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Pde1c (NM_001159953) Mouse Tagged ORF Clone
CAT＃: MR216137
- TrueORF®
Pde1c (Myc-DDK-tagged) - Mouse phosphodiesterase 1C (Pde1c), transcript variant 4
ORF Plasmid: tGFP
"NM_001159953" in other vectors (4)
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CNY 7,030.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR216137 representing NM_001159953
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTCTCCAACCAAGGAAATTGAAGAATTCGAGAGCAACTCTCTGAAACACCTGCAACCTGAGCAGA TCGAGAAAATCTGGCTTCGGCTCCGCGGGCTGAGGAAATATAAGAAAACGTCCCAGAGGTTACGGTCTTT GGTCAAGCAATTAGAACGAGGAGAGGCCTCTGTGGTAGACCTTAAGAAGAATCTGGAATATGCAGCCACA GTGCTTGAATCTGTATATATTGATGAAACAAGGCGACTGCTGGATACAGAGGATGAGCTTAGTGACATTC AATCAGATGCTGTGCCTTCAGAAGTTCGGGATTGGCTGGCCTCCACCTTCACACGGCAGATGGGGATGAT GCTTAGGAGAAGTGATGAGAAGCCCAGGTTCAAGAGCATCGTCCATGCGGTGCAAGCTGGGATATTTGTG GAAAGAATGTACAGACGGACATCAAACATGGTTGGGCTGAGCTATCCACCGGCTGTAATCGATGCATTGA AGGATGTGGATACGTGGTCCTTCGATGTCTTTTCCCTCAATGAGGCCAGTGGAGATCATGCACTGAAGTT CATTTTCTATGAATTACTCACACGTTATGACCTGATCAGCCGTTTTAAGATACCGATTTCTGCACTTGTC TCATTTGTGGAGGCCCTCGAAGTGGGTTACAGCAAGCACAAAAATCCTTACCATAACCTGATGCATGCAG CTGACGTCACACAGACTGTGCATTACCTCCTTTATAAGACAGGAGTAGCAAACTGGCTGACAGAGCTGGA GATCTTTGCAATAATCTTTTCGGCTGCCATCCATGACTATGAACATACTGGAACTACAAACAATTTCCAC ATTCAGACTCGGTCAGATCCAGCTATCCTGTACAATGACAGATCTGTCCTGGAGAACCACCACCTGAGTG CAGCCTACCGCCTTCTGCAGGAAGACGAAGAGATGAATATTCTGGTTAACCTCTCAAAGGATGACTGGAG GGAGTTTCGAACTTTGGTTATTGAGATGGTAATGGCCACAGATATGTCCTGTCATTTCCAGCAAATCAAA GCCATGAAGACAGCCCTGCAGCAACCAGAAGCAATTGAGAAGCCGAAAGCCTTATCCCTCATGTTACACA CAGCAGACATCAGTCATCCTGCGAAAGCATGGGACCTGCACCACCGCTGGACCATGTCTCTCCTGGAGGA GTTCTTTAGACAGGGTGACAGAGAAGCAGAGCTGGGGCTGCCATTTTCTCCTCTTTGTGACAGAAAGTCA ACCATGGTTGCTCAGTCACAAGTGGGTTTTATTGACTTCATTGTGGAGCCCACCTTCACTGTGCTCACGG ACATGACCGAGAAGATTGTGAGTCCATTAATTGACGAAAGCTCCCAGACTGGTGGGACAGGGCAGAGGAG ATCAAGTTTGAACAGCATCAACTCATCAGATGCAAAGCGATCCGGTGTCAAGAGTTCTGGGTCAGATGGA AGTGCTCCCATCAACAATTCTGTCATTCCTGTTGACTATAAGAGTTTTAAAGCCACTTGGACTGAGGTGG TGCAGATCAATCGGGAGCGGTGGCGAGCCAAGGTACCCAAAGAAGAAAAAGCCAAGAAGGAAGCTGAAGA GAAGGCTCGCCTGGCTGCTGAGGAAAAGCAAAAGGAAATGGAAGCCAAAAGCCAAGCTGAACAAGGCACA ACCAGCAAAGGCGAGAAAAAGACATCAGGAGAAGCCAAAAGTCAAGTCAATGGAACACGCAAGGGTGATA ACCCTCGTGGGAAGAACTCCAAAGGAGAAAAGGCAGGCGAAAAGCAGCAGAACGGTGACTTGAAAGACGG TAAAAATAAGGCAGACAAGAAGGATCACTCCAACACCGGAAATGAGTCAAAGAAAACAGATGGTACAAAG AAGCGTTCTCATGGCTCACCTGCTCCGAGCACTAGTTCCACAAGTCGCATTACCTTGCCAGTCATCAAGC CTCCTTTGCGTCACTTTAAGCGTCCTGCTTATGCATCTAGCTCCTATGCACCTTCCGTCCCGAAGAAAAC TGATGATCATCCTGTAAGATACAAGATGCTGGATCAGCGGATCAAGATGAAAAAGATCCAGAACATCTCT CATCACTGGAACAAAAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR216137 representing NM_001159953
Red=Cloning site Green=Tags(s) MESPTKEIEEFESNSLKHLQPEQIEKIWLRLRGLRKYKKTSQRLRSLVKQLERGEASVVDLKKNLEYAAT VLESVYIDETRRLLDTEDELSDIQSDAVPSEVRDWLASTFTRQMGMMLRRSDEKPRFKSIVHAVQAGIFV ERMYRRTSNMVGLSYPPAVIDALKDVDTWSFDVFSLNEASGDHALKFIFYELLTRYDLISRFKIPISALV SFVEALEVGYSKHKNPYHNLMHAADVTQTVHYLLYKTGVANWLTELEIFAIIFSAAIHDYEHTGTTNNFH IQTRSDPAILYNDRSVLENHHLSAAYRLLQEDEEMNILVNLSKDDWREFRTLVIEMVMATDMSCHFQQIK AMKTALQQPEAIEKPKALSLMLHTADISHPAKAWDLHHRWTMSLLEEFFRQGDREAELGLPFSPLCDRKS TMVAQSQVGFIDFIVEPTFTVLTDMTEKIVSPLIDESSQTGGTGQRRSSLNSINSSDAKRSGVKSSGSDG SAPINNSVIPVDYKSFKATWTEVVQINRERWRAKVPKEEKAKKEAEEKARLAAEEKQKEMEAKSQAEQGT TSKGEKKTSGEAKSQVNGTRKGDNPRGKNSKGEKAGEKQQNGDLKDGKNKADKKDHSNTGNESKKTDGTK KRSHGSPAPSTSSTSRITLPVIKPPLRHFKRPAYASSSYAPSVPKKTDDHPVRYKMLDQRIKMKKIQNIS HHWNKK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001159953 |
| ORF Size | 2118 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001159953.1, NP_001153425.1 |
| RefSeq Size | 3907 bp |
| RefSeq ORF | 2121 bp |
| Locus ID | 18575 |
| UniProt ID | Q64338 |
| MW | 80.7 kDa |
| Gene Summary | Calmodulin-dependent cyclic nucleotide phosphodiesterase with a dual-specificity for cAMP and cGMP, which are key regulators of many important physiological processes (PubMed:8810348). Exhibits high affinity for both cAMP and cGMP (By similarity). Modulates the amplitude and duration of the cAMP signal in sensory cilia in response to odorant stimulation, hence contributing to the generation of action potentials. Regulates smooth muscle cell proliferation. Regulates the stability of growth factor receptors, including PDGFRB (Probable).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220741 | Pde1c (untagged) - Mouse phosphodiesterase 1C (Pde1c), transcript variant 4, (10ug) |
CNY 7,768.00 |
|
| MG216137 | Pde1c (tGFP-tagged) - Mouse phosphodiesterase 1C (Pde1c) transcript variant 4, (10ug) |
CNY 7,700.00 |
|
| MR216137L3 | Lenti ORF clone of Pde1c (Myc-DDK-tagged) - Mouse phosphodiesterase 1C (Pde1c), transcript variant 4 |
CNY 8,930.00 |
|
| MR216137L4 | Lenti ORF clone of Pde1c (mGFP-tagged) - Mouse phosphodiesterase 1C (Pde1c), transcript variant 4 |
CNY 8,930.00 |
