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Arhgap17 (NM_001122642) Mouse Tagged ORF Clone
CAT＃: MR216395
- TrueORF®
Arhgap17 (Myc-DDK-tagged) - Mouse Rho GTPase activating protein 17 (Arhgap17), transcript variant 4
ORF Plasmid: tGFP
"NM_001122642" in other vectors (4)
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CNY 7,410.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 5730403H17Rik; Nadrin; Nadrin2; Rich1; Wbp15 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR216395 representing NM_001122642
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGAAGCAATTCAACCGCATGAAGCAGCTGGCCAATCAGACTGTCGGCAGAGCTGAGAAGACCGAAG TCCTCAGTGAAGATCTGCTACAGATTGAACGTCGCCTGGACACTGTGCGTTCAATGTGCCACCATTCACA TAAGCGTTTGATAGCCTGCTTCCAAGGTCAGCATGGCACTGATGCCGAGAGGAGACATAAAAAGCTTCCT CTGACAGCTCTTGCCCAGAACATGCAGGAGGCTTCAGCCCAGCTGGAAGAGTCTCTCTTGGGGAAGATGC TGGAGACCTGTGGGGACGCTGAGAACCAGCTGGCTCTTGAACTCTCACAACATGAAGTCTTTGTGGAGAA GGAGATCATGGACCCTCTCTACGGCATAGCAGAGGTGGAGATTCCCAATATCCAGAAGCAAAGGAAGCAG CTTGCTAGATTGGTGTTGGACTGGGATTCAGTCAGAGCGAGATGGAACCAAGCACACAAGTCTTCAGGAA CCAACTTTCAGGGGCTTCCATCCAAAATAGATACCCTAAAGGAAGAGATGGATGAAGCTGGGAATAAAGT TGAACAGTGCAAGGATCAACTTGCAGCAGACATGTACAACTTCATGGCCAAAGAAGGGGAGTATGGCAAG TTCTTCGTGACGTTATTAGAAGCCCAAGCAGATTACCATAGAAAAGCATTAGCAGTCTTAGAAAAGGCCC TTCCCGAAATGCGCGCCCATCAAGATAAGTGGGCAGAGAAGCCAGCCTTCGGCACACCTCTGGAGGAACA CCTGAAGAGGAGTGGGCGTGAGATCGCCCTGCCTATTGAGGCCTGTGTCATGTTGTTGCTGGAGACTGGC ATGAAAGAGGAGGGCCTTTTTCGGATTGGAGCTGGAGCCTCCAAGTTGAAGAAGCTGAAAGCTGCTCTCG ACTGCTCCACATCGCATCTGGATGAATTCTATTCTGATCCCCATGCTGTCGCAGGTGCTTTAAAGTCCTA TCTGCGGGAGTTGCCTGAGCCCTTAATGACTTTCAGTCTGTATGAAGAATGGACACAAGTTGCAAGTGTG CAGGATCAAGACAAAAAACTTCAATATTTATGGACAACATGTCAGAAGTTGCCGCCACAAAATTTTGTTA ACTTTAGGTATTTAATCAAGTTCCTTGCAAAGCTTGCCCAGACCAGTGACGTTAATAAAATGACTCCTAG CAACATAGCCATTGTCCTGGGCCCTAACCTCCTGTGGGCCAAACAAGAAGGAACGCTGGCTGAAATAGCG GCTGCCACATCGGTCCACGTGGTTGCAGTGATTGAGCCCATCATCCAGCATGCAGATTGGTTCTTCCCTG GAGAGGTAGAATTCAATGTATCAGAAGCATTTGTGCCACTTGCTACCCCGAATTCTAATCACTCATCCCA TACTGGAAATGACTCTGACTCGGGGACTCTGGAGAGGAAGCGACCTGCCAGCATGGCAGTGATGGAAGGG GACTTGGTGAAGAAGGAGAGTCCTCCAAAACCCAAAGACTCCGTGTCTGCAGCTGTCCCTGCAGCAGGAA GGAACAGCAACCAGATGACCACGGTCCCAAACCAGGCTCAGACAGGTGGCAACTCCCATCAGCTCTCAGT AAGCACACCTCACAGTGCAGCCGGTCCCAGCCCGCACACTCTGCGTCGGGCTGTGAAGAAACCTGCCCCC GCACCCCCCAAACCAGGAAACCTACCTCCTGGCCACCCTGGAGGACAGAGCTCTCCTGGCACAGGCACAT CCCCAAAGCCAAGCGCCCGAAGCCCATCACCGCCTCAGCAGCAGCAGCAGCAGCAACAACAGCAGCAACA ACAGCAGCAGCAGCAGACCCCAGGCATGCGCCGCTGCTCCAGCAGCCTGCCTCCCATCCAGGCACCCAGC CACCCACCACCACAGCCCCCCACACAGCCTCGGTTGGGTGAACAGGGACCAGAGCCAGGCCCCACGCCAC CTCAAACCCCCACACCACCTAGCACCCCACCCCTGGCCAAGCAGAATCCATCGCAAAGTGAGACCACACA GCTGCACGGAACCCTCCCAAGACCACGGCCAGTGCCCAAGCCCCGCAACCGGCCTAGCGTGCCACCACCC CCACATCCACCTGGCACCCACACGGTGGATGGTGGCCTTACATCCTCAGTGCCCACAGCCTCCAGAATCG TCACTGATGCCCTTCCCGGTGCCCTCACAGGTGGGGAAGGTTTCCAGAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR216395 representing NM_001122642
Red=Cloning site Green=Tags(s) MKKQFNRMKQLANQTVGRAEKTEVLSEDLLQIERRLDTVRSMCHHSHKRLIACFQGQHGTDAERRHKKLP LTALAQNMQEASAQLEESLLGKMLETCGDAENQLALELSQHEVFVEKEIMDPLYGIAEVEIPNIQKQRKQ LARLVLDWDSVRARWNQAHKSSGTNFQGLPSKIDTLKEEMDEAGNKVEQCKDQLAADMYNFMAKEGEYGK FFVTLLEAQADYHRKALAVLEKALPEMRAHQDKWAEKPAFGTPLEEHLKRSGREIALPIEACVMLLLETG MKEEGLFRIGAGASKLKKLKAALDCSTSHLDEFYSDPHAVAGALKSYLRELPEPLMTFSLYEEWTQVASV QDQDKKLQYLWTTCQKLPPQNFVNFRYLIKFLAKLAQTSDVNKMTPSNIAIVLGPNLLWAKQEGTLAEIA AATSVHVVAVIEPIIQHADWFFPGEVEFNVSEAFVPLATPNSNHSSHTGNDSDSGTLERKRPASMAVMEG DLVKKESPPKPKDSVSAAVPAAGRNSNQMTTVPNQAQTGGNSHQLSVSTPHSAAGPSPHTLRRAVKKPAP APPKPGNLPPGHPGGQSSPGTGTSPKPSARSPSPPQQQQQQQQQQQQQQQQQTPGMRRCSSSLPPIQAPS HPPPQPPTQPRLGEQGPEPGPTPPQTPTPPSTPPLAKQNPSQSETTQLHGTLPRPRPVPKPRNRPSVPPP PHPPGTHTVDGGLTSSVPTASRIVTDALPGALTGGEGFQN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001122642 |
| ORF Size | 2220 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001122642.1, NP_001116114.1 |
| RefSeq Size | 3317 bp |
| RefSeq ORF | 2223 bp |
| Locus ID | 70497 |
| UniProt ID | Q3UIA2 |
| MW | 81.5 kDa |
| Gene Summary | Rho GTPase-activating protein involved in the maintenance of tight junction by regulating the activity of CDC42, thereby playing a central role in apical polarity of epithelial cells. Specifically acts as a GTPase activator for the CDC42 GTPase by converting it to an inactive GDP-bound state. The complex formed with AMOT acts by regulating the uptake of polarity proteins at tight junctions, possibly by deciding whether tight junction transmembrane proteins are recycled back to the plasma membrane or sent elsewhere. Participates in the Ca(2+)-dependent regulation of exocytosis, possibly by catalyzing GTPase activity of Rho family proteins and by inducing the reorganization of the cortical actin filaments. Acts as a GTPase activator in vitro for RAC1 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221238 | Arhgap17 (untagged) - Mouse Rho GTPase activating protein 17 (Arhgap17), transcript variant 4, (10ug) |
CNY 9,600.00 |
|
| MG216395 | Arhgap17 (tGFP-tagged) - Mouse Rho GTPase activating protein 17 (Arhgap17) transcript variant 4, (10ug) |
CNY 8,170.00 |
|
| MR216395L3 | Lenti ORF clone of Arhgap17 (Myc-DDK-tagged) - Mouse Rho GTPase activating protein 17 (Arhgap17), transcript variant 4 |
CNY 9,310.00 |
|
| MR216395L4 | Lenti ORF clone of Arhgap17 (mGFP-tagged) - Mouse Rho GTPase activating protein 17 (Arhgap17), transcript variant 4 |
CNY 9,310.00 |
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