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Sost (NM_024449) Mouse Tagged ORF Clone
CAT＃: MR222588
- TrueORF®
Sost (Myc-DDK-tagged) - Mouse sclerostin (Sost)
ORF Plasmid: tGFP
"NM_024449" in other vectors (4)
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CNY 2,081.00
| Cited in 3 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 5430411E23Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR222588 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGCCCTCACTAGCCCCGTGCCTCATCTGCCTACTTGTGCACGCTGCCTTCTGTGCTGTGGAGGGCC AGGGGTGGCAAGCCTTCAGGAATGATGCCACAGAGGTCATCCCAGGGCTTGGAGAGTACCCCGAGCCTCC TCCTGAGAACAACCAGACCATGAACCGGGCGGAGAATGGAGGCAGACCTCCCCACCATCCCTATGACGCC AAAGATGTGTCCGAGTACAGCTGCCGCGAGCTGCACTACACCCGCTTCCTGACAGACGGCCCATGCCGCA GCGCCAAGCCGGTCACCGAGTTGGTGTGCTCCGGCCAGTGCGGCCCCGCGCGGCTGCTGCCCAACGCCAT CGGGCGCGTGAAGTGGTGGCGCCCGAACGGACCGGATTTCCGCTGCATCCCGGATCGCTACCGCGCGCAG CGGGTGCAGCTGCTGTGCCCCGGGGGCGCGGCGCCGCGCTCGCGCAAGGTGCGTCTGGTGGCCTCGTGCA AGTGCAAGCGCCTCACCCGCTTCCACAACCAGTCGGAGCTCAAGGACTTCGGGCCGGAGACCGCGCGGCC GCAGAAGGGTCGCAAGCCGCGGCCCGGCGCCCGGGGAGCCAAAGCCAACCAGGCGGAGCTGGAGAACGCC TAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR222588 protein sequence
Red=Cloning site Green=Tags(s) MQPSLAPCLICLLVHAAFCAVEGQGWQAFRNDATEVIPGLGEYPEPPPENNQTMNRAENGGRPPHHPYDA KDVSEYSCRELHYTRFLTDGPCRSAKPVTELVCSGQCGPARLLPNAIGRVKWWRPNGPDFRCIPDRYRAQ RVQLLCPGGAAPRSRKVRLVASCKCKRLTRFHNQSELKDFGPETARPQKGRKPRPGARGAKANQAELENA Y TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_024449 |
| ORF Size | 636 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_024449.6 |
| RefSeq Size | 2066 bp |
| RefSeq ORF | 636 bp |
| Locus ID | 74499 |
| UniProt ID | Q99P68 |
| MW | 23.5 kDa |
| Gene Summary | Negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation.[UniProtKB/Swiss-Prot Function] |
Citations (3)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Disparate bone anabolic cues activate bone formation by regulating the rapid lysosomal degradation of sclerostin protein
,null,
eLife
,PubMed ID 33779549
[Sost]
|
|
Anti-USAG-1 therapy for tooth regeneration through enhanced BMP signaling
,Murashima-Suginami, A;Kiso, H;Tokita, Y;Mihara, E;Nambu, Y;Uozumi, R;Tabata, Y;Bessho, K;Takagi, J;Sugai, M;Takahashi, K;,
Science advances
,PubMed ID 33579703
[SOST]
|
|
Identification of Sclerostin as a Putative New Myokine Involved in the Muscle-to-Bone Crosstalk
,Magarò, MS;Bertacchini, J;Florio, F;Zavatti, M;Potì, F;Cavani, F;Amore, E;De Santis, I;Bevilacqua, A;Reggiani Bonetti, L;Torricelli, P;Maurel, DB;Biressi, S;Palumbo, C;,
Biomedicines
,PubMed ID 33445754
[SOST]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC211534 | Sost (untagged) - Mouse sclerostin (Sost), (10ug) |
CNY 2,400.00 |
|
| MG222588 | Sost (tGFP-tagged) - Mouse sclerostin (Sost), (10ug) |
CNY 2,850.00 |
|
| MR222588L3 | Lenti ORF clone of Sost (Myc-DDK-tagged) - Mouse sclerostin (Sost) |
CNY 4,750.00 |
|
| MR222588L4 | Lenti ORF clone of Sost (mGFP-tagged) - Mouse sclerostin (Sost) |
CNY 4,750.00 |
