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Mertk (NM_008587) Mouse Tagged ORF Clone
CAT＃: MR225392
- TrueORF®
Mertk (Myc-DDK-tagged) - Mouse c-mer proto-oncogene tyrosine kinase (Mertk)
ORF Plasmid: tGFP
"NM_008587" in other vectors (6)
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CNY 10,920.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Eyk; Mer; nmf12; Nyk |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR225392 representing NM_008587
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTTCTGGCCCCACTGCTACTGGGGCTGCTGCTGCTACCCGCGCTCTGGAGTGGAGGCACTGCCGAGA AGTGGGAAGAGACCGAGCTAGATCAGCTATTTTCAGGGCCTTTACCAGGGAGACTCCCAGTCAACCACAG GCCATTCTCTGCTCCTCACTCCAGCCGGGACCAGCTGCCACCACCCCAGACTGGAAGATCACATCCAGCA CACACAGCCGCTCCCCAGGTGACCTCCACAGCATCAAAGCTCCTACCTCCTGTTGCGTTTAATCACACCA TTGGACACATAGTACTGTCGGAACATAAAAATGTCAAATTTAATTGCTCCATCAATATTCCTAACACATA CCAAGAAACAGCTGGCATTTCATGGTGGAAAGATGGAAAGGAATTGCTCGGGGCACATCATTCAATCACA CAGTTTTATCCTGATGAGGAAGGGGTATCAATAATTGCATTGTTCAGCATAGCCAGTGTGCAGCGCTCAG ACAATGGGTCGTACTTCTGTAAGATGAAGGTGAACAATAGAGAGATTGTATCTGATCCCATATACGTGGA AGTTCAAGGACTCCCTTACTTTATTAAGCAGCCTGAGAGTGTGAATGTCACCAGAAACACAGCCTTCAAC CTCACCTGCCAGGCCGTGGGCCCTCCTGAGCCCGTCAATATCTTCTGGGTTCAAAATAGCAGCCGTGTTA ATGAAAAACCGGAAAGGTCCCCGTCTGTCCTAACCGTACCTGGTCTGACAGAGACAGCAGTCTTCAGCTG TGAGGCCCACAATGACAAAGGACTGACGGTGTCCAAGGGTGTACATATCAACATCAAAGTAATCCCCTCC CCGCCCACTGAAGTCCATATCCTCAACAGTACAGCACACAGCATCCTGGTCTCCTGGGTCCCTGGTTTTG ATGGCTACTCCCCACTTCAGAACTGCAGCATTCAGGTCAAGGAAGCTGACCGGCTGAGTAATGGCTCAGT CATGGTTTTTAATACCTCTGCTTCGCCACATCTGTATGAGATCCAGCAGCTGCAAGCCCTGGCTAATTAC AGCATCGCTGTGTCCTGTCGGAATGAGATTGGCTGGTCTGCAGTAAGCCCTTGGATTCTGGCCAGCACAA CAGAAGGAGCTCCATCTGTAGCACCTTTAAACATCACTGTGTTTCTGAACGAATCTAACAATATCCTGGA TATTAGATGGACGAAGCCTCCAATTAAGCGGCAGGATGGGGAACTGGTGGGCTACCGGATATCTCACGTG TGGGAAAGCGCAGGGACTTACAAAGAGCTTTCTGAAGAAGTCAGCCAGAATGGCAGCTGGGCTCAGATTC CTGTCCAAATCCACAATGCCACCTGCACAGTGAGAATCGCGGCCATTACTAAAGGGGGCATCGGGCCCTT CAGTGAGCCAGTGAATATCATCATTCCTGAACACAGTAAGGTAGATTACGCACCCTCGTCAACCCCAGCC CCTGGCAACACCGACTCTATGTTCATCATCCTCGGCTGCTTCTGTGGATTCATTTTAATCGGGTTAATTT TGTGTATTTCTCTGGCCCTCAGAAGGAGAGTCCAGGAAACAAAGTTTGGGGGAGCATTCTCTGAGGAGGA TTCCCAACTGGTCGTAAATTATAGAGCGAAGAAGTCCTTCTGCCGGCGAGCCATCGAGCTTACCTTGCAG AGCCTGGGAGTGAGCGAGGAGCTGCAGAATAAGCTGGAAGATGTTGTGATTGACAGAAACCTTCTGGTTC TCGGCAAAGTTCTGGGTGAAGGAGAGTTTGGGTCTGTAATGGAAGGAAATTTGAAGCAAGAAGATGGGAC TTCTCAGAAGGTGGCAGTGAAGACCATGAAGTTGGACAACTTTTCTCAACGGGAGATCGAGGAGTTTCTC AGCGAAGCAGCATGCATGAAAGACTTCAACCACCCAAATGTCATCCGACTTCTAGGCGTGTGTATAGAAC TGAGCTCTCAAGGCATCCCGAAGCCCATGGTGATTTTACCCTTCATGAAATACGGAGACCTCCACACCTT CCTGTTATATTCCCGATTAAACACAGGACCCAAGTACATTCACCTGCAGACACTACTGAAGTTCATGATG GACATTGCCCAGGGAATGGAGTATCTGAGCAACAGGAATTTTCTTCATAGGGATTTGGCAGCTCGAAACT GCATGTTGCGGGATGACATGACTGTCTGCGTGGCAGACTTTGGCCTCTCAAAGAAGATTTACAGTGGTGA TTATTACCGCCAAGGCCGCATTGCCAAAATGCCTGTGAAGTGGATCGCCATCGAGAGCCTGGCGGACCGA GTCTACACAAGCAAAAGTGACGTGTGGGCTTTTGGCGTGACCATGTGGGAAATAACAACACGGGGAATGA CTCCCTATCCCGGAGTTCAGAACCATGAGATGTACGACTACCTTCTCCACGGCCACAGGCTGAAGCAGCC TGAGGACTGCTTGGATGAACTGTATGACATCATGTACTCTTGCTGGAGTGCTGATCCCTTGGATCGACCC ACCTTCTCTGTGTTGAGGCTGCAGCTGGAAAAGCTCTCCGAGAGTTTGCCTGATGCGCAGGACAAAGAAT CCATCATCTACATCAATACCCAGTTGCTAGAGAGCTGCGAGGGCATAGCCAATGGGCCCTCACTCACGGG GCTAGACATGAACATTGACCCTGACTCCATCATTGCCTCTTGCACACCAGGCGCTGCCGTCAGCGTGGTC ACGGCAGAAGTTCACGAGAACAACCTTCGTGAGGAAAGATACATCTTGAATGGGGGCAATGAGGAATGGG AAGATGTGTCCTCCACTCCTTTTGCTGCAGTCACACCTGAAAAGGATGGTGTCTTACCGGAGGACAGACT CACCAAAAATGGCGTCTCCTGGTCTCACCATAGTACACTACCCTTGGGGAGCCCATCACCAGATGAACTT TTATTTGTAGATGACTCCTTGGAAGACTCTGAAGTTCTGATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR225392 representing NM_008587
Red=Cloning site Green=Tags(s) MVLAPLLLGLLLLPALWSGGTAEKWEETELDQLFSGPLPGRLPVNHRPFSAPHSSRDQLPPPQTGRSHPA HTAAPQVTSTASKLLPPVAFNHTIGHIVLSEHKNVKFNCSINIPNTYQETAGISWWKDGKELLGAHHSIT QFYPDEEGVSIIALFSIASVQRSDNGSYFCKMKVNNREIVSDPIYVEVQGLPYFIKQPESVNVTRNTAFN LTCQAVGPPEPVNIFWVQNSSRVNEKPERSPSVLTVPGLTETAVFSCEAHNDKGLTVSKGVHINIKVIPS PPTEVHILNSTAHSILVSWVPGFDGYSPLQNCSIQVKEADRLSNGSVMVFNTSASPHLYEIQQLQALANY SIAVSCRNEIGWSAVSPWILASTTEGAPSVAPLNITVFLNESNNILDIRWTKPPIKRQDGELVGYRISHV WESAGTYKELSEEVSQNGSWAQIPVQIHNATCTVRIAAITKGGIGPFSEPVNIIIPEHSKVDYAPSSTPA PGNTDSMFIILGCFCGFILIGLILCISLALRRRVQETKFGGAFSEEDSQLVVNYRAKKSFCRRAIELTLQ SLGVSEELQNKLEDVVIDRNLLVLGKVLGEGEFGSVMEGNLKQEDGTSQKVAVKTMKLDNFSQREIEEFL SEAACMKDFNHPNVIRLLGVCIELSSQGIPKPMVILPFMKYGDLHTFLLYSRLNTGPKYIHLQTLLKFMM DIAQGMEYLSNRNFLHRDLAARNCMLRDDMTVCVADFGLSKKIYSGDYYRQGRIAKMPVKWIAIESLADR VYTSKSDVWAFGVTMWEITTRGMTPYPGVQNHEMYDYLLHGHRLKQPEDCLDELYDIMYSCWSADPLDRP TFSVLRLQLEKLSESLPDAQDKESIIYINTQLLESCEGIANGPSLTGLDMNIDPDSIIASCTPGAAVSVV TAEVHENNLREERYILNGGNEEWEDVSSTPFAAVTPEKDGVLPEDRLTKNGVSWSHHSTLPLGSPSPDEL LFVDDSLEDSEVLM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008587 |
| ORF Size | 2982 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008587.2 |
| RefSeq Size | 3564 bp |
| RefSeq ORF | 2985 bp |
| Locus ID | 17289 |
| UniProt ID | Q60805 |
| MW | 110.6 kDa |
| Gene Summary | Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including LGALS3, TUB, TULP1 or GAS6. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222992 | Mertk (untagged) - Mouse c-mer proto-oncogene tyrosine kinase (Mertk), (10ug) |
CNY 10,928.00 |
|
| MG225392 | Mertk (tGFP-tagged) - Mouse c-mer proto-oncogene tyrosine kinase (Mertk), (10ug) |
CNY 12,520.00 |
|
| MR225392L1 | Lenti ORF clone of Mertk (Myc-DDK-tagged) - Mouse c-mer proto-oncogene tyrosine kinase (Mertk) |
CNY 11,780.00 |
|
| MR225392L2 | Lenti ORF clone of Mertk (mGFP-tagged) - Mouse c-mer proto-oncogene tyrosine kinase (Mertk) |
CNY 13,320.00 |
|
| MR225392L3 | Lenti ORF clone of Mertk (Myc-DDK-tagged) - Mouse c-mer proto-oncogene tyrosine kinase (Mertk) |
CNY 11,780.00 |
|
| MR225392L4 | Lenti ORF clone of Mertk (mGFP-tagged) - Mouse c-mer proto-oncogene tyrosine kinase (Mertk) |
CNY 13,320.00 |
