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Cdc25b (NM_023117) Mouse Tagged ORF Clone
CAT＃: MR218723
- TrueORF®
Cdc25b (Myc-DDK-tagged) - Mouse cell division cycle 25 homolog B (S. pombe) (Cdc25b), transcript variant 1
ORF Plasmid: tGFP
"NM_023117" in other vectors (4)
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CNY 5,700.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AI604853 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR218723 representing NM_023117
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGTACCCCTGCAGAAGTCTGCGCCGGGTTCAGCTCTCAGTCCTGCCCGCGTGCTGGGTGGCATTC AGCGGCCGCGCCACCTCTCGGTCTTTGAGTTTGAGTCTGATGGCTTCCTGGGGTCTCCGGAGCCTACAGC TTCCTCCTCTCCGGTTACCACTCTTACACAGACCATGCACAACCTCGCTGGGCTCGGCAGTGAGCCTCCA AAAGCTCAAGTAGGAAGCCTGTCGTTCCAGAACAGGCTGGCAGACCTATCCCTGTCCAGGCGCACCTCTG AGTGCTCCCTGTCATCTGAGTCCTCAGAATCTTCGGATGCAGGTCTGTGCATGGACTCCCCCAGCCCTGT GGACCCGCAGATGGCAGAGCGCACGTTTGAACAGGCCATTCAGGCAGCCAGTCGGGTCATTCAAAATGAG CAGTTTACCATAAAACGCTTCCGATCCTTACCAGTGAGGCTGCTGGAACACAGTCCGGTGCTGCAGAGCA TCACCAACTCCCGAGCACTGGACAGCTGGAGGAAAACTGAAGCAGGCTACCGAGCCGCCGCCAATAGTCC TGGGGAGGACAAAGAGAATGATGGATATATCTTCAAGATGCCACAGGAGCTCCCTCATTCCAGCTCTGCC CAAGCTTTGGCAGAATGGGTCAGCCGCAGACAGGCCTTTACCCAGAGGCCCAGCTCAGCCCCTGACTTGA TGTGTCTTACCACTGAGTGGAAGATGGAAGTAGAGGAGCTGAGCCCGGTGGCACAGTCTTCTTCCTTGAC TCCTGTCGAAAGGGCTTCTGAAGAAGATGACGGATTTGTGGACATCCTGGAGAGTGATTTAAAGGATGAC GAGAAGGTCCCCGCGGGCATGGAGAACCTCATTAGTGCCCCACTGGTCAAAAAGCTGGATAAGGAAGAGG AACAGGATCTCATCATGTTCAGCAAGTGCCAGAGGCTCTTCCGCTCCCCATCCATGCCATGCAGTGTGAT CCGACCCATCCTCAAGAGGCTAGAGCGGCCCCAGGACCGGGATGTGCCTGTCCAGAGCAAGCGCAGGAAA AGTGTGACACCCCTGGAAGAGCAGCAGCTTGAAGAACCTAAGGCCCGTGTCTTTCGCTCAAAGTCGCTGT GTCATGAGATTGAGAACATCCTGGATAGTGACCACCGTGGACTGATCGGAGATTACTCTAAGGCCTTCCT CCTGCAGACCGTGGATGGCAAACACCAAGACCTTAAGTACATCTCACCAGAAACTATGGTGGCCCTGTTA ACAGGCAAGTTCAGCAACATCGTGGAGAAATTTGTCATTGTGGACTGCAGATACCCCTATGAGTATGAAG GCGGGCATATCAAGAATGCTGTGAACCTGCCCCTGGAACGGGATGCTGAGACCTTTCTGCTGCAGCGTCC CATCATGCCTTGTAGCCTGGACAAGAGAATCATCCTCATTTTCCACTGTGAATTCTCGTCTGAGCGTGGA CCACGAATGTGCCGCTTCATCAGGGAACGGGACCGTGCAGCTAACGACTACCCCAGCCTGTACTACCCGG AGATGTACATCCTCAAAGGCGGCTACAAGGAGTTCTTCCCACAGCATCCGAACTTTTGTGAGCCCCAGGA CTACCGACCCATGAACCACGAGGCTTTCAGGGATGAGCTGAGGAACTTTCGCCTTAAGACTCGCAGCTGG GCTGGGGAACGGAGCAGGAGGGAACTTTGTAGCAGGCTGCAAGACCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR218723 representing NM_023117
Red=Cloning site Green=Tags(s) MEVPLQKSAPGSALSPARVLGGIQRPRHLSVFEFESDGFLGSPEPTASSSPVTTLTQTMHNLAGLGSEPP KAQVGSLSFQNRLADLSLSRRTSECSLSSESSESSDAGLCMDSPSPVDPQMAERTFEQAIQAASRVIQNE QFTIKRFRSLPVRLLEHSPVLQSITNSRALDSWRKTEAGYRAAANSPGEDKENDGYIFKMPQELPHSSSA QALAEWVSRRQAFTQRPSSAPDLMCLTTEWKMEVEELSPVAQSSSLTPVERASEEDDGFVDILESDLKDD EKVPAGMENLISAPLVKKLDKEEEQDLIMFSKCQRLFRSPSMPCSVIRPILKRLERPQDRDVPVQSKRRK SVTPLEEQQLEEPKARVFRSKSLCHEIENILDSDHRGLIGDYSKAFLLQTVDGKHQDLKYISPETMVALL TGKFSNIVEKFVIVDCRYPYEYEGGHIKNAVNLPLERDAETFLLQRPIMPCSLDKRIILIFHCEFSSERG PRMCRFIRERDRAANDYPSLYYPEMYILKGGYKEFFPQHPNFCEPQDYRPMNHEAFRDELRNFRLKTRSW AGERSRRELCSRLQDQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_023117 |
| ORF Size | 1728 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_023117.4, NP_075606.1 |
| RefSeq Size | 3139 bp |
| RefSeq ORF | 1731 bp |
| Locus ID | 12531 |
| UniProt ID | P30306 |
| MW | 65.9 kDa |
| Gene Summary | Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC219153 | Cdc25b (untagged) - Mouse cell division cycle 25 homolog B (S. pombe) (Cdc25b), transcript variant 1, (10ug) |
CNY 7,320.00 |
|
| MG218723 | Cdc25b (tGFP-tagged) - Mouse cell division cycle 25 homolog B (S. pombe) (Cdc25b) transcript variant 1, (10ug) |
CNY 6,270.00 |
|
| MR218723L3 | Lenti ORF clone of Cdc25b (Myc-DDK-tagged) - Mouse cell division cycle 25 homolog B (S. pombe) (Cdc25b), transcript variant 1 |
CNY 7,600.00 |
|
| MR218723L4 | Lenti ORF clone of Cdc25b (mGFP-tagged) - Mouse cell division cycle 25 homolog B (S. pombe) (Cdc25b), transcript variant 1 |
CNY 7,600.00 |
