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Cldn14 (NM_019500) Mouse Tagged ORF Clone
CAT＃: MR225401
- TrueORF®
Cldn14 (Myc-DDK-tagged) - Mouse claudin 14 (Cldn14), transcript variant 1
ORF Plasmid: tGFP
"NM_019500" in other vectors (4)
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CNY 1,416.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AI851731 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR225401 representing NM_019500
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAGCACAGCGGTCCAGCTCCTAGGCTTCCTGCTTAGCTTCCTGGGCATGGTAGGAACGCTCATCA CCACCATCCTGCCACACTGGCGGAGGACGGCCCATGTGGGCACCAACATCCTGACTGCCGTATCCTACCT GAAGGGACTGTGGATGGAATGTGTGTGGCACAGCACAGGCATCTACCAGTGTCAGATCTACCGCTCACTG CTGGCGCTGCCCCGGGACTTGCAGGCCGCCCGGGCGCTCATGGTCATCTCCTGCCTGTTGTCGGGCATGG CCTGCGCCTGCGCAGTAGTGGGCATGAAGTGCACACGCTGCGCCAAAGGCACACCCGCCAAGACCACCTT TGCAGTGCTGGGGGGCGCGCTCTTCCTGCTGGCCGGCCTGCTGTGCATGGTGGCCGTGTCCTGGACCACG AATGACGTGGTGCAGAATTTTTATAACCCGCTGCTGCCCAGTGGCATGAAGTTTGAAATCGGCCAGGCCC TGTACCTGGGCTTCATCTCCTCATCCCTGTCTCTCATCGGGGGCACCCTGCTCTGCTTATCCTGCCAGGA CGAGGCCCCCTACAGACCCTACCCGCCCCAGTCCAGGGCCGGAGCTACCACCACGGCTACCGCCCCTGCC TACCGCCCACCAGCGGCCTACAAGGACAACCGTGCCCCCTCGGTGACCTCAGCCGCGCACAGTGGGTACA GGCTGAATGACTACGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR225401 representing NM_019500
Red=Cloning site Green=Tags(s) MASTAVQLLGFLLSFLGMVGTLITTILPHWRRTAHVGTNILTAVSYLKGLWMECVWHSTGIYQCQIYRSL LALPRDLQAARALMVISCLLSGMACACAVVGMKCTRCAKGTPAKTTFAVLGGALFLLAGLLCMVAVSWTT NDVVQNFYNPLLPSGMKFEIGQALYLGFISSSLSLIGGTLLCLSCQDEAPYRPYPPQSRAGATTTATAPA YRPPAAYKDNRAPSVTSAAHSGYRLNDYV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_019500 |
| ORF Size | 717 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_019500.4, NP_062373.3 |
| RefSeq Size | 1475 bp |
| RefSeq ORF | 720 bp |
| Locus ID | 56173 |
| UniProt ID | Q9Z0S3 |
| MW | 26.1 kDa |
| Gene Summary | This gene encodes a member of the claudin family of tight junction proteins. The encoded protein is an integral membrane protein that may function in maintaining apical membrane polarization in tight junctions located between outer hair cells and supporting cells. Loss of function of this gene is associated with hearing problems. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Localization and Regulation of Claudin-14 in Experimental Models of Hypercalcemia
,Frische, S;Alexander, RT;Ferreira, P;Tan, R;Wang, W;Svenningsen, P;Skjødt, K;Dimke, H;,
American journal of physiology. Renal physiology
,PubMed ID 33283646
[CLDN14]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC210022 | Cldn14 (untagged) - Mouse claudin 14 (Cldn14), transcript variant 1, (10ug) |
CNY 3,990.00 |
|
| MG225401 | Cldn14 (tGFP-tagged) - Mouse claudin 14 (Cldn14) transcript variant 1, (10ug) |
CNY 2,850.00 |
|
| MR225401L3 | Lenti ORF clone of Cldn14 (Myc-DDK-tagged) - Mouse claudin 14 (Cldn14), transcript variant 1 |
CNY 4,750.00 |
|
| MR225401L4 | Lenti ORF clone of Cldn14 (mGFP-tagged) - Mouse claudin 14 (Cldn14), transcript variant 1 |
CNY 4,750.00 |
