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Whrn (NM_028640) Mouse Tagged ORF Clone
CAT＃: MR223836
- TrueORF®
Whrn (Myc-DDK-tagged) - Mouse whirlin (Whrn), transcript variant 1
ORF Plasmid: tGFP
"NM_028640" in other vectors (4)
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CNY 6,648.00
CNY 7,130.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1110035G07Rik; AW122018; AW742671; C430046P22Rik; Dfnb3; Dfnb31; Ush2; Ush2d; w; wi |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR223836 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) ATGAACGCACAGCTGGACGGCCTGTCGGTGAGCTCGTCCTCCACCGGCTCGCTGGGCTCGGCGGCGGCGG CGGCGGGCGGCGGCGGGGGCGCGGGGTTGCGGCTGCTGTCTGCCAACGTGCGCCAGCTGCACCAAGCGCT CACGGCGCTGCTGAGCGAGCCCGAGCGGGAGCAGTTCACTCACTGCCTCAACGCGTACCACGCGCGCCGC AACGTCTTCGACCTGGTGCGCACCCTGCGCGTCCTGCTGGACAGCCCGGTCAAGCGGCGTCTGCTGCCCA TGCTGCGTCTGGTCATCCCGCGCTCCGACCAGCTGCTCTTCGACCAGTACACGGCCGAGGGCCTCTACCT GCCAGCCACCACCCCCTACAGGCAGCCCGCCTGGGCCGCTCCCGACGGCGCGGGGCCCGGGGAGGTGCGA CTCGTGAGCCTGCGGCGCGCCAAGGCCCACGAGGGCTTGGGCTTCAGCATCCGCGGGGGCTCGGAACACG GCGTGGGCATCTACGTGTCTCTAGTGGAGCCGGGCTCCCTGGCAGAGAAGGAAGGGTTGCGGGTCGGGGA CCAGATTCTGCGCGTCAACGATAAATCTCTAGCCCGGGTGACCCACGCGGAGGCTGTCAAGGCTCTCAAA GGCTCCAAGAAGCTGGTGCTGTCTGTATACTCAGCTGGGCGTATCCCAGGGGGCTATGTGACCAACCACA TCTACACCTGGGTGGACCCACAGGGTCGAAGCACATCCCCTCCCTCCAGCCTGCCCCAGCCCCATGGCAG CACCCTGAGACAGCGTGAAGATGACCGAAGGAGTACCCTCCACCTCCTGCAGAGTGGAGATGAGAAAAAG GTGAACCTGGTGTTGGGGGACGGCCGGTCCTTGGGCCTCACGATCCGAGGTGGAGCAGAGTACGGCCTTG GCATTTACATCACTGGTGTGGACCCAGGCTCTGAAGCAGAGAGCAGCGGCCTCAAGGTTGGAGACCAGAT TCTGGAGGTGAATGGGCGGAGCTTTCTCAACATCCTGCATGATGAGGCAGTGAAGCTGCTCAAGTCATCC CGGCACCTCATCCTGACGGTGAAGGACGTCGGAAGGCTGCCCCACGCACGTACCACCGTGGACCAGACCA AGTGGATCGCCAGTTCCCGGATCGGGGAAAGCGTCGCCAACTCAGCAGGGTTTCCAGGGGACCACACAGA AGAAGGGACAAGCAAGCCAGGATTTTACAAGGGCCCAGCCGGCTCCCAGGTGACCCTGAGCAGCCTGGGG AATCAGACTCGTGCACTGCTGGACGACCAAGCCAGGCACCTGCTGACAGAGCAGGAGCGCGCCACCATGA TGTACTACCTGGCCCAGTACCGTGGTGGCACCATCTCTGTGGAGGCCATGGTCATGGCACTGTTTGAGTT GCTCAACACACACGCCAAGTTCTCACTCCTGTCTGAGGTGAGAAGCATCATCTCACCCCAAGACCTGGAC CGCTTTGACCACCTGGTGCTGAGGCGCGAGATCGAGTCTATGAAGGCGAGGCAGCCCCCCGGCCCCGGGG TTGGAGACACGTATTCCATGGTTTCCTACAGCGACACAGGCTCATCCACAGGCAGCCACGGCACCTCTAC AACCGTCAGCTCGGCCAGGAATACATTAGACCTGGAAGGAACCGGTGAGACCACCCAGGGCAGCACCAAC GCTCTCCCGGATGTGTCTGTGGATGATGTCAAATCCCCCTCAGAAGATCTGCCTGGTATCAAGCCACCTC CTCCCCCACCACCCCTGGCTCAAGGTCATGACCGCTTGCTTGGCCAGCCACGGAAGCCAGGGAGGGAGGA CCCCGCACCTCTGTCTTCTGCTGCCCACTCAGGCATTGTCTTCTCAGCACCACGGAACCGCAGCCCGCCG CCAGGGACTGCTCCCACTCCGGGACCTTCCTCAGCACAGGACTCTCCCTCCTCCCCCATTTATGCCTCCA TCTCCCATGCCAACCCCAGTTCCAGAAAGCCGCTGGACACCCACCTGGCCCTGGTTAACCAGCACCCCAT CGGCCCCTTCCCTCGAGTCCAGTCCCCACCTCACCTGAAGAGCCCTCCTGCAGAGACCCCAGGAGCTGGG GCCTGCCTTCCACCACCATCACCCTCTGAACACCCTGACGCCGTGGGTGCAAACCAGCACTTTGTTCTGG TGGAGGTGCACCGTCCGGACAGTGATCCTGATGTGAATGAAGTGCGGGCTCTGCCCCAGACTCGCACAGC CTCCACACTCTCTCAGCTCTCAGACAGTGGGCAGACCCTGAGCGAGGACAGTGGCGTGGATGCCGGGGAG ACGGAGGCCAGCACCTCAGGCCGAGGCAGACAGACAGCATCCGCTAAGAACAAGAATGGCAAGGAGCAGC CCCGGACGGAGAGGACCGCGGAGGGTGCCAACAAACCTCCCGGCCTGCTGGAGCCAACGTCCACCCTGGT CCGTGTGAGGAAAAGTGCAGCCACACTGGGCATCGCCATTGAGGGCGGTGCCAACACACGCCAGCCTCTG CCCAGGATCGTCACAATTCAGCGAGGAGGTTCTGCCCATAACTGTGGACAGCTCAAGGTGGGCCACGTAA TTCTGGAAGTGAATGGGCAGACACTTCGGGGTAAGGAGCACAAGGAGGCCGCCCAAATCATCGCTGAGGC CTTCAAGACCAAGGAGAGAGACTACATCGACTTTCTGGTCACTGAGTTCAACGTGATGCTC >MR223836 protein sequence
Red=Cloning site Green=Tags(s) MNAQLDGLSVSSSSTGSLGSAAAAAGGGGGAGLRLLSANVRQLHQALTALLSEPEREQFTHCLNAYHARR NVFDLVRTLRVLLDSPVKRRLLPMLRLVIPRSDQLLFDQYTAEGLYLPATTPYRQPAWAAPDGAGPGEVR LVSLRRAKAHEGLGFSIRGGSEHGVGIYVSLVEPGSLAEKEGLRVGDQILRVNDKSLARVTHAEAVKALK GSKKLVLSVYSAGRIPGGYVTNHIYTWVDPQGRSTSPPSSLPQPHGSTLRQREDDRRSTLHLLQSGDEKK VNLVLGDGRSLGLTIRGGAEYGLGIYITGVDPGSEAESSGLKVGDQILEVNGRSFLNILHDEAVKLLKSS RHLILTVKDVGRLPHARTTVDQTKWIASSRIGESVANSAGFPGDHTEEGTSKPGFYKGPAGSQVTLSSLG NQTRALLDDQARHLLTEQERATMMYYLAQYRGGTISVEAMVMALFELLNTHAKFSLLSEVRSIISPQDLD RFDHLVLRREIESMKARQPPGPGVGDTYSMVSYSDTGSSTGSHGTSTTVSSARNTLDLEGTGETTQGSTN ALPDVSVDDVKSPSEDLPGIKPPPPPPPLAQGHDRLLGQPRKPGREDPAPLSSAAHSGIVFSAPRNRSPP PGTAPTPGPSSAQDSPSSPIYASISHANPSSRKPLDTHLALVNQHPIGPFPRVQSPPHLKSPPAETPGAG ACLPPPSPSEHPDAVGANQHFVLVEVHRPDSDPDVNEVRALPQTRTASTLSQLSDSGQTLSEDSGVDAGE TEASTSGRGRQTASAKNKNGKEQPRTERTAEGANKPPGLLEPTSTLVRVRKSAATLGIAIEGGANTRQPL PRIVTIQRGGSAHNCGQLKVGHVILEVNGQTLRGKEHKEAAQIIAEAFKTKERDYIDFLVTEFNVML |
| Restriction Sites | SgfI-RsrII Cloning Scheme for this gene |
| ACCN | NM_028640 |
| ORF Size | 2724 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_028640.1, NM_028640.2, NP_082916.1 |
| RefSeq Size | 4056 bp |
| RefSeq ORF | 2724 bp |
| Locus ID | 73750 |
| UniProt ID | Q80VW5 |
| MW | 96.6 kDa |
| Gene Summary | This gene encodes a protein required for elongation and actin polymerization in the hair cell stereocilia. The encoded protein is localized to the cytoplasm and co-localizes with the growing end of actin filaments. Mutations in this gene have been linked to deafness. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2013] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222534 | Whrn (untagged) - Mouse whirlin (Whrn), transcript variant 1, (10ug) |
CNY 11,690.00 |
|
| MG223836 | Whrn (tGFP-tagged) - Mouse whirlin (Whrn) transcript variant 1, (10ug) |
CNY 7,890.00 |
|
| MR223836L3 | Lenti ORF clone of Whrn (Myc-DDK-tagged) - Mouse whirlin (Whrn), transcript variant 1 |
CNY 9,030.00 |
|
| MR223836L4 | Lenti ORF clone of Whrn (mGFP-tagged) - Mouse whirlin (Whrn), transcript variant 1 |
CNY 9,030.00 |
