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Trpv3 (NM_145099) Mouse Tagged ORF Clone
CAT＃: MR225379
- TrueORF®
Trpv3 (Myc-DDK-tagged) - Mouse transient receptor potential cation channel, subfamily V, member 3 (Trpv3)
ORF Plasmid: tGFP
"NM_145099" in other vectors (4)
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CNY 8,688.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1110036I10Rik; AI644701; Nh; VRL3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR225379 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATGCCCACTCCAAGGAGATGGTGCCCCTCATGGGCAAAAGAACCACAGCACCTGGCGGGAACCCTG TTGTACTGACGGAGAAGAGGCCAGCAGATCTCACCCCCACCAAGAAGAGTGCACACTTCTTCCTGGAGAT AGAAGGATTTGAGCCCAACCCCACGGTCACCAAGACCTCTCCACCCATCTTCTCCAAGCCGATGGACTCC AACATCCGGCAGTGCCTCTCTGGCAACTGTGATGACATGGACTCTCCCCAGTCTCCTCAGGATGATGTGA CAGAGACCCCATCCAATCCCAACAGTCCGAGCGCAAACCTGGCCAAGGAAGAACAGAGGCAGAAGAAGAA GCGACTGAAGAAGCGCATTTTCACGGCTGTGTCTGAGGGATGCGTGGAGGAGCTGCGGGAACTCCTACAG GATCTGCAGGACCTCTGCAGGAGGCGCCGCGGCCTGGATGTGCCTGACTTCCTCATGCACAAGCTGACAG CCTCAGACACTGGGAAGACCTGCCTGATGAAGGCTTTGCTCAACATCAATCCCAACACCAAAGAGATCGT GCGGATTCTGCTTGCCTTCGCTGAGGAGAACGACATCCTGGACAGGTTCATCAACGCTGAGTACACGGAA GAGGCCTATGAAGGGCAGACAGCGCTGAACATCGCCATCGAGCGGCGCCAGGGAGACATCACAGCAGTGC TTATAGCAGCGGGTGCTGACGTCAATGCTCACGCCAAGGGGGTCTTCTTCAACCCCAAATACCAGCATGA AGGCTTCTATTTTGGCGAGACACCCCTGGCTTTGGCAGCGTGTACCAACCAGCCTGAGATTGTGCAGCTG CTGATGGAGAATGAGCAGACAGACATCGCTTCCCAGGATTCCCGGGGAAACAACATCCTGCACGCGCTGG TGACGGTGGCTGAGGACTTCAAGACTCAGAATGACTTCGTTAAGCGCATGTATGACATGATCCTGCTGAG GAGTGGCAACTGGGAGCTGGAGACCATGCGCAACAACGATGGGCTCACGCCACTGCAGCTGGCTGCCAAG ATGGGCAAGGCTGAGATCCTGAAGTACATCCTCAGCCGCGAGATCAAGGAGAAGCCTCTCCGGAGCTTGT CCAGGAAGTTCACGGACTGGGCGTATGGGCCTGTGTCATCCTCACTCTATGACCTCACCAATGTAGACAC AACGACGGATAACTCTGTGCTGGAAATCATCGTCTACAACACCAACATTGATAACCGACATGAGATGCTG ACCCTGGAGCCTCTGCATACGCTGCTACACACGAAATGGAAGAAATTTGCCAAGTACATGTTCTTCTTGT CCTTCTGCTTCTATTTCTTCTACAACATCACCCTGACCCTTGTCTCTTACTACCGTCCTCGGGAAGATGA GGATCTCCCACACCCCTTGGCCCTGACACACAAAATGAGTTGGCTTCAGCTCCTAGGGAGGATGTTTGTC CTCATCTGGGCCACATGCATCTCTGTGAAAGAAGGCATTGCCATTTTCCTGCTGAGACCCTCCGATCTTC AGTCCATCCTGTCAGATGCCTGGTTTCACTTTGTCTTTTTTGTCCAAGCTGTACTTGTGATACTGTCTGT ATTCTTGTACTTGTTTGCCTACAAAGAATACCTCGCCTGCCTCGTGCTGGCCATGGCCCTGGGCTGGGCG AACATGCTCTACTACACGAGAGGCTTCCAGTCTATGGGCATGTACAGCGTCATGATCCAGAAGGTCATTT TGCATGATGTCCTCAAGTTCTTGTTTGTTTACATCCTGTTCTTACTTGGATTTGGAGTAGCGCTGGCCTC ACTGATTGAGAAGTGCTCCAAGGACAAAAAGGACTGCAGTTCCTATGGCAGCTTCAGCGACGCGGTGCTG GAGCTCTTCAAGCTCACCATAGGCCTGGGCGACCTGAACATCCAGCAGAACTCCACCTACCCCATCCTCT TTCTCTTCCTACTCATCACCTATGTCATCCTCACCTTCGTCCTCCTCCTCAACATGCTCATTGCCCTGAT GGGGGAGACGGTGGAGAACGTCTCCAAAGAAAGTGAGCGGATCTGGCGCTTGCAGAGAGCCAGGACCATC TTGGAGTTTGAGAAAATGTTACCAGAATGGCTGAGAAGCAGATTCCGCATGGGCGAGCTGTGCAAAGTAG CAGATGAGGACTTCCGGCTGTGTCTGCGGATCAACGAGGTGAAGTGGACGGAATGGAAAACACACGTGTC CTTCCTTAATGAAGACCCGGGACCCATAAGACGGACAGATTTAAACAAGATTCAAGATTCTTCCAGGAGC AATAGCAAAACCACCCTCTATGCGTTTGATGAATTAGATGAATTCCCAGAAACGTCGGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR225379 protein sequence
Red=Cloning site Green=Tags(s) MNAHSKEMVPLMGKRTTAPGGNPVVLTEKRPADLTPTKKSAHFFLEIEGFEPNPTVTKTSPPIFSKPMDS NIRQCLSGNCDDMDSPQSPQDDVTETPSNPNSPSANLAKEEQRQKKKRLKKRIFTAVSEGCVEELRELLQ DLQDLCRRRRGLDVPDFLMHKLTASDTGKTCLMKALLNINPNTKEIVRILLAFAEENDILDRFINAEYTE EAYEGQTALNIAIERRQGDITAVLIAAGADVNAHAKGVFFNPKYQHEGFYFGETPLALAACTNQPEIVQL LMENEQTDIASQDSRGNNILHALVTVAEDFKTQNDFVKRMYDMILLRSGNWELETMRNNDGLTPLQLAAK MGKAEILKYILSREIKEKPLRSLSRKFTDWAYGPVSSSLYDLTNVDTTTDNSVLEIIVYNTNIDNRHEML TLEPLHTLLHTKWKKFAKYMFFLSFCFYFFYNITLTLVSYYRPREDEDLPHPLALTHKMSWLQLLGRMFV LIWATCISVKEGIAIFLLRPSDLQSILSDAWFHFVFFVQAVLVILSVFLYLFAYKEYLACLVLAMALGWA NMLYYTRGFQSMGMYSVMIQKVILHDVLKFLFVYILFLLGFGVALASLIEKCSKDKKDCSSYGSFSDAVL ELFKLTIGLGDLNIQQNSTYPILFLFLLITYVILTFVLLLNMLIALMGETVENVSKESERIWRLQRARTI LEFEKMLPEWLRSRFRMGELCKVADEDFRLCLRINEVKWTEWKTHVSFLNEDPGPIRRTDLNKIQDSSRS NSKTTLYAFDELDEFPETSV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_145099 |
| ORF Size | 2373 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_145099.1, NM_145099.2, NP_659567.2 |
| RefSeq Size | 2440 bp |
| RefSeq ORF | 2376 bp |
| Locus ID | 246788 |
| UniProt ID | Q8K424 |
| MW | 90.6 kDa |
| Gene Summary | Putative receptor-activated non-selective calcium permeant cation channel. It is activated by innocuous (warm) temperatures and shows an increased response at noxious temperatures greater than 39 degrees Celsius. Activation exhibits an outward rectification. May associate with TRPV1 and may modulate its activity. Is a negative regulator of hair growth and cycling: TRPV3-coupled signaling suppresses keratinocyte proliferation in hair follicles and induces apoptosis and premature hair follicle regression (catagen) (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221667 | Trpv3 (untagged) - Mouse transient receptor potential cation channel, subfamily V, member 3 (Trpv3), (10ug) |
CNY 8,704.00 |
|
| MG225379 | Trpv3 (tGFP-tagged) - Mouse transient receptor potential cation channel subfamily V member 3 (Trpv3), (10ug) |
CNY 8,650.00 |
|
| MR225379L3 | Lenti ORF clone of Trpv3 (Myc-DDK-tagged) - Mouse transient receptor potential cation channel, subfamily V, member 3 (Trpv3) |
CNY 9,790.00 |
|
| MR225379L4 | Lenti ORF clone of Trpv3 (mGFP-tagged) - Mouse transient receptor potential cation channel, subfamily V, member 3 (Trpv3) |
CNY 9,790.00 |
