao, X;Liu, Y;Lamont, BJ;Morii, T;Streutker, CJ;Tamarina, N;Philipson, LH;Wrana, JL;MacDonald, PE;Drucker, DJ;,
Nat. Med.
,PubMed ID 26642437
[TCF7]
|
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC209498 | Tcf7 (untagged) - Mouse transcription factor 7, T-cell specific (Tcf7), (10ug) |
CNY 3,600.00 |
|
MG226713 | Tcf7 (tGFP-tagged) - Mouse transcription factor 7 T-cell specific (Tcf7), (10ug) |
CNY 4,370.00 |
|
MR226713L1 | Lenti ORF clone of Tcf7 (Myc-DDK-tagged) - Mouse transcription factor 7, T-cell specific (Tcf7) |
CNY 4,800.00 |
|
MR226713L2 | Lenti ORF clone of Tcf7 (mGFP-tagged) - Mouse transcription factor 7, T-cell specific (Tcf7) |
CNY 4,800.00 |
|
MR226713L3 | Lenti ORF clone of Tcf7 (Myc-DDK-tagged) - Mouse transcription factor 7, T-cell specific (Tcf7) |
CNY 5,890.00 |
|
MR226713L4 | Lenti ORF clone of Tcf7 (mGFP-tagged) - Mouse transcription factor 7, T-cell specific (Tcf7) |
CNY 4,800.00 |