Cldn14 (NM_001165925) Mouse Tagged ORF Clone

CAT#: MR225403

  • TrueORF®

Cldn14 (Myc-DDK-tagged) - Mouse claudin 14 (Cldn14), transcript variant 2

ORF Plasmid: DDK tGFP



  "NM_001165925" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 1,416.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms AI851731
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR225403 representing NM_001165925.
Blue=ORF Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGCCAGCACAGCGGTCCAGCTCCTAGGCTTCCTGCTTAGCTTCCTGGGCATGGTGGGAACGCTCATC
ACCACCATTCTGCCACACTGGCGGAGGACGGCCCATGTGGGCACCAACATCCTGACTGCCGTGTCCTAC
CTGAAGGGACTGTGGATGGAATGTGTGTGGCACAGCACAGGCATCTACCAGTGTCAGATCTACCGCTCA
CTGCTGGCGCTGCCCCGGGATCTGCAGGCCGCCCGGGCGCTCATGGTCATCTCCTGCCTGTTGTCGGGC
ATGGCCTGCGCCTGCGCAGTAGTGGGCATGAAGTGCACACGCTGCGCCAAAGGCACACCCGCCAAGACC
ACCTTTGCAGTGCTGGGGGGCGCACTCTTCCTGCTGGCCGGCCTGCTGTGCATGGTGGCCGTGTCCTGG
ACCACGAATGACGTGGTGCAGAATTTTTATAACCCGCTGCTGCCCAGTGGCATGAAGTTTGAAATCGGC
CAGGCCCTGTACCTGGGCTTCATCTCCTCATCCCTGTCTCTCATCGGGGGCACCCTGCTCTGCTTATCC
TGCCAGGACGAGGCCCCCTACAGACCCTACCCGCCCCAGTCCAGGGCCGGAGCTACCACCACGGCTACC
GCCCCTGCCTACCGCCCACCAGCGGCCTACAAGGACAACCGTGCCCCCTCGGTGACCTCAGCCGCGCAC
AGTGGGTACAGGCTGAATGACTACGTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
>Peptide sequence encoded by MR225403
Blue=ORF Red=Cloning site Green=Tag(s)

MASTAVQLLGFLLSFLGMVGTLITTILPHWRRTAHVGTNILTAVSYLKGLWMECVWHSTGIYQCQIYRS
LLALPRDLQAARALMVISCLLSGMACACAVVGMKCTRCAKGTPAKTTFAVLGGALFLLAGLLCMVAVSW
TTNDVVQNFYNPLLPSGMKFEIGQALYLGFISSSLSLIGGTLLCLSCQDEAPYRPYPPQSRAGATTTAT
APAYRPPAAYKDNRAPSVTSAAHSGYRLNDYV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001165925
ORF Size 717 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq Size 1283 bp
RefSeq ORF 720 bp
Locus ID 56173
UniProt ID Q9Z0S3
MW 25.6 kDa
Gene Summary This gene encodes a member of the claudin family of tight junction proteins. The encoded protein is an integral membrane protein that may function in maintaining apical membrane polarization in tight junctions located between outer hair cells and supporting cells. Loss of function of this gene is associated with hearing problems. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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