Zfp407 (NM_001033341) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	6430585N13Rik; Gm333; Gm334; Gm948; ZNF407
Vector	pCMV6-AC-Myc-DDK
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR214555 representing NM_001033341. Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGATTCTGAGAATAAACATGACAATGATGAGGATGAAAGTGTTAAGGGAGCAAAAGATCCGAGGAGT GTTTCATCCCGCGATGACAGTTCTGAGCCTGTATCTGATGACATGGCAAATGTCTCTGAGAATTTTACG AGCAAAAGAGTTTTTTCCGAGTCGTCCAACTCGGACGGTGTTGCTGCGGAGGAATACAGAAGTGAGCGT GCTTCCAAGCGACTGAGAACAGAGGCACTGGAGCCTGAGACAAGAGAGCAGGGTGCCTGTGGGTTGGGC ACTGTGGAAGCTAATGTCCATTTGGCTGAAATGGGAAAGGAGGCCTTTCCAGCAAACTGCTCAGAAGGA GGCAGCGCTCTTCCCAGTGTTTTCTCCCCATCTTGTGATTTTAGCACATCTAATATTCTTCCTCTGAAA GTAGATGAAGTGAAAAAGCCTGCTCGGGAAATGGTTTCCCTTGTTCCTGAAAGACACAATCCTTTTCCT CCAGAAGAAAGGAGCATTAGTTGTAGCTTTGGAAGTGTAGAGACAGGACTCAGGTGCGGCGTGTGTGGC TGTTCATTTTCCTCTTGCTCTGCTTTGGAAAAGCATGTGGAGTGTCATGTGGAAGAGGGGAAGGAACGG ACCTGCTGCCACTGTAGCCACAGAGCAGAGAGCAGCTCATCTCCTCATGAGCATGTCAGACATACTCAT GGGCCGCAGAAAGTCTTTTCCTGTGATCTCTGTGGCTTCCAATGTGCAGAAGAAAACCTGTTGAATGCA CATTATCTTGGCAAAACACACCTTCGTCGTCAGAACCTTGCTGCTCGTGGAGGATTCGTACAGATCTTA ACAAAACCATCTTTTCCTAAAAAAGCCTGTGTCATGGGAACAAAAAATGTTAGAACAAAACCAAGAGCT TCTAAACCAATAGCAAAGACTGGGGATTCAAGAGTAGTGCCGAGTACTGGGAATGACTTTAAAGACTGC AGAGGAGCCCCTTCTGAAGACAGTGCTGGTGGTAGTGAGCTCCTTGTTGAAATGGTGCCATCTAGGAAA ATCTCATCAGGCAAAGCACATGTAGTTGAGGAAAATGTTAGTTTTGGTGTAGCTCAAAATCCTGAAAAT CAGAACAAACAGCTAGGGGGCTTAGTAAGCTCAGAGGGTCTTTTAAATAAACCAGAATCCACTAAAAAT GCCCTACAGATGGCGCATGTCAGTATCAGTACCACCTCAAGGCCAAGATCTGAGCGAAACCTCCTAGTG TTGGGTAATAGCTTTCGCAGACGAAGTGGCACTTTTACTTTAAAGGGCCAGGCAAAGAAAAGGTTTAAT CTTCTAGGAATTAATAAAAGGGGTACAAATGAAACTCAGAGGATGTATATGAAACACTTTAGAACACAG ATGAAAACAAACGCACAGCCAGTGCTGGAACAAGTAGAGATGAGCAAAGATGTTCAAAGTCTGTGTGTG ACTACCTCAGACAACCCAGAAGTGATGCAGGACAAGACAGCCTTCTGTCTTTCAGGCTCCACACGGCTG GGCTCCCTGCCAGTAAAGCCAGCTGACCACCAGCTCTCTGTGCAGTGCACTTGTACAGAGTGTGGCCAG ATAGCTGCAAATAAGACAGACTTCGAAGTTCACGTTAAACAGTGCCATGGAAGAGAGATGCAGTTTCAT TGTCAGACTTGTGACTTTTCTAGTCCGTCAAGGAGAGACTTGGAGGAGCACGTGCACAGTAATCAGCAT CAGCACATGACACCTGTCCTCAGCTGTCAGTGTTGTTCCTTTATTTCCTTGAATGAAACAGGTCTTAGA GACCATATGAAGGAGAAGCATGGCATGGGCTTTTTCTGCACCTCTTGTAATCTGTTCTTGTCTGAGAAA GATGTGGAAGAACACAGAGCAACCGAGTCACATAATAGTTTAGTGGTTCAACCAAAGACGGCTTCATCA TTGAGTGGTGATTCTGTTCTACCTTTCAGTACTGTAGAATCAGAAAACCCGGCCGATTCTAGAGAAGAC TCAGGGAAGGCCGCGCAGGAAGGACCTGCTGAGTCCAGGGCAAGCCATGGAACTGAAGCACGGCATTCA AGTAAGCCGCAGTTTCAGTGTAAGAAGTGTTTTTATAAAACGAGGTCCTCCACTGTTCTCACGAGACAC ATAAAGCTTCGACATGGACAGGACTATCATTTTCTCTGTAAAGCATGCAATCTTTATTCTTTGAGCAAA GAAGGAATGGAGAAGCACATTAAAAGAAGCAAACATCTTGAAAATGCTAAGAAAAACAATATTGGTTTA AGTTTTGAAGAGTGCATTGAAAGAGTATGTATAGGTGCAAATGATAAAAAAGAAGAGTCCAGTGTTTCT GGAAGTGGAAGGCCCGAAGGCCATGTTGGTGTGCAATCACAGGAGCATTCCCAACGTGAACAGAGTATG CTAACACCCAAGGAGCTTCCACAGTCTGGTGTCATCACCAAAGAGGATGAATTAGGCTTAGCCACTACT CCAAAGAGAGGGAGGCCCAAAGGCAGCATTTCTCGGACTTGTTCACACTGTGGACTTTTGGCCTCTAGT ATTACAAATTTGACTGTGCACATCAGGCGTAAACACAGTCACCAGTATAGTTACTTGTGCAAAGTATGT AAGTATTATACTGTAACTAAAGGAGATATGGAACGTCATTGTGCCACCAAGAAACATAAAGGACGGGTA GAAATAGAAGCAAATGGGAAACAAAGTTCAGACATAGTTGTTGGCCCAGAAGGTGGTAATCTTGAAGCA TGTAAGGATAGCACCTCTCTAGCAGTGACCGTTTCTGACGCACAGGCTAGCAAGCCGGCTAAGTCAGAC ACACGCACTTTAGAAACGCCGGGAGTGGGCATTGGAAATGCAGGTGATGCTGAAGCTGGGAGTGTATTT CCTTCTGGAGATGGAGAGCTTAGCAGTCAACTCTCTGATAAAAAGGGACAGCTATCTCTAGAGACAGAG GACCTGCTACAGCTTGATGATGCATGCTCTCAGAGAGAGGTTGCAGGTTCAAGTGACAATAAGTGTCTT CAGTGTGAGTTCAGTGCTCACTCTGCCGCTTCACTCGAGCTGCATGTGAAAAGGAAGCACACCAAAGAG TTCCAGTTTTACTGTATGGCCTGTGATTACTATGCGGTGACTCGCAGAGAGATGACGAGGCATGCTGCA ACAGAGAAGCATAGAATGAAAAGGCAGTCTTACCTCAGCGCTTCTAGTGTGGAAGCAGGTTCTTCAGAA ATATCCAAGAACATCACTATACCCGAGGAGGAGCATTCACACAATTCTGAGGAATTTCAAATACATCCA CACCAGTCATCTGGTACTCTCCAATGTAGAAATCCTGCAGATTGTTCTATTTTAGATGATAATACCAAT TTGGGTATGTCTAAAGTACTCTGTGCTCCTGATTCAGTGACAGTTGTGACTGAGCAAGAATCTAATTTT AGTGAAGGTCACTCCTTTTGTGAGACCTTACAGCAGCCCCTTGTTAAGGACAAAAGTATGAAACCCAGG GAGATAGTGTCCCCTAACACTCCCTCTAACCTCAGCCTCCCAGGCTGTCTTCAGAGTGAAAACCTAGCG AGCTCTGCTGTGGACTGTGAGACAGCAAAGAAAAACCGTGATGTGTTGGATGCTGTTGGGGATAGAAGT ACACCCTGTGAAGATGAGGGAGGCAGTGTGGATGATAGTGAAGAAAAAATTCTTGACAAGTCCCCCTGC CCTGGAGACCCAGATGGGGGCCATTCTGCCGAATCTACTTCCTCTGTGGTAATGAAAATACCAAGAGAA CAACTGGACCTGGATGGCGGTGGCCAGAACAAGGTTGGATGTGAGCAGACTTCTGAAGATTTAAAAGAT GTGCAAGCAAATCCTATTCTTGAGAATAAGGAAATTCTGATAAATTCACAAGAAGAAGCTGAAGTCATT TTGGAAGAGGATGCCCCAACCTCGAATGGCACAGCTGACAGTAATGATGTTTATGAGACTATAATAAGT ATTGATGACAAAGGGCAAACAATGTACAGTTTTGGCAGGTTTGATTCGTCCATAATTAGAATAAAAACT GAAGATGGTGAATTGGTAGAACAGCCAGAAGAGGGTCTGACGGCGACAGGAGGGAGAGTTAGTGAGTTG CCCCTGAAAGACTGTGCTCAGGGGTTGAAGAAGAAGAAAGTTGAAGGTGGTTCTTTTGGTGAGTCCACA CGAATCCGGTGTGATGATTGTGGTTTCTTAGCAGATGGACTGAGTGGCCTGAATGTTCATATAGCCATG AAGCATCCTACAAAAGAGAAGCACTTTCATTGTTTACTATGTGGAAAATCCTTCTATACTGAGAGCAAC CTTCACCAGCATTTGGCTAGTGCTGGTCACATGAGAAATGAACAGGCCAGTGTAGAAGAGCTTCCAGAA GGAGGGGCCACCTTCAAATGTGTCAAGTGTACAGAGCCCTTTGATTCGGAACAGAATTTATTTCTGCAT ATTAAAGGACAGCATGAGGAGTTGCTGCGGGAGGTAAACAAGTACATAGTGGAAGACACCGAGCAGATC AATCGGGAGAGAGAGGAGAACCAGGGCAACGTCTGCAAGTATTGTGGGAAGATGTGTCGGAGTAGCAAC TCCATGGCCTTTCTGGCTCACATTCGGACTCACACAGGATCAAAACCATTCAAGTGCAAGATATGCCAT TTTGCAACAGCTCAGCTTGGAGATGCCAGAAACCATGTGAAAAGGCACCTTGGGATGAGGGAGTACAAG TGTCACGTCTGTGGGGTTGCTTTTGTGATGAAGAAACACTTAAATACTCACCTGCTAGGCAAGCACGGA GTTGGCACCCCAAAGGAAAGGAAATTTACATGCCACTTATGTGATAGAAGTTTCACGGAGAAATGGGCC TTGAACAACCACATGAAACTGCACACGGGAGAAAAGCCTTTTAAGTGCACCTGGCCCACGTGCCACTAC TCATTCCTCACAGCATCAGCGATGAAAGACCACTACAGGACGCACACAGGCGAGAAGTCGTTTCTCTGT GACCTCTGTGGCTTCGCCGGTGGGACCCGGCATGCCCTCACCAAGCACCGTCGGCAGCATACAGGGGAA AAGCCTTTCAAATGTGATGAGTGTAACTTCGCCTCCACAACCCAGTCACACTTGACTCGTCATAAGCGT GTGCACACTGGAGAGAAGCCATACAGGTGCCCTTGGTGTGACTACAGGTCAAATTGTGCTGAGAATATC CGCAAACACATTTTACACACCGGCAAACATGAAGGTGTCAAGATGTACAACTGCCCAAAATGCGACTAT GGGACGAATGTCCCCGTGGAGTTCCGAAACCACCTGAAAGAGCAGCACCCCGACATTGAGAACCCTGAC CTCGCGTACTTGCATGCTGGCATCGTGTCCAAGTCCTATGAGTGCCGCCTGAAAGGACAAGGAGCCACG TTTGTGGAGACAGACAGCCCCTTCACTGCAGCCACCCTGGCGGAGGAGTCGCCTGTCAAGGAGAGGTCC TTGAGGAGCAGCAAGCGGCAGGCTGCGTCCCCTGAGCAGGTCCAGCAGGTCATCATCATCCAGGGCTAC GATGGCGAGTTCGCCCTGGACGCCTCCGTGGAAGAGACTGCTGCGGCCACACTGCAGACGCTGGCGATG GCTGGCCAAGTGGCCAGGGTGGTGCACATCACAGAACATGGGCAGGTCATTGCCACAAGTCAGAACGGG TCGCATGTGGGCAGTGTGGTACCTGGACCCATCCTCCCTGAGCAGTTGGCAGACGGAACCACCCAGGTG GTTGTCATGGGGGGTTCCATGGAAAGTCACAGTGTGGACGAGGCCCTCAGCCCAGGTGCTGCTGTCATA CAGCAGGTCACCAAGCAGGAGGTCTTAAGTCTCTCTGAGGCTGGAGTCCCTCCATCTGACAACTCCTCA GCCTTAGATGCACTGCTGTGTGCTGTCACTGAGTTGGGAGAGGTGGAGGGCAGAGTGGGGCATGAGGAA AAAGGTCGGCCCAGCCACAAGGACGTGCTGATCCAGCTGCCCAGCCAGGAAGCGGCCCAGGCTCACGCC AAGGCGGAGGCCACGGAGGCTCAGCTGTTCCAAGATGTTGAGGAGAGTCCAGCATCCATGGAAGTACTG ACCCAGGTTGTCCGCCCCTCAACCATCATCACGTCTCAGGAACGAGCCCAGGTGGCCTTCAAGAAGATG GTTCAGGGAGTCCTGCAGTTTGCTGTCTGTGACACGGCTGCAGCTAGCCAGCTGATAAAGGATGGCGTT ACTCAAGTCATTGTGAATGAAGAGGGCGCTGTCCACATGGTGGCCGGAGAAGGCTCCCAGTTCATCATG CAGGAGGCTGAGACCCATGGCCTGCGGGTGCCAGCCGAGCACATGGACCTGGTAGAGTCAGAAGGAGAG ATCTCTCAGATCATTGTAACAGAAGAGCTAGTCCAAGCCATGGTCCGTGAGTCCAACAGCAGCTTCCCC GAGGGCGCCACTCACTACATTGTGACCGAGTTGCCTCCGGGTGTACAGGAGGACACAGGAGTTTACTCC CACACTGTGATAGAGACAGCCAGCTCTCCAGAGATTCTGCAGGCTGGGGCTGCACTCAGTGCTGAGGCT GTGGGTTCCAGCAGCACGGAACAGTTGACGAGCATGGTCATCTATACCCAGGATGGCTCCCCAGCAGCC ACAGTGATCCAGAGCCAAAGAGAAAACAGTGAGCTCCAAGAAGCG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC Protein Sequence (showhide) >Peptide sequence encoded by MR214555 Blue=ORF Red=Cloning site Green=Tag(s) MDSENKHDNDEDESVKGAKDPRSVSSRDDSSEPVSDDMANVSENFTSKRVFSESSNSDGVAAEEYRSER ASKRLRTEALEPETREQGACGLGTVEANVHLAEMGKEAFPANCSEGGSALPSVFSPSCDFSTSNILPLK VDEVKKPAREMVSLVPERHNPFPPEERSISCSFGSVETGLRCGVCGCSFSSCSALEKHVECHVEEGKER TCCHCSHRAESSSSPHEHVRHTHGPQKVFSCDLCGFQCAEENLLNAHYLGKTHLRRQNLAARGGFVQIL TKPSFPKKACVMGTKNVRTKPRASKPIAKTGDSRVVPSTGNDFKDCRGAPSEDSAGGSELLVEMVPSRK ISSGKAHVVEENVSFGVAQNPENQNKQLGGLVSSEGLLNKPESTKNALQMAHVSISTTSRPRSERNLLV LGNSFRRRSGTFTLKGQAKKRFNLLGINKRGTNETQRMYMKHFRTQMKTNAQPVLEQVEMSKDVQSLCV TTSDNPEVMQDKTAFCLSGSTRLGSLPVKPADHQLSVQCTCTECGQIAANKTDFEVHVKQCHGREMQFH CQTCDFSSPSRRDLEEHVHSNQHQHMTPVLSCQCCSFISLNETGLRDHMKEKHGMGFFCTSCNLFLSEK DVEEHRATESHNSLVVQPKTASSLSGDSVLPFSTVESENPADSREDSGKAAQEGPAESRASHGTEARHS SKPQFQCKKCFYKTRSSTVLTRHIKLRHGQDYHFLCKACNLYSLSKEGMEKHIKRSKHLENAKKNNIGL SFEECIERVCIGANDKKEESSVSGSGRPEGHVGVQSQEHSQREQSMLTPKELPQSGVITKEDELGLATT PKRGRPKGSISRTCSHCGLLASSITNLTVHIRRKHSHQYSYLCKVCKYYTVTKGDMERHCATKKHKGRV EIEANGKQSSDIVVGPEGGNLEACKDSTSLAVTVSDAQASKPAKSDTRTLETPGVGIGNAGDAEAGSVF PSGDGELSSQLSDKKGQLSLETEDLLQLDDACSQREVAGSSDNKCLQCEFSAHSAASLELHVKRKHTKE FQFYCMACDYYAVTRREMTRHAATEKHRMKRQSYLSASSVEAGSSEISKNITIPEEEHSHNSEEFQIHP HQSSGTLQCRNPADCSILDDNTNLGMSKVLCAPDSVTVVTEQESNFSEGHSFCETLQQPLVKDKSMKPR EIVSPNTPSNLSLPGCLQSENLASSAVDCETAKKNRDVLDAVGDRSTPCEDEGGSVDDSEEKILDKSPC PGDPDGGHSAESTSSVVMKIPREQLDLDGGGQNKVGCEQTSEDLKDVQANPILENKEILINSQEEAEVI LEEDAPTSNGTADSNDVYETIISIDDKGQTMYSFGRFDSSIIRIKTEDGELVEQPEEGLTATGGRVSEL PLKDCAQGLKKKKVEGGSFGESTRIRCDDCGFLADGLSGLNVHIAMKHPTKEKHFHCLLCGKSFYTESN LHQHLASAGHMRNEQASVEELPEGGATFKCVKCTEPFDSEQNLFLHIKGQHEELLREVNKYIVEDTEQI NREREENQGNVCKYCGKMCRSSNSMAFLAHIRTHTGSKPFKCKICHFATAQLGDARNHVKRHLGMREYK CHVCGVAFVMKKHLNTHLLGKHGVGTPKERKFTCHLCDRSFTEKWALNNHMKLHTGEKPFKCTWPTCHY SFLTASAMKDHYRTHTGEKSFLCDLCGFAGGTRHALTKHRRQHTGEKPFKCDECNFASTTQSHLTRHKR VHTGEKPYRCPWCDYRSNCAENIRKHILHTGKHEGVKMYNCPKCDYGTNVPVEFRNHLKEQHPDIENPD LAYLHAGIVSKSYECRLKGQGATFVETDSPFTAATLAEESPVKERSLRSSKRQAASPEQVQQVIIIQGY DGEFALDASVEETAAATLQTLAMAGQVARVVHITEHGQVIATSQNGSHVGSVVPGPILPEQLADGTTQV VVMGGSMESHSVDEALSPGAAVIQQVTKQEVLSLSEAGVPPSDNSSALDALLCAVTELGEVEGRVGHEE KGRPSHKDVLIQLPSQEAAQAHAKAEATEAQLFQDVEESPASMEVLTQVVRPSTIITSQERAQVAFKKM VQGVLQFAVCDTAAASQLIKDGVTQVIVNEEGAVHMVAGEGSQFIMQEAETHGLRVPAEHMDLVESEGE ISQIIVTEELVQAMVRESNSSFPEGATHYIVTELPPGVQEDTGVYSHTVIETASSPEILQAGAALSAEA VGSSSTEQLTSMVIYTQDGSPAATVIQSQRENSELQEA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001033341
ORF Size	6738 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001033341.2, NP_001028513.2
RefSeq Size	7909 bp
RefSeq ORF	6741 bp
Locus ID	240476
MW	246 kDa

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