United States
Germany
Japan
United Kingdom
China
Hmbs (NM_013551) Mouse Tagged ORF Clone
CAT＃: MR223896
- TrueORF®
Hmbs (Myc-DDK-tagged) - Mouse hydroxymethylbilane synthase (Hmbs), transcript variant 1
ORF Plasmid: tGFP
"NM_013551" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 3,610.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | PBGD; T25658; Ups; Uros1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR223896 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCGGTAACGGCGGCGCGGCCACAACCGCGGAAGAAAACGGCTCAAAGATGAGGGTGATTCGAGTGG GCACCCGTAAGAGCCAGCTGGCTCGCATACAGACCGACACTGTGGTGGCGATGCTGAAAGCCTTGTACCC TGGCATACAGTTTGAAATCATTGCTATGTCCACCACGGGAGACAAGATTCTTGATACTGCACTCTCTAAG ATTGGAGAGAAGAGCCTGTTTACCAAGGAGCTAGAAAACGCCCTGGAAAAAAACGAAGTGGACCTGGTCG TTCACTCCCTGAAGGATGTGCCTACCATACTACCTCCTGGCTTTACTATTGGAGCCATCTGCAAACGGGA AAACCCTTGTGATGCTGTTGTCTTTCACCCAAAGTTTATTGGAAAGACCCTGGAAACCTTGCCAGAGAAA AGTGCCGTGGGAACCAGCTCTCTGAGGAGAGTGGCTCAGCTACAGAGAAAGTTCCCCCACCTGGAATTCA AGAGTATTCGGGGAAACCTCAACACCCGCCTCCGGAAGCTGGATGAGCTGCAGGAATTCAGTGCCATCGT CCTGGCTGTGGCTGGCCTACAGCGCATGGGCTGGCAGAACCGGGTGGGCCAGATTTTGCACCCAGAAGAA TGCATGTATGCTGTGGGTCAGGGAGCCCTAGCCGTGGAAGTCCGAGCCAAGGACCAGGATATCTTGGACC TAGTGAGTGTGTTGCACGATCCTGAAACTCTGCTTCGCTGCATTGCTGAAAGGGCTTTTCTGAGGCACCT GGAAGGAGGCTGCAGCGTGCCCGTAGCAGTGCATACAGTGATGAAAGATGGGCAACTGTACCTGACTGGT GGAGTCTGGAGTCTAGATGGCTCAGATAGCATGCAAGAGACTATGCAGGCCACCATCCAGGTCCCTGTTC AGCAAGAAGATGGTCCAGAAGATGACCCACAACTGGTTGGAATCACTGCCCGTAACATTCCAAGAGGAGC CCAGCTAGCTGCTGAGAACCTGGGCATCAGCCTGGCCAGCTTGCTGCTCAACAAAGGAGCCAAGAACATC CTGGATGTTGCACGGCAGCTTAATGATGTGCGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR223896 protein sequence
Red=Cloning site Green=Tags(s) MSGNGGAATTAEENGSKMRVIRVGTRKSQLARIQTDTVVAMLKALYPGIQFEIIAMSTTGDKILDTALSK IGEKSLFTKELENALEKNEVDLVVHSLKDVPTILPPGFTIGAICKRENPCDAVVFHPKFIGKTLETLPEK SAVGTSSLRRVAQLQRKFPHLEFKSIRGNLNTRLRKLDELQEFSAIVLAVAGLQRMGWQNRVGQILHPEE CMYAVGQGALAVEVRAKDQDILDLVSVLHDPETLLRCIAERAFLRHLEGGCSVPVAVHTVMKDGQLYLTG GVWSLDGSDSMQETMQATIQVPVQQEDGPEDDPQLVGITARNIPRGAQLAAENLGISLASLLLNKGAKNI LDVARQLNDVR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_013551 |
| ORF Size | 1083 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_013551.2, NP_038579.2 |
| RefSeq Size | 1611 bp |
| RefSeq ORF | 1086 bp |
| Locus ID | 15288 |
| UniProt ID | P22907 |
| MW | 39.3 kDa |
| Gene Summary | Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200084 | Hmbs (untagged) - Mouse hydroxymethylbilane synthase (Hmbs), transcript variant 1, (10ug) |
CNY 2,000.00 |
|
| MG223896 | Hmbs (tGFP-tagged) - Mouse hydroxymethylbilane synthase (Hmbs) transcript variant 1, (10ug) |
CNY 3,990.00 |
|
| MR223896L3 | Lenti ORF clone of Hmbs (Myc-DDK-tagged) - Mouse hydroxymethylbilane synthase (Hmbs), transcript variant 1 |
CNY 5,510.00 |
|
| MR223896L4 | Lenti ORF clone of Hmbs (mGFP-tagged) - Mouse hydroxymethylbilane synthase (Hmbs), transcript variant 1 |
CNY 5,510.00 |
