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Lcmt2 (NM_177846) Mouse Tagged ORF Clone
CAT＃: MR215677
- TrueORF®
Lcmt2 (Myc-DDK-tagged) - Mouse leucine carboxyl methyltransferase 2 (Lcmt2)
ORF Plasmid: tGFP
"NM_177846" in other vectors (4)
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CNY 5,024.00
CNY 5,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | D330024M17; Tyw4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR215677 representing NM_177846
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCCCGCGAGGCCGCCAGCGCCGGGCGGGAACGGTCCAGAGCACCAACGACAGCAGCTCCCTCAGCA AACGATCGCTTGCCGCACACGGATACGTGCGCGACCCCTTTGCGGCGCTGCTGGTCCCGGGACCCGTGCG GCGCACGCCGCTTATCCACCGCGGCTATTACGTGCGCGCGCGCGCCGTGCGTCACTGCGTGCGCGCCTTC CTCGAACTCACTAGCGCGCTCCCGTCCCGGACCAGAGCCCAGATCCTGTCCTTGGGCTCAGGCTCCGACT CGCTTTATTTTCGTCTGAAAGCGGCGGGCCTCCTGGCCCGAGCTGCCGTCTGGGAGGTGGACTTCCCGGA CGTGTCTCGGCTCAAGGCGGAGAGGATCGAGGAGACCCCGGAGCTGCGTGCGCAGACCGGTCCTTTCAAG ATCGGGGACTCGGCGTCATCTCTGTGCTTCGAGAGCGCGGACTACCGCATCCTGGGCGCGGACCTGCGGG AGCTCCAGCGATTAGGAGAGGCCCTGGATGGCGCCGGCCTGGACGCCACCTCTCCCACGCTGCTCCTGGC CGAGGCGGTGCTGACCTACTTGGAGCCCTCCAGTGCCACAGCCCTCATTGCCTGGGCCGCCCAGCGTTTC CCCGACGCCCTTTTCGTGATCTATGAGCAGATGCAGCCGGGGGATGCCTTTGGGCAGATCATGCTGCAGC ACTTTCAGCGGTTGCATTCACCCTTGCATGGCCTGGAGCTGTTTCCCGTCGTGAAGGCCCAGCGCCAGCG CTTCCTTCAAGCTGGCTGGACTGCCTGCAGCGCCCTGGACCTGAACGAGTTCTATCGCCGCCTTCTCTCG GCAGAAGAGCGCCAGCGGGTTGAGACGCTTGAACCCTTTGATGAATATGAGGAGTGGCATCTGAAGTGTT CACATTATTTCATCCTGGCAGCATCTAGGGGAGACATTCTGTCAGAAACTCCAGTGTTTGAGCCCTCAGA GGCTTCTTTTCAGATAGATCCTGCCTCGCCTTCAGGGTTTCTTTCTGCCAGAGTAGTCACTAGTGACCAC CAGCACTCAAGCCTGAAGCGATATGGCCACGCCTCTGCCCTCTTGAGCCCTGGCGTTATTTTCAGTGCAG GAGGCTTTGGAGAACAAGAGGGACGACACTGCCGAGTGAGCAGGTTTCACGTGCTCTCCAGATCCTGTGA CTCGGAATGGGAAGGCTGCCAAATAAGTACCTTGGGGACTGAAGGCCAGTGGGATGGACGCCTTTATCAC ACCATGACAAGGCTTTCTGATACTCGGGTTCTGGTTCTTGGAGGAAGACTCTCCCCAGTAAGTCCAGCCT CTGGGGCTCTCCAACTTGATTTATACAAAAGTAAGGATAATTGCTCTGAGGGCCAAAATGTAACAGTAAC GAAGGCTGCCTTGGAAGAAGGTTCAGTGTTGTCATGTTGGCGGCATTCAACAACGGAAGTATACTATCAG AATCAAAGATACTTATTTGTGTATGGTGGCCGAAGTGTGGCTGAACCTGTACTAAGTGACTGTCGTTTTC TACATGTAGAGACAATGGCTTGGGTCAGAATCCCAGTACAAGGCGCATCACCCGAAGGTCGGCATTCCCA TAGCGCCTGCAGTTGGCAAGGGGGAGCACTTATCGCTGGAGGTCTTGGGGCTTCTGAGGAACTGTTGAGC TCTGTACTCTTTCTCAAACCAGTGTCCTCTGGATTCCTCTGGGAATCAATAGATATCCAGCCCTCCATTA CCCCAAGGTACTCTCACACGGCTCATGTGTTTAATGGAAAGCTGCTTCTGGTTGGAGGGGTCTGGATTCA TTCCTCCTCAGTTCCTGGAGTGACTGTTATCTGTTTGACTACAGGGTTGAGCTCTGAATATCAGATTGAC ACAGCGTCTGTGCCATGGCCATTAATGCTGCACAACCACAGCAGCGCCCTCCTTCCTGAAGAGCAGCAGC TCCTGCTTATTGGAGGTGGAGGGAACTGCTTTTCCTTTGGTACTTATTTCAACCCCCACACAGTGGCATT AGACCTTTCTTCCTTACGTTCAGGGCAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR215677 representing NM_177846
Red=Cloning site Green=Tags(s) MGPRGRQRRAGTVQSTNDSSSLSKRSLAAHGYVRDPFAALLVPGPVRRTPLIHRGYYVRARAVRHCVRAF LELTSALPSRTRAQILSLGSGSDSLYFRLKAAGLLARAAVWEVDFPDVSRLKAERIEETPELRAQTGPFK IGDSASSLCFESADYRILGADLRELQRLGEALDGAGLDATSPTLLLAEAVLTYLEPSSATALIAWAAQRF PDALFVIYEQMQPGDAFGQIMLQHFQRLHSPLHGLELFPVVKAQRQRFLQAGWTACSALDLNEFYRRLLS AEERQRVETLEPFDEYEEWHLKCSHYFILAASRGDILSETPVFEPSEASFQIDPASPSGFLSARVVTSDH QHSSLKRYGHASALLSPGVIFSAGGFGEQEGRHCRVSRFHVLSRSCDSEWEGCQISTLGTEGQWDGRLYH TMTRLSDTRVLVLGGRLSPVSPASGALQLDLYKSKDNCSEGQNVTVTKAALEEGSVLSCWRHSTTEVYYQ NQRYLFVYGGRSVAEPVLSDCRFLHVETMAWVRIPVQGASPEGRHSHSACSWQGGALIAGGLGASEELLS SVLFLKPVSSGFLWESIDIQPSITPRYSHTAHVFNGKLLLVGGVWIHSSSVPGVTVICLTTGLSSEYQID TASVPWPLMLHNHSSALLPEEQQLLLIGGGGNCFSFGTYFNPHTVALDLSSLRSGQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_177846 |
| ORF Size | 2058 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_177846.3, NP_808514.2 |
| RefSeq Size | 3407 bp |
| RefSeq ORF | 2061 bp |
| Locus ID | 329504 |
| UniProt ID | Q8BYR1 |
| MW | 75.3 kDa |
| Gene Summary | Probable S-adenosyl-L-methionine-dependent methyltransferase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA (By similarity). May methylate the carboxyl group of leucine residues to form alpha-leucine ester residues.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220477 | Lcmt2 (untagged) - Mouse leucine carboxyl methyltransferase 2 (Lcmt2), (10ug) |
CNY 8,740.00 |
|
| MG215677 | Lcmt2 (tGFP-tagged) - Mouse leucine carboxyl methyltransferase 2 (Lcmt2), (10ug) |
CNY 6,370.00 |
|
| MR215677L3 | Lenti ORF clone of Lcmt2 (Myc-DDK-tagged) - Mouse leucine carboxyl methyltransferase 2 (Lcmt2) |
CNY 7,700.00 |
|
| MR215677L4 | Lenti ORF clone of Lcmt2 (mGFP-tagged) - Mouse leucine carboxyl methyltransferase 2 (Lcmt2) |
CNY 7,700.00 |
