Med12 (NM_021521) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	230kDa; Mopa; OPA-1; Tnrc11; Trap230
Vector	pCMV6-Entry2
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR225918 representing NM_021521 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCTTTCGGGATCTTGAGCTATGAACACCGACCCCTGAAGCGGCTGCGGCTGGGGCCTCCCGATG TGTACCCTCAAGATCCCAAACAGAAGGAGGATGAACTGACGGCTTTGAATGTAAAACAAGGTTTCAATAA CCAGCCTGCTGTCTCTGGGGACGAACATGGCAGTGCCAAGAACGTCAACTTCAATCCTGCCAAGATCAGT TCCAACTTCAGCAGCATCATCGCAGAGAAGTTAAGGTGTAATACTCTCTCTGACACTGGTCGCAGGAAGT CCCTAATGAACCAGAAGGACAACTTCTGGCTGGTGACTGCAAGATCTCAGAGTGCTATTAACACCTGGTT CACTGACCTGGCTGGCACCAAACCACTCACACACCTAGCCAAAAAGGTCCCCATTTTCAGTAAAAAGGAA GAAGTATTTGGGTATTTGGCCAAATACACGGTGCCTGTGATGCGGGCTGCCTGGCTCATTAAGATGACTT GTGCCTACTATGCAGCAATGTCTGAGACTAAGGTTAAGAAGAAAAATACTGCTGACCCCTTCACTGAATG GACTCAAATCATCACAAAGTACTTGTGGGAGCAGCTACAGAAGATGGCTGAGTACTATCGGCCAGGGCCT GCAGGAAGTGGAGGCTGTGGTTCTACTATAGGGCCTTTGCCCCATGATGTAGAGATGGCCATCAGGCAGT GGGATTACAATGAGAAGCTCGCCTTGTTCATGTTTCAGGATGGAATGCTGGACAGACATGAGTTCCTGAC TTGGGTGCTTGAGTGTTTTGAGAAAATACGCCCTGGAGAAGATGAATTGCTTAAATTGCTGCTTCCCCTA CTGCTGCGATACTCAGGGGAGTTTGTTCAGTCTGCCTATCTGTCCCGCCGCCTTGCCTACTTCTGTACCC GGAGATTGGCTCTCCAGCTGGATGGTGTGAGCAGCCACTCATCTCATGTCATAGCTGCTCAGTCAACAAG TTCTCTGCCCACTACCCCAGCACCTCAGCCCCCAACTAGCAGTACACCCTCGACTCCCTTTAGTGACCTG CTTATGTGCCCTCAGCATCGTCCCCTGGTTTTTGGCCTCAGCTGTATCCTTCAGACCATCCTTCTGTGTT GCCCTAGTGCCCTAGTTTGGCACTACTCATTGACTGATAGCCGAATTAAGACCGGCTCACCACTTGACCA CCTGCCTATTGCTCCTTCCAACTTGCCCATGCCAGAGGGTAACAGTGCCTTTACTCAGCAGGTCCGTGCA AAATTACGAGAGATCGAACAACAGATCAAGGAGCGTGGACAAGCAGTTGAGGTTCGCTGGTCTTTTGATA AGTGCCAGGAAGCTACTGCAGGTTTCACCATTGGACGGGTGCTCCATACTTTAGAAGTGCTGGATAGCCA TAGTTTCGAGCGCTCTGACTTTAGTAACTCTCTTGACTCCCTTTGTAATCGAATCTTTGGATTGGGGCCT AGTAAGGATGGTCATGAGATTTCCTCTGATGATGATGCTGTGGTATCATTATTGTGTGAATGGGCTGTAA GCTGCAAACGCTCAGGTCGGCATCGTGCTATGGTAGTAGCCAAACTCCTAGAGAAGAGACAAGCAGAAAT TGAGGCTGAGCGCTGCGGAGAATCTGAAGCAGCTGATGAGAAGGGCTCCGTCGCCTCTGGTTCCCTTTCC GCTCCTAGTGCACCCATTTTCCAGGATGTCCTCCTGCAGTTTCTGGATACACAGGCTCCCATGCTGACTG ATCCCCGAAGTGAAAGTGAGCGAGTAGAATTCTTTAACTTGGTACTGCTCTTCTGTGAACTGATCCGACA TGATGTCTTCTCCCATAATATGTACACCTGCACTCTCATCTCTCGGGGGGATCTTGCATTTGGAGCCCCT GGTCCTCGGCCTCCCTCTCCCTTTGATGATCCTACAGATGATCCAGAGCGCAAGGAGGCTGAAGGCAGCA GCAGCAGCAAGCTAGAGGACCCAGGGCTCTCTGAATCTATGGACATCGACCCTAGTTCCAGTGTGCTTTT TGAGGACATGGAAAAGCCTGATTTCTCATTGTTCTCCCCTACTATGCCTTGTGAGGGGAAGGGAAGCCCA TCCCCTGAGAAACCAGATGTCGAAAAGGAAGTAAAACCCCCAGCCAAAGAGAAGATCGAGGGGACACTTG GGATTCTGTATGACCAGCCACGACATGTGCAGTATGCCACACACTTTCCAATCCCACAGGAGGAGTCATG CAGCCATGAGTGCAACCAGCGGTTGGTCGTACTGTTTGGGGTGGGGAAGCAGCGAGATGATGCCCGCCAT GCCATCAAGAAGATTACCAAGGATATCCTGAAGGTTCTGAATCGCAAGGGGACAGCAGAAACTGACCAGC TTGCTCCTATTGTGCCTCTGAATCCTGGAGACCTGACATTCTTAGGTGGGGAAGATGGGCAGAAGCGACG ACGCAACCGGCCCGAAGCCTTCCCCACTGCTGAAGATATTTTTGCTAAGTTCCAGCACCTTTCCCATTAT GACCAACACCAGGTCACGGCTCAGGTCTCCCGGAACGTTCTGGAGCAGATCACGAGCTTCGCCCTTGGCA TGTCGTACCACTTGCCTCTGGTGCAGCATGTGCAGTTCATCTTCGACCTCATGGAATATTCACTGAGCAT CAGTGGCCTCATCGACTTTGCCATCCAGTTACTGAATGAGCTGAGTGTGGTTGAGGCGGAGCTCCTTCTC AAATCGTCGGATCTGGTGGGCAGCTACACTACCAGCCTGTGCTTATGTATCGTGGCTGTCCTTCGACACT ATCATGCCTGCCTCATCCTCAATCAGGACCAGATGGCGCAAGTGTTTGAGGGGCTCTGTGGCGTGGTGAA ACATGGAATGAACCGTTCAGATGGCTCCTCTGCAGAGCGCTGTATCCTTGCTTATCTCTATGATCTGTAT ACCTCCTGTAGCCATTTAAAGAGCAAATTTGGGGAGCTCTTCAGTGACTTTTGCTCAAAAGTGAAGAACA CCATCTACTGTAACGTGGAGCCATCGGAATCCAATATGCGCTGGGCACCGGAGTTCATGATTGACACTCT GGAGAACCCTGCCGCTCACACTTTCACCTACACGGGGCTCGGCAAGAGTCTTAGTGAGAACCCTGCTAAC CGCTATAGCTTTGTGTGCAATGCTCTTATGCACGTCTGCGTGGGCCACCATGATCCTGATAGGGTAAATG ACATCGCCATCCTTTGTGCAGAGCTGACCGGCTATTGCAAGTCCTTGAGTGCTGAGTGGCTAGGAGTACT TAAGGCCTTGTGCTGCTCCTCGAACAATGGCACTTGTGGTTTCAATGATCTCCTGTGCAACGTAGACGTC AGTGATTTGTCTTTTCATGATTCCTTGGCTACTTTTGTTGCTATCCTCATCGCCCGTCAATGTTTGCTCC TAGAAGACCTGATTCGCTGTGCAGCCATCCCTTCACTCCTCAATGCTGCTTGTAGTGAGCAGGATTCTGA GCCAGGAGCCAGGCTTACTTGCCGAATCCTCCTCCACCTGTTCAAGACGCCACAACTCAATCCTTGCCAG TCTGATGGAAACAAACCTACTGTTGGAATCCGGTCCTCCTGTGACCGCCACCTGCTGGCTGCCTCCCAGA ACCGCATCGTGGATGGGGCTGTGTTTGCTGTTCTCAAGGCTGTGTTTGTACTCGGAGATGCGGAGCTAAA AGGTTCAGGCTTTACTGTGCCGGGAGGAACAGAAGAACTTCCAGAAGAGGAGGGAGGAGGTGGTAGTAGC GGTCGGAGACAGGGTGGCCGCAACATCTCTGTGGAGACAGCAAGTCTGGATGTCTATGCCAAGTACGTGC TGCGAAGCATCTGCCAACAGGAATGGGTAGGAGAACGTTGCCTTAAGTCACTGTGTGAGGATAGCAATGA TCTACAAGACCCAGTGTTGAGTAGTGCCCAGGCCCAGCGCCTCATGCAACTTATCTGCTACCCACATCGA CTGCTGGACAATGAGGATGGAGAAAACCCCCAGCGGCAGCGCATTAAACGTATTCTCAAGAATTTAGACC AATGGACCATGCGCCAGTCCTCTTTGGAGCTACAGTTGATGATCAAGCAGACCCCCAACACTGAGATGAA CTCTCTCTTGGAGAACATTGCCAAGGCCACAATCGAGGTTTTCCAACAGTCAGCAGAGACGGGGTCATCT TCTGGAAGTACAGCAAGCAACATGCCCAGCAGCAGCAAGACCAAACCTGTGCTCAGCTCTCTAGAGCGAT CTGGTGTATGGTTGGTGGCTCCTCTCATTGCCAAACTGCCCACTTCAGTCCAGGGCCATGTATTAAAAGC TGCTGGGGAGGAACTGGAAAAGGGTCAGCACCTGGGCTCTTCTTCCCGTAAAGAACGAGATCGACAGAAA CAGAAGAGCATGTCCCTGTTGAGCCAACAGCCTTTCTTATCACTGGTGCTAACATGTCTGAAAGGACAGG ATGAGCAGCGCGAGGGACTCCTGGCCTCCCTCCACAGCCAGGTGCACCAGATTGTGATTAATTGGCGAGA AAACCAGTACTTAGATGATTGCAAACCAAAGCAGCTAATGCATGAGGCACTCAAACTGCGGCTCAACCTG GTGGGGGGCATGTTTGACACTGTGCAGCGTAGTACCCAGCAGACTACAGAGTGGGCCCAGCTTCTCCTTG AGATCATCATCAGCGGCACTGTGGACATGCAGTCTAACAATGAGCTCTTCACTACTGTGTTGGACATGCT GAGCGTGCTTATCAACGGAACGTTGGCTGCAGACATGTCCAGTATCTCGCAAGGCAGCATGGAGGAAAAC AAGCGTGCATATATGAACCTGGTGAAGAAGCTTCAGAAGGACTTGGGGGAGCGCCAATCAGACAGTCTGG AGAAGGTTCACCAACTGTTGCCACTACCCAAGCAGAACCGAGATGTCATAACCTGTGAGCCACAGGGCTC CCTTATTGACACCAAGGGCAACAAGATTGCTGGCTTCGATTCCATCTTCAAGAAGGAGGGTCTACAAGTT TCTACCAAACAAAAGATCTCTCCCTGGGAGCTTTTTGAGGGCCTGAAGCCATCAACAGCACCACTGTCAT GGGCCTGGTTTGGCACAGTCCGAGTGGACCGCAGAGTGGCACGAGGGGAGGAGCAGCAGCGGCTGTTGCT CTATCATACCCACCTGAGGCCTCGACCCAGAGCCTATTACCTGGAACCACTACCTCTGCCCCCAGAAGAT GAGGAGCCACCAGCCCCTGCCCTACTAGAGCCTGAGAAAAAGGCTCCTGAGCCCCCCAAGACTGACAAAC CAGGGGCTGCTCCTCCGAGCACTGAGGAGCGCAAAAAGAAGTCTACCAAGGGCAAAAAACGCAGCCAGCC AGCCACCAAGAACGAGGACTATGGCATGGGGCCAGGTCGGAGTGGCCCCTATGGTGTGACAGTGCCTCCA GACCTTCTACACCATGCAAATCCTGGTTCTATATCCCACCTTAGCTACAGGCAAAGCTCCATGGGCCTGT ATACCCAAAACCAGCCACTACCTGCTGGTGGCCCTCGTGTGGATCCATACCGCCCCGTGCGATTACCAAT GCAAAAGCTGCCAACTCGACCAACTTATCCCGGTGTGCTGCCTACAACTATGTCTACTGTCATGGGCCTA GAACCCTCTTCTTATAAGACATCTGTATACCGGCAGCAGCAACCCACAGTGCCCCAGGGACAGCGCCTTC GCCAACAGCTCCAGGCAAAGATACAGAGTCAGGGGATGTTGGGACAGTCATCTGTCCATCAGATGACCCC TAGTTCTTCCTATGGTTTGCAGACTTCCCAGGGCTATACATCCTATGTTTCTCATGTGGGATTGCAGCAA CACACAGGCCCTGCAGGTACCATGGTGCCCCCCAGCTACTCCAGCCAGCCTTATCAGAGCACCCACCCTT CTACCAATCCTACTCTTGTAGATCCTACCCGCCACCTGCAACAGCGGCCCAGTGGCTATGTGCATCAGCA GGCCCCAACCTATGGGCATGGACTGACTTCCACTCAAAGGTTTTCACACCAGACACTGCAGCAGACACCC ATGATGGGTACCATGACTCCGTTGAGTGCCCAGGGTGTCCAGGCAGGCGTCCGTTCAACTTCCATCCTGC CTGAGCAGCAGCAACAACAACAGCAGCAGCAGCAACAGCAGCAGCAGCAACAGCAGCAGCAGCAACAACA ACAGCAGCAGCAGCAGCAGCAACAACAACAGCAGTACCATATCCGACAGCAACAGCAGCAGCAGCAGATG CTACGGCAACAGCAGCAACAACAGCAACAGCAGCAGCAGCAGCAACAGCAGCAGCAGCAACAACAACAGC AACAGCAGCAGCAGCAGCCACACCAGCAGCAGCAGCAGGCAGCTCCTCCCCAACCCCAGCCCCAGTCCCA GCCCCAGTTCCAGCGCCAGGGCCTGCAGCAGACCCAGCAGCAGCAACAGACAGCAGCTTTGGTCCGGCAA CTTCAACAACAGCTCTCTAATACCCAGCCACAGCCCAGCACCAACATATTTGGACGCTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR225918 representing NM_021521 Red=Cloning site Green=Tags(s) MAAFGILSYEHRPLKRLRLGPPDVYPQDPKQKEDELTALNVKQGFNNQPAVSGDEHGSAKNVNFNPAKIS SNFSSIIAEKLRCNTLSDTGRRKSLMNQKDNFWLVTARSQSAINTWFTDLAGTKPLTHLAKKVPIFSKKE EVFGYLAKYTVPVMRAAWLIKMTCAYYAAMSETKVKKKNTADPFTEWTQIITKYLWEQLQKMAEYYRPGP AGSGGCGSTIGPLPHDVEMAIRQWDYNEKLALFMFQDGMLDRHEFLTWVLECFEKIRPGEDELLKLLLPL LLRYSGEFVQSAYLSRRLAYFCTRRLALQLDGVSSHSSHVIAAQSTSSLPTTPAPQPPTSSTPSTPFSDL LMCPQHRPLVFGLSCILQTILLCCPSALVWHYSLTDSRIKTGSPLDHLPIAPSNLPMPEGNSAFTQQVRA KLREIEQQIKERGQAVEVRWSFDKCQEATAGFTIGRVLHTLEVLDSHSFERSDFSNSLDSLCNRIFGLGP SKDGHEISSDDDAVVSLLCEWAVSCKRSGRHRAMVVAKLLEKRQAEIEAERCGESEAADEKGSVASGSLS APSAPIFQDVLLQFLDTQAPMLTDPRSESERVEFFNLVLLFCELIRHDVFSHNMYTCTLISRGDLAFGAP GPRPPSPFDDPTDDPERKEAEGSSSSKLEDPGLSESMDIDPSSSVLFEDMEKPDFSLFSPTMPCEGKGSP SPEKPDVEKEVKPPAKEKIEGTLGILYDQPRHVQYATHFPIPQEESCSHECNQRLVVLFGVGKQRDDARH AIKKITKDILKVLNRKGTAETDQLAPIVPLNPGDLTFLGGEDGQKRRRNRPEAFPTAEDIFAKFQHLSHY DQHQVTAQVSRNVLEQITSFALGMSYHLPLVQHVQFIFDLMEYSLSISGLIDFAIQLLNELSVVEAELLL KSSDLVGSYTTSLCLCIVAVLRHYHACLILNQDQMAQVFEGLCGVVKHGMNRSDGSSAERCILAYLYDLY TSCSHLKSKFGELFSDFCSKVKNTIYCNVEPSESNMRWAPEFMIDTLENPAAHTFTYTGLGKSLSENPAN RYSFVCNALMHVCVGHHDPDRVNDIAILCAELTGYCKSLSAEWLGVLKALCCSSNNGTCGFNDLLCNVDV SDLSFHDSLATFVAILIARQCLLLEDLIRCAAIPSLLNAACSEQDSEPGARLTCRILLHLFKTPQLNPCQ SDGNKPTVGIRSSCDRHLLAASQNRIVDGAVFAVLKAVFVLGDAELKGSGFTVPGGTEELPEEEGGGGSS GRRQGGRNISVETASLDVYAKYVLRSICQQEWVGERCLKSLCEDSNDLQDPVLSSAQAQRLMQLICYPHR LLDNEDGENPQRQRIKRILKNLDQWTMRQSSLELQLMIKQTPNTEMNSLLENIAKATIEVFQQSAETGSS SGSTASNMPSSSKTKPVLSSLERSGVWLVAPLIAKLPTSVQGHVLKAAGEELEKGQHLGSSSRKERDRQK QKSMSLLSQQPFLSLVLTCLKGQDEQREGLLASLHSQVHQIVINWRENQYLDDCKPKQLMHEALKLRLNL VGGMFDTVQRSTQQTTEWAQLLLEIIISGTVDMQSNNELFTTVLDMLSVLINGTLAADMSSISQGSMEEN KRAYMNLVKKLQKDLGERQSDSLEKVHQLLPLPKQNRDVITCEPQGSLIDTKGNKIAGFDSIFKKEGLQV STKQKISPWELFEGLKPSTAPLSWAWFGTVRVDRRVARGEEQQRLLLYHTHLRPRPRAYYLEPLPLPPED EEPPAPALLEPEKKAPEPPKTDKPGAAPPSTEERKKKSTKGKKRSQPATKNEDYGMGPGRSGPYGVTVPP DLLHHANPGSISHLSYRQSSMGLYTQNQPLPAGGPRVDPYRPVRLPMQKLPTRPTYPGVLPTTMSTVMGL EPSSYKTSVYRQQQPTVPQGQRLRQQLQAKIQSQGMLGQSSVHQMTPSSSYGLQTSQGYTSYVSHVGLQQ HTGPAGTMVPPSYSSQPYQSTHPSTNPTLVDPTRHLQQRPSGYVHQQAPTYGHGLTSTQRFSHQTLQQTP MMGTMTPLSAQGVQAGVRSTSILPEQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQYHIRQQQQQQQM LRQQQQQQQQQQQQQQQQQQQQQQQQQQQPHQQQQQAAPPQPQPQSQPQFQRQGLQQTQQQQQTAALVRQ LQQQLSNTQPQPSTNIFGRY TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene
ACCN	NM_021521
ORF Size	6570 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_021521.2, NP_067496.2
RefSeq Size	8464 bp
RefSeq ORF	6573 bp
Locus ID	59024
UniProt ID	A2AGH6
MW	245 kDa
Gene Summary	Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway (By similarity).[UniProtKB/Swiss-Prot Function]

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