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Kmt5b (NM_001167886) Mouse Tagged ORF Clone
CAT＃: MR217786
- TrueORF®
Suv420h1 (Myc-DDK-tagged) - Mouse suppressor of variegation 4-20 homolog 1 (Drosophila) (Suv420h1), transcript variant 4
ORF Plasmid: tGFP
"NM_001167886" in other vectors (4)
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CNY 8,550.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AA117471; C630029K18Rik; Suv4-20h1; Suv420h1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR217786 representing NM_001167886
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGTGGTTGGGAGACTCCAAGAACATGGTGGTGAATGGCAGGAGAAATGGAGGCAAGTTGTCTAATG ACCATCAGCAGAATCAATCAAAATTACAGCAGCACTCGGGCAAGGACACCCTGAAGACCGGCAGAAACGC CGTTGAGAGGCGGTCCAGCAGATGTCATGGTAACTCGGGATTTGAAGGGCAGAGCCGCTATGTGCCGTCC TCTGGAATGTCCGCCAAGGAGCTCTGTGAGAACGATGACTTAGCAACCAGTTTGGTTCTTGATCCCTACT TAGGTTTTCAGACACACAAAATGAACACTAGATTTCGGCCTATAAAAGGAAGGCAAGAAGAGCTAAAGGA AGTAATTGAACGCTTTAAGAAAGATGAACACTTAGAGAAAGCTTTCAAATGTTTGACTTCTGGGGAATGG GCACGGCATTATTTTCTCAACAAAAACAAAATGCAGGAGAAATTATTCAAGGAACATGTCTTTATTTACT TGCGGATGTTTGCAACTGACAGTGGATTTGAAATACTGCCTTGTAATAGATATTCTTCAGAACAAAATGG AGCCAAGATAGTTGCAACAAAAGAGTGGAAACGAAATGACAAAATAGAATTACTGGTGGGTTGTATTGCC GAACTTTCAGAAATTGAGGAGAACATGCTACTTAGACACGGAGAAAACGACTTCAGTGTCATGTATTCCA CAAGGAAAAATTGTGCTCAACTCTGGCTCGGTCCTGCTGCATTTATAAATCATGATTGCAGACCTAACTG TAAGTTTGTGTCAACTGGTCGAGATACAGCATGCGTTAAGGCTCTGAGAGATATTGAACCTGGAGAAGAA ATTTCTTGTTACTATGGAGATGGCTTTTTTGGAGAAAATAATGAGTTCTGCGAATGTTATACTTGTGAAA GACGGGGAACTGGTGCTTTTAAATCACGAGTAGGACTGCCTGCGCCTGCTCCTGTTATCAATAGCAAATA CGGACTTAGAGAAACAGATAAACGCTTAAATAGGCTTAAAAAGTTAGGTGACAGCAGCAAAAACTCAGAC AGTCAGTCTGTCAGCTCTAACACAGATGCAGACACCACTCAGGAAAAAGACAATGCAACTTCTAATCGAA AATCTTCAGTTGGTGTGAAAAAGAGCAGCAAGAGTCGAGCTCTGACAAGGCCGTCCATGCCGAGAGTCCC GGCTGCTTCCAACTCTACCTCACCCAAGCTAGTGCACACCAACAATCCCCGGGTACCAAAGAAACTGAGA AAGCCGGCAAAGCCTTTACTCTCCAAGATCAGACTGCGGAATCACTGCAAGCGGCTGGACCAGAAGAGCG CATCCCGCAAGCTCGAGATGGGGAGCTTAGTGCTTAAGGAGCCCAAAGTCGTGCTATATAAAAATTTGCC AATTAAGAAAGAAAGGGAGCCAGAGGGACCAGCCCATGCTGCAGTGGGGAGTGGGTGCTTGACTAGACAT GCTGCGAGAGAACACAGGCAGAATCATGGGAGAGGTGCTCATTCGCAGGGCGACAGTTTGCCCTGCACCT ACACAACCCGGCGCTCTTTGAGGACAAGGACAGGTCTGAAGGAGACCACTGACATCAAGCTTGAACCAAG TCCCTTGGATGGCTATAAAAATGGTATACTGGAACCTTGCCCAGACAGTGGCCAGCAGCCAACCCCAGAG GTGCTGGAAGAACTGGCTCCTGAGACTGCACACAGGGAGGAAGCATCCCAGGAGTGTCCCAAGAACGACT CCTGCCTGTCACGAAAGAAATTTCGACAAGTGAAACCTGTGAAACACTTAGCAAAGACCGAGGACTGCAG TCCAGAGCACAGCTTCCCTGGGAAAGACGGGCTGCCAGATTTGCCAGGGTCTCATCCTGATCAAGGTGAG CCCAGTGGCACAGTCAGGGTGCCCGTGAGCCACACGGACTCTGCTCCCTCACCGGTTGGCTGCTCTGTTG TCGCACCCGACAGCTTCACAAAAGACAGCTTCAGAACTGCACAAAGTAAAAAGAAGCGGCGGGTCACCAG GTACGATGCACAGCTGATCCTGGAGAACAGCTCTGGAATCCCCAAGCTGACGCTTCGCAGGCGGCACGAC AGCAGCAGCAAGACAAACGACCATGAGAGTGACGGCGTGAACTCCTCCAAGATCAGCATCAAACTCAGCA AGGACCACGACAGTGACAGCAACCTCTATGTTGCCAAGCTCAGTAACGGGGTCAGCGCAGGGCCGGGCAG CAGCTCCACCAAGCTCAAGATCCAGCTCAAGCGGGATGAGGAGAGCAGGGGGCCATGTGCAGAGGGCCTG CACGAGAACGGGGTGTGCTGCAGCGACCCCCTCTCCCTGCTCGAGTCCCAGATGGAGGTGGACGACTACA GTCAATATGAGGAGGACAGCACAGATGAATCCTCATCTTCTGAGGGGGAGGAGGAGGAGGAGGACTGCGA GGATGACTTCGATGATGACTTCATTCCTCTTCCTCCGGCAAAGCGGCTGAGGCTAATTGTTGGTAAAGAC TCCATAGATATTGACATTTCTTCAAGGAGAAGAGAAGATCAGTCTCTAAGACTGAACGCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR217786 representing NM_001167886
Red=Cloning site Green=Tags(s) MKWLGDSKNMVVNGRRNGGKLSNDHQQNQSKLQQHSGKDTLKTGRNAVERRSSRCHGNSGFEGQSRYVPS SGMSAKELCENDDLATSLVLDPYLGFQTHKMNTRFRPIKGRQEELKEVIERFKKDEHLEKAFKCLTSGEW ARHYFLNKNKMQEKLFKEHVFIYLRMFATDSGFEILPCNRYSSEQNGAKIVATKEWKRNDKIELLVGCIA ELSEIEENMLLRHGENDFSVMYSTRKNCAQLWLGPAAFINHDCRPNCKFVSTGRDTACVKALRDIEPGEE ISCYYGDGFFGENNEFCECYTCERRGTGAFKSRVGLPAPAPVINSKYGLRETDKRLNRLKKLGDSSKNSD SQSVSSNTDADTTQEKDNATSNRKSSVGVKKSSKSRALTRPSMPRVPAASNSTSPKLVHTNNPRVPKKLR KPAKPLLSKIRLRNHCKRLDQKSASRKLEMGSLVLKEPKVVLYKNLPIKKEREPEGPAHAAVGSGCLTRH AAREHRQNHGRGAHSQGDSLPCTYTTRRSLRTRTGLKETTDIKLEPSPLDGYKNGILEPCPDSGQQPTPE VLEELAPETAHREEASQECPKNDSCLSRKKFRQVKPVKHLAKTEDCSPEHSFPGKDGLPDLPGSHPDQGE PSGTVRVPVSHTDSAPSPVGCSVVAPDSFTKDSFRTAQSKKKRRVTRYDAQLILENSSGIPKLTLRRRHD SSSKTNDHESDGVNSSKISIKLSKDHDSDSNLYVAKLSNGVSAGPGSSSTKLKIQLKRDEESRGPCAEGL HENGVCCSDPLSLLESQMEVDDYSQYEEDSTDESSSSEGEEEEEDCEDDFDDDFIPLPPAKRLRLIVGKD SIDIDISSRRREDQSLRLNA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001167886 |
| ORF Size | 2580 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001167886.1, NP_001161358.1 |
| RefSeq Size | 6002 bp |
| RefSeq ORF | 2583 bp |
| Locus ID | 225888 |
| UniProt ID | Q3U8K7 |
| MW | 96.5 kDa |
| Gene Summary | Histone methyltransferase that specifically trimethylates 'Lys-20' of histone H4. H4 'Lys-20' trimethylation represents a specific tag for epigenetic transcriptional repression. Mainly functions in pericentric heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin in these regions. KMT5B is targeted to histone H3 via its interaction with RB1 family proteins (RB1, RBL1 and RBL2). Plays a role in myogenesis by regulating the expression of target genes, such as EID3.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222190 | Suv420h1 (untagged) - Mouse suppressor of variegation 4-20 homolog 1 (Drosophila) (Suv420h1), transcript variant 4, (10ug) |
CNY 10,930.00 |
|
| MG217786 | Suv420h1 (tGFP-tagged) - Mouse suppressor of variegation 4-20 homolog 1 (Drosophila) (Suv420h1) transcript variant 4, (10ug) |
CNY 9,410.00 |
|
| MR217786L3 | Lenti ORF clone of Suv420h1 (Myc-DDK-tagged) - Mouse suppressor of variegation 4-20 homolog 1 (Drosophila) (Suv420h1), transcript variant 4 |
CNY 10,450.00 |
|
| MR217786L4 | Lenti ORF clone of Suv420h1 (mGFP-tagged) - Mouse suppressor of variegation 4-20 homolog 1 (Drosophila) (Suv420h1), transcript variant 4 |
CNY 10,450.00 |
