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Runx1t1 (NM_001111026) Mouse Tagged ORF Clone
CAT＃: MR225849
- TrueORF®
Runx1t1 (Myc-DDK-tagged) - Mouse runt-related transcription factor 1, translocated to, 1 (cyclin D-related) (Runx1t1), transcript variant 2
ORF Plasmid: tGFP
"NM_001111026" in other vectors (4)
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CNY 4,352.00
CNY 5,130.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Cbfa2t1h; ETO; MTG8 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR225849 representing NM_001111026
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGGGACTCTCCAGCCCTGTGCAGTATCGTACCGAGAAGCACTCCACAATGCCAGACTCACCTGTGG ATGTGAAGACGCAGTCTAGGCTGACTCCCCCAGCAATGCCACCTCCTCCAACCACTCAAGGAGCTCCAAG AACCAGCTCATTTACGCCAACGACATTAACGAATGGCACCAGCCATTCACCCACGGCCTTGAATGGCGCT CCCTCACCGCCTAATGGCTTTAGCAACGGGCCTTCTTCTTCCTCTTCCTCTTCTCTGGCTAATCAGCAGC TGCCCCCAGCCTGTGGTGCTAGGCAACTCAGCAAGCTGAAACGATTTCTTACTACCCTGCAGCAGTTTGG CAATGACATTTCACCAGAGATTGGAGAAAGAGTTCGCACCCTTGTACTGGGACTGGTGAACTCTACTTTG ACAATTGAAGAATTTCATTCCAAACTGCAAGAAGCTACTAACTTCCCACTGAGACCTTTTGTCATCCCGT TTTTGAAGGCCAACCTGCCCCTGCTCCAGCGTGAACTCCTCCACTGCGCAAGGCTAGCCAAACAGAACCC TGCCCAGTACCTCGCTCAGCACGAACAGCTGCTTCTGGATGCCAGCACCACCTCCCCTGTCGACTCCTCA GAGCTGCTTCTCGACGTGAACGAAAACGGGAAGAGGCGAACTCCAGACAGAACCAAAGAAAATGGCTTTG ACAGAGAGCCTTTGCACTCAGAACATCCAAGCAAGCGACCATGCACTATTAGCCCAGGCCAGCGGTACAG TCCAAATAATGGCTTATCCTACCAGCCAAATGGCCTGCCTCACCCCACCCCACCTCCACCTCAGCATTAC CGATTGGATGATATGGCCATTGCCCACCACTACAGGGACTCCTATCGACACCCCAGCCACAGGGACCTCA GGGACAGAAATAGACCTATGGGGTTACATGGCACACGTCAAGAAGAAATGATTGACCACAGACTAACAGA CAGAGAATGGGCAGAAGAGTGGAAACATCTCGATCATCTGTTAAACTGCATCATGGACATGGTGGAGAAG ACAAGACGATCCCTCACTGTACTAAGACGATGTCAGGAAGCGGACCGGGAAGAACTGAATTACTGGATAC GGCGGTACAGTGATGCTGAGGACTTAAAAAAGGGTGGCAGCAGTAGCAGCAGCCACTCCAGGCAGCAGAG TCCTGTCAATCCAGACCCAGTCGCATTAGATGCACATCGGGAATTCCTTCACAGGCCTGCGTCTGGATAC GTGCCAGAGGAGATCTGGAAGAAAGCTGAGGAAGCTGTGAATGAGGTGAAGCGACAGGCAATGACGGAGC TGCAAAAAGCCGTGTCTGAGGCAGAGAGGAAAGCCCATGACATGATCACCACGGAGCGAGCCAAGATGGA ACGCACGGTGGCTGAAGCGAAGAGGCAGGCAGCAGAAGATGCTCTAGCAGTCATCAACCAGCAGGAAGAC TCCAGTGAGAGTTGCTGGAACTGTGGCCGGAAAGCAAGTGAGACGTGCAGCGGCTGTAACACGGCCCGAT ACTGTGGCTCTTTTTGCCAGCATAAAGACTGGGAGAAGCATCACCACATCTGTGGACAGACCCTGCAGGC CCCACAGCAGGGAGACACGCCCGCTGTCAGCTCCTCAGTCACACCCAGCAGTGGGGCTGGGAGCCCAATG GACACACCACCAGCAGCCACTCCGAGGTCTACCACCCCGGGAACCCCCTCTACCATAGAGACGACGCCTC GC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR225849 representing NM_001111026
Red=Cloning site Green=Tags(s) MVGLSSPVQYRTEKHSTMPDSPVDVKTQSRLTPPAMPPPPTTQGAPRTSSFTPTTLTNGTSHSPTALNGA PSPPNGFSNGPSSSSSSSLANQQLPPACGARQLSKLKRFLTTLQQFGNDISPEIGERVRTLVLGLVNSTL TIEEFHSKLQEATNFPLRPFVIPFLKANLPLLQRELLHCARLAKQNPAQYLAQHEQLLLDASTTSPVDSS ELLLDVNENGKRRTPDRTKENGFDREPLHSEHPSKRPCTISPGQRYSPNNGLSYQPNGLPHPTPPPPQHY RLDDMAIAHHYRDSYRHPSHRDLRDRNRPMGLHGTRQEEMIDHRLTDREWAEEWKHLDHLLNCIMDMVEK TRRSLTVLRRCQEADREELNYWIRRYSDAEDLKKGGSSSSSHSRQQSPVNPDPVALDAHREFLHRPASGY VPEEIWKKAEEAVNEVKRQAMTELQKAVSEAERKAHDMITTERAKMERTVAEAKRQAAEDALAVINQQED SSESCWNCGRKASETCSGCNTARYCGSFCQHKDWEKHHHICGQTLQAPQQGDTPAVSSSVTPSSGAGSPM DTPPAATPRSTTPGTPSTIETTPR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001111026 |
| ORF Size | 1752 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001111026.2, NP_001104496.1 |
| RefSeq Size | 5930 bp |
| RefSeq ORF | 1755 bp |
| Locus ID | 12395 |
| MW | 65.5 kDa |
| Gene Summary | Transcriptional corepressor which facilitates transcriptional repression via its association with DNA-binding transcription factors and recruitment of other corepressors and histone-modifying enzymes. Can repress the expression of MMP7 in a ZBTB33-dependent manner. Can repress transactivation mediated by TCF12 (By similarity). Acts as a negative regulator of adipogenesis (PubMed:23527555).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC219265 | Runx1t1 (untagged) - Mouse runt-related transcription factor 1, translocated to, 1 (cyclin D-related) (Runx1t1), transcript variant 2, (10ug) |
CNY 7,410.00 |
|
| MG225849 | Runx1t1 (tGFP-tagged) - Mouse runt-related transcription factor 1; translocated to 1 (cyclin D-related) (Runx1t1) transcript variant 2, (10ug) |
CNY 5,610.00 |
|
| MR225849L3 | Lenti ORF clone of Runx1t1 (Myc-DDK-tagged) - Mouse runt-related transcription factor 1; translocated to, 1 (cyclin D-related) (Runx1t1), transcript variant 2 |
CNY 7,030.00 |
|
| MR225849L4 | Lenti ORF clone of Runx1t1 (mGFP-tagged) - Mouse runt-related transcription factor 1; translocated to, 1 (cyclin D-related) (Runx1t1), transcript variant 2 |
CNY 7,030.00 |
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