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Celf4 (NM_133195) Mouse Tagged ORF Clone
CAT＃: MR220694
- TrueORF®
Celf4 (Myc-DDK-tagged) - Mouse CUGBP, Elav-like family member 4 (Celf4), transcript variant 4
ORF Plasmid: tGFP
"NM_133195" in other vectors (5)
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CNY 4,850.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | A230070D14Rik; Brul4; BRUNOL-4; Brunol4; C130060B05Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR220694 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTATATAAAGATGGCCACGTTAGCAAACGGACAGGCTGACAACGCGAGCCTCAGTACCAACGGGCTAG GCAGCAGCCCGGGCAGCGCCGGGCATATGAACGGATTAAGCCACAGCCCGGGGAACCCGTCGACCATTCC CATGAAGGACCACGATGCCATCAAGCTGTTCATTGGGCAGATCCCCCGAAACCTGGATGAGAAGGACCTC AAGCCCCTCTTCGAGGAGTTCGGCAAGATCTACGAGCTTACGGTTCTGAAGGACAGGTTCACAGGCATGC ACAAAGGCTGCGCTTTCCTCACCTACTGCGAGCGTGAGTCAGCGCTGAAGGCCCAGAGCGCGCTGCACGA GCAGAAGACCCTGCCCGGGATGAACCGGCCGATCCAGGTGAAGCCTGCGGACAGCGAGAGCCGAGGAGGT AGTAGCTGCCTGCGCCAGCCCCCTTCACATAGAAAACTCTTCGTGGGTATGCTCAACAAGCAACAATCTG AGGACGACGTGCGCCGCCTCTTCGAGGCCTTCGGGAACATCGAGGAGTGCACTATCCTGCGCGGGCCGGA CGGCAACAGCAAGGGGTGCGCCTTTGTGAAGTACTCCTCCCATGCCGAGGCACAAGCCGCCATCAACGCT CTACATGGCAGCCAGACCATGCCTGGAGCCTCCTCCAGCCTGGTGGTCAAGTTTGCAGACACTGACAAGG AGCGCACAATGCGACGGATGCAGCAGATGGCTGGCCAGATGGGCATGTTCAACCCCATGGCCATCCCCTT CGGAGCCTATGGCGCCTATGCTCAGGCACTGATGCAGCAGCAAGCAGCCCTCATGGCATCGGTCGCGCAA GGAGGCTACCTGAATCCCATGGCTGCCTTCGCTGCCGCCCAAATGCAGCAGATGGCGGCCCTCAACATGA ATGGCCTGGCAGCCGCACCTATGACCCCAACCTCAGGTGGCAGCACCCCTCCAGGCATCACTGCACCAGC TGTGCCTAGCATCCCATCCCCCATTGGGGTGAACGGCTTCACGGGCCTCCCCCCTCAGGCCAATGGGCAG CCTGCTGCGGAAGCTGTGTTTGCCAATGGCATTCACCCCTACCCAGCACAGAGCCCCACCGCAGCCGACC CCCTGCAGCAGGCCTACGCTGGAGTGCAGCAGTATGCAGGACCTGCCTACCCTGCTGCCTATGGTCAGAT TAGCCAGGCCTTTCCTCAGCCACCGCCAATGATTCCCCAGCAACAGAGAGAAGGGCCCGAGGGCTGTAAC CTGCTCATCTACCATCTGCCCCAGGAGTTTGGGGACGCTGAGCTGATGCAGATGTTCCTCCCTTTCGGCT TCGTGAGCTTCGACAACCCGGCCAGCGCACAGACCGCCATCCAGGCCATGAACGGCTTCCAGATAGGCAT GAAGAGGCTCAAGGTGCAGCTGAAGCGGCCCAAAGACGCCAATCGCCCGTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR220694 protein sequence
Red=Cloning site Green=Tags(s) MYIKMATLANGQADNASLSTNGLGSSPGSAGHMNGLSHSPGNPSTIPMKDHDAIKLFIGQIPRNLDEKDL KPLFEEFGKIYELTVLKDRFTGMHKGCAFLTYCERESALKAQSALHEQKTLPGMNRPIQVKPADSESRGG SSCLRQPPSHRKLFVGMLNKQQSEDDVRRLFEAFGNIEECTILRGPDGNSKGCAFVKYSSHAEAQAAINA LHGSQTMPGASSSLVVKFADTDKERTMRRMQQMAGQMGMFNPMAIPFGAYGAYAQALMQQQAALMASVAQ GGYLNPMAAFAAAQMQQMAALNMNGLAAAPMTPTSGGSTPPGITAPAVPSIPSPIGVNGFTGLPPQANGQ PAAEAVFANGIHPYPAQSPTAADPLQQAYAGVQQYAGPAYPAAYGQISQAFPQPPPMIPQQQREGPEGCN LLIYHLPQEFGDAELMQMFLPFGFVSFDNPASAQTAIQAMNGFQIGMKRLKVQLKRPKDANRPY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_133195 |
| ORF Size | 1452 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_133195.4 |
| RefSeq Size | 4121 bp |
| RefSeq ORF | 1455 bp |
| Locus ID | 108013 |
| UniProt ID | Q7TSY6 |
| MW | 51.8 kDa |
| Gene Summary | RNA-binding protein implicated in the regulation of pre-mRNA alternative splicing. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition. Promotes exclusion of both the smooth muscle (SM) and non-muscle (NM) exons in actinin pre-mRNAs. Activates the splicing of MAPT/Tau exon 10. Binds to muscle-specific splicing enhancer (MSE) intronic sites flanking the alternative exon 5 of TNNT2 pre-mRNA (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC216700 | Celf4 (untagged) - Mouse CUGBP, Elav-like family member 4 (Celf4), transcript variant 4, (10ug) |
CNY 6,270.00 |
|
| MG207757 | Celf4 (tGFP-tagged) - Mouse bruno-like 4, RNA binding protein (Drosophila) (Brunol4) |
CNY 5,230.00 |
|
| MG220694 | Celf4 (tGFP-tagged) - Mouse CUGBP Elav-like family member 4 (Celf4) transcript variant 4, (10ug) |
CNY 5,320.00 |
|
| MR220694L3 | Lenti ORF clone of Celf4 (Myc-DDK-tagged) - Mouse CUGBP, Elav-like family member 4 (Celf4), transcript variant 4 |
CNY 6,750.00 |
|
| MR220694L4 | Lenti ORF clone of Celf4 (mGFP-tagged) - Mouse CUGBP, Elav-like family member 4 (Celf4), transcript variant 4 |
CNY 6,750.00 |
