Ank3 (NM_170689) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	2900054D09Rik; AI314020; An; Ank; Ank-3; AnkG; Anky; Ankyrin-3; Ankyrin-G
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR215181 representing NM_170689 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGAAGAGCCAAAGGAGAAGCCCGCCAAGCCTGCTCATAGGAAGAGGAAAGGAAAAAAGTCTGATG CCAACGCAAGTTACTTAAGAGCAGCTCGGGCAGGGCACCTGGAAAAGGCCCTTGACTACATCAAAAATGG AGTGGACGTCAACATCTGTAACCAGAATGGATTGAATGCACTCCATCTTGCTTCCAAAGAAGGCCATGTG GAAGTGGTCTCTGAGCTGCTGCAGAGGGAAGCCAATGTTGATGCCGCCACAAAGAAAGGAAACACGGCCT TACACATCGCATCTTTGGCTGGGCAAGCGGAAGTGGTCAAGGTCTTGGTTACGAACGGAGCGAATGTCAA CGCACAATCTCAGAATGGCTTCACACCATTGTATATGGCAGCCCAGGAGAACCACCTGGAAGTCGTCAGG TTTCTTCTGGACAATGGCGCCAGCCAAAGCCTGGCCACAGAGGACGGCTTCACGCCATTGGCCGTGGCTC TGCAACAAGGTCATGACCAAGTCGTGTCCCTCCTGCTCGAGAACGACACGAAGGGAAAAGTGCGCCTCCC AGCCCTCCACATCGCAGCCCGGAAAGACGACACCAAGGCAGCAGCTCTGCTCCTGCAGAATGACACAAAC GCGGACGTGGAGTCAAAGAGTGGCTTCACCCCGCTCCACATAGCTGCCCACTATGGGAACATCAATGTGG CCACGTTGCTGTTAAACCGAGCGGCTGCTGTGGACTTCACCGCACGGAATGACATCACTCCCTTACACGT TGCCTCGAAGCGAGGAAATGCAAATATGGTGAAGCTATTGCTGGACCGGGGTGCGAAGATCGATGCCAAG ACCAGGGACGGTCTGACTCCGTTGCACTGTGGGGCGAGAAGTGGCCATGAGCAGGTGGTAGAGATGTTGC TTGACAGATCCGCCCCCATCCTTTCAAAAACCAAGAATGGATTGTCGCCACTGCACATGGCCACACAAGG AGACCATTTAAACTGCGTCCAACTCCTCCTCCAGCACAACGTGCCCGTGGACGACGTCACCAACGACTAC CTGACTGCCCTCCATGTGGCTGCCCACTGCGGCCATTACAAAGTTGCCAAGGTTCTTTTGGATAAGAAAG CTAGCCCCAATGCCAAAGCCCTGAATGGCTTCACCCCTCTCCATATCGCCTGCAAAAAGAACCGCATCCG AGTAATGGAACTCCTTTTGAAGCACGGTGCATCTATTCAAGCCGTAACCGAGTCGGGCCTTACCCCAATC CATGTTGCTGCCTTCATGGGACATGTAAATATCGTGTCACAGCTAATGCATCATGGAGCCTCCCCAAACA CCACCAATGTGAGAGGAGAGACGGCATTGCATATGGCGGCTCGGTCCGGACAAGCAGAAGTGGTGCGGTA TCTGGTCCAAGATGGGGCTCAGGTAGAAGCAAAAGCTAAGGATGACCAGACTCCACTCCACATCTCAGCC CGACTTGGGAAAGCTGACATAGTGCAACAACTGTTACAGCAAGGAGCATCCCCCAATGCAGCAACAACTT CTGGGTACACCCCCCTTCACCTTGCGGCCAGAGAGGGGCATGAGGATGTAGCTGCGTTCCTCCTGGATCA TGGAGCATCTTTATCCATAACAACAAAGAAGGGATTCACCCCTCTGCACGTGGCAGCCAAATACGGAAAG CTTGAAGTCGCAAGTCTCCTGCTGCAGAAGAGTGCGTCTCCCGATGCCGCAGGGAAGAGCGGGCTAACTC CACTGCATGTAGCAGCGCATTACGATAATCAGAAAGTGGCCCTTCTGCTCTTGGACCAGGGAGCCTCACC CCACGCAGCCGCAAAGAATGGCTATACACCACTGCACATCGCGGCCAAGAAGAACCAGATGGACATAGCC ACGTCCCTGCTGGAGTACGGTGCTGATGCAAACGCGGTTACCCGGCAAGGGATTGCGTCCGTCCATCTTG CGGCACAGGAAGGGCACGTGGACATGGTGTCGCTGCTCCTGAGTAGAAACGCGAATGTCAACCTGAGCAA TAAGAGCGGTCTCACCCCACTCCACCTGGCTGCTCAAGAAGACCGAGTGAATGTGGCCGAGGTCCTTGTC AACCAGGGGGCCCATGTGGATGCTCAGACAAAGATGGGCTACACCCCGCTCCATGTGGGCTGTCACTATG GAAATATCAAAATAGTCAATTTTCTGCTGCAGCATTCTGCAAAAGTTAATGCCAAGACGAAGAATGGATA CACAGCACTGCACCAGGCTGCTCAGCAGGGCCACACGCATATCATCAATGTCTTGCTTCAGAACAACGCC TCCCCCAATGAACTCACTGTGAATGGGAACACAGCTCTGGCCATCGCCCGGCGCCTTGGTTACATCTCGG TGGTTGACACACTGAAGGTCGTGACGGAGGAAATTATGACCACCACTACCATCACGGAGAAGCACAAAAT GAATGTCCCAGAAACGATGAATGAAGTCCTCGATATGTCAGACGATGAAGTAAGGAAAGCCAGCGCCCCC GAAAAGCTCAGTGATGGGGAATATATCTCAGACGGTGAAGAAGGTGAAGATGCCATCACAGGGGACACTG ACAAGTATCTCGGGCCACAGGACCTTAAGGAGCTAGGTGATGACTCCCTGCCAGCAGAAGGTTACGTAGG CTTCAGTCTTGGAGCCCGTTCTGCCAGCCTCCGCTCCTTCAGTTCGGATAGGTCCTACACCTTGAACAGA AGCTCCTACGCAAGGGACAGCATGATGATAGAGGAACTTCTGGTACCATCCAAAGAGCAGCACCTGACGT TCACGAGGGAGTTTGATTCTGACTCCCTCAGACACTACAGTTGGGCAGCGGACACGTTAGATAATGTGAA CCTGGTCTCAAGCCCGGTGCATTCTGGGTTTCTGGTTAGCTTTATGGTGGACGCGAGAGGGGGCTCCATG CGAGGAAGCCGCCACCACGGGATGCGGATCATCATCCCTCCGCGAAAGTGTACGGCCCCCACCCGCATCA CGTGCCGCCTGGTAAAGAGACATAAACTGGCCAACCCACCCCCCATGGTGGAAGGAGAGGGATTAGCCAG TAGGCTGGTAGAAATGGGTCCTGCGGGGGCACAATTTTTAGGCCCCGTCATTGTGGAAATCCCTCATTTT GGGTCCATGAGGGGGAAGGAGAGAGAACTTATCGTCCTTCGGAGCGAGAACGGAGAGACCTGGAAGGAAC ATCAGTTTGACAGTAAAAACGAAGACCTCGCGGAGCTTCTCAATGGCATGGATGAAGAACTCGACAGCCC GGAAGAGTTGGGTACAAAGCGCATCTGCAGAATTATCACAAAGGATTTCCCCCAGTATTTTGCCGTGGTT TCCCGGATTAAGCAGGAAAGCAACCAGATCGGTCCTGAGGGTGGGATTCTGAGCAGCACCACCGTGCCCC TCGTCCAGGCCTCCTTCCCAGAGGGCGCCTTAACCAAGAGGATCCGTGTGGGTCTCCAGGCTCAGCCCGT GCCAGAGGAAACGGTAAAAAAAATCCTTGGGAACAAAGCAACATTTAGCCCAATTGTCACGGTAGAGCCG AGGAGAAGGAAGTTCCATAAGCCGATCACCATGACCATTCCGGTGCCCCCGCCCTCGGGAGAAGGCGTGT CCAATGGGTACAAGGGGGATGCCACGCCCAACCTGCGGCTCCTCTGCAGCATCACAGGAGGCACCTCACC AGCTCAATGGGAAGACATCACAGGAACAACCCCTCTGACGTTCATAAAGGATTGTGTGTCTTTCACAACC AACGTTTCAGCCAGATTCTGGCTGGCGGACTGCCATCAGGTGTTAGAGACCGTAGGGCTAGCCTCCCAGC TGTACAGAGAGCTGATATGCGTTCCCTACATGGCCAAGTTCGTTGTGTTTGCCAAAACAAACGACCCGGT GGAGTCCTCGCTGAGGTGCTTCTGTATGACAGACGACAGGGTGGACAAAACCCTGGAGCAGCAGGAGAAC TTCGAGGAGGTTGCCAGAAGCAAAGACATTGAGGTTCTGGAAGGAAAGCCCATCTACGTTGATTGCTATG GAAACCTGGCCCCTCTGACCAAAGGAGGACAGCAGCTTGTTTTTAACTTTTATTCTTTCAAAGAAAACAG ACTGCCATTTTCCATCAAGATCAGAGACACCAGTCAAGAGCCCTGTGGCCGCCTGTCTTTCCTGAAGGAG CCAAAGACAACAAAGGGATTACCCCAAACAGCTGTTTGCAACTTAAATATTACTCTGCCGGCACATAAAA AGGCTGAGAAGGCAGACAGACGCCAGAGCTTTGCCTCCCTAGCTTTACGTAAGCGCTACAGCTACTTGAC TGAACCCAGCATGAGTCCGCAGAGTCCTTGTGAGCGGACGGATATCAGGATGGCGATAGTAGCCGATCAC CTGGGACTTAGTTGGACAGAGCTGGCAAGGGAACTGAATTTTTCAGTGGATGAAATCAACCAAATACGTG TGGAAAATCCCAATTCTTTAATTTCTCAGAGCTTCATGTTATTAAAAAAGTGGGTGACCAGAGACGGAAA GAATGCCACAACTGATGCCTTAACTTCGGTCTTAACGAAGATTAACCGGATAGACATTGTAACTCTGCTG GAAGGACCAATATTTGATTATGGGAATATTTCAGGCACCAGAAGCTTTGCAGATGAAAACAATGTTTTCC ATGACCCAGTTGATGGTCACCCTTCCTTTCAAGTGGAGCTGGAGACCCCCATGGGGTTGTACTGCACACC ACCCAACCCTTTCCAGCAAGATGACCATTTTAGTGATATCTCTAGCATAGAGTCTCCCTTTAGGACCCCC AGTAGACTGAGTGACGGGCTGGTGCCTTCCCAGGGAAACATAGAGCATCCAACAGGTGGACCTCCAGTGG TAACCGCAGAGGACACTTCTTTAGAAGACAGCAAAATGGACGATTCTGTAACTGTAACAGACCCGGCCGA CCCACTGGACGTAGATGAGAGCCAGTTGAAGGACCTGTGTCAGAGCGAGTGTGCTCAGTGCTGGGCGAGT GTGCCCGGGATCCCAAACGACGGTCGGCAGGCAGAGCCACTGAGACCGCAGACTAGAAAAGTAGGCATGA GCTCTGAACAGCAGGAAAAAGGAAAATCTGGTCCTGATGAGGAAGTGACAGAAGACAAGGTCAAATCTCT GTTTGAGGACATTCAACTTGAAGAAGTAGAGGCTGAGGAGATGACAGAAGACCAGGGGCAGGCTATGCTT AACCGTGTTCAGCGAGCAGAACTGGCAATGTCTTCACTTGCAGGTTGGCAGAACGAGACGCCAAGTGGAA GCCTAGAGTCCCCAGCGCAAGCTCGAAGACTAACTGGTGGGTTACTGGACCGTCTGGATGACAGCTCTGA CCAGGCTCGGGATTCTATTACCTCATACCTCACGGGAGAACCTGGGAAGATCGAAGCAAATGGAAACCAC ACAGCGGAAGTCATTCCAGAAGCAAAGGCAAAACCCTACTTCCCGGAATCCCAAAACGATATAGGGAAAC AGAGCATCAAGGAGAACCTGAAACCAAAAACACACGGATGTGGTCGCACTGAGGAACCAGTGTCGCCCCT CACAGCCTACCAGAAATCTCTGGAAGAAACCAGCAAGCTTGTCATAGAAGACGCACCTAAACCCTGTGTG CCTGTCGGCATGAAAAAGATGACCAGGACTACGGCTGACGGCAAAGCCAGGCTCAACCTCCAGGAAGAAG AGGGGTCCACCAGGTCAGAGCCTAAGCAGGGAGAAGGCTATAAGGTGAAGACGAAGAAGGAAATCCGGAA CGTGGAGAAGAAAACCCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR215181 representing NM_170689 Red=Cloning site Green=Tags(s) MSEEPKEKPAKPAHRKRKGKKSDANASYLRAARAGHLEKALDYIKNGVDVNICNQNGLNALHLASKEGHV EVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVR FLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRLPALHIAARKDDTKAAALLLQNDTN ADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAK TRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDY LTALHVAAHCGHYKVAKVLLDKKASPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPI HVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISA RLGKADIVQQLLQQGASPNAATTSGYTPLHLAAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGK LEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIA TSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLV NQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTALHQAAQQGHTHIINVLLQNNA SPNELTVNGNTALAIARRLGYISVVDTLKVVTEEIMTTTTITEKHKMNVPETMNEVLDMSDDEVRKASAP EKLSDGEYISDGEEGEDAITGDTDKYLGPQDLKELGDDSLPAEGYVGFSLGARSASLRSFSSDRSYTLNR SSYARDSMMIEELLVPSKEQHLTFTREFDSDSLRHYSWAADTLDNVNLVSSPVHSGFLVSFMVDARGGSM RGSRHHGMRIIIPPRKCTAPTRITCRLVKRHKLANPPPMVEGEGLASRLVEMGPAGAQFLGPVIVEIPHF GSMRGKERELIVLRSENGETWKEHQFDSKNEDLAELLNGMDEELDSPEELGTKRICRIITKDFPQYFAVV SRIKQESNQIGPEGGILSSTTVPLVQASFPEGALTKRIRVGLQAQPVPEETVKKILGNKATFSPIVTVEP RRRKFHKPITMTIPVPPPSGEGVSNGYKGDATPNLRLLCSITGGTSPAQWEDITGTTPLTFIKDCVSFTT NVSARFWLADCHQVLETVGLASQLYRELICVPYMAKFVVFAKTNDPVESSLRCFCMTDDRVDKTLEQQEN FEEVARSKDIEVLEGKPIYVDCYGNLAPLTKGGQQLVFNFYSFKENRLPFSIKIRDTSQEPCGRLSFLKE PKTTKGLPQTAVCNLNITLPAHKKAEKADRRQSFASLALRKRYSYLTEPSMSPQSPCERTDIRMAIVADH LGLSWTELARELNFSVDEINQIRVENPNSLISQSFMLLKKWVTRDGKNATTDALTSVLTKINRIDIVTLL EGPIFDYGNISGTRSFADENNVFHDPVDGHPSFQVELETPMGLYCTPPNPFQQDDHFSDISSIESPFRTP SRLSDGLVPSQGNIEHPTGGPPVVTAEDTSLEDSKMDDSVTVTDPADPLDVDESQLKDLCQSECAQCWAS VPGIPNDGRQAEPLRPQTRKVGMSSEQQEKGKSGPDEEVTEDKVKSLFEDIQLEEVEAEEMTEDQGQAML NRVQRAELAMSSLAGWQNETPSGSLESPAQARRLTGGLLDRLDDSSDQARDSITSYLTGEPGKIEANGNH TAEVIPEAKAKPYFPESQNDIGKQSIKENLKPKTHGCGRTEEPVSPLTAYQKSLEETSKLVIEDAPKPCV PVGMKKMTRTTADGKARLNLQEEEGSTRSEPKQGEGYKVKTKKEIRNVEKKTH TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_170689
ORF Size	5829 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_170689.2, NP_733790.2
RefSeq Size	9971 bp
RefSeq ORF	5832 bp
Locus ID	11735
UniProt ID	G5E8K5
MW	212.4 kDa
Gene Summary	This gene encodes a member of the ankyrin protein family. Ankyrins link integral membrane proteins to the spectrin-based cytoskeleton. Ankyrin family members share a protein structure which includes three independently folded domains: the N-terminal ankyrin repeat domain, the central spectrin-binding domain, and the C-terminal rod domain. This ankyrin functions as the major ankyrin in the kidney and may play a role in the polarized distribution of many integral membrane proteins to specific subcellular sites. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

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