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Top2b (NM_009409) Mouse Tagged ORF Clone
CAT＃: MR212050
- TrueORF®
Top2b (Myc-DDK-tagged) - Mouse topoisomerase (DNA) II beta (Top2b)
ORF Plasmid: tGFP
"NM_009409" in other vectors (4)
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CNY 16,060.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | D230016L12Rik; Top-2 |
| Vector | pCMV6-AC-Myc-DDK |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR212050 representing NM_009409
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAAGTCCAGCCTCGCGGGCTCTGACGGGGCGCTGACCTGGGTGAACAATGCTACAAAAAAGGAAG AGTTGGAGACAGCAAACAAAAATGACTCTACTAAGAAGTTGTCTGTTGAGAGAGTGTACCAGAAGAAGAC CCAACTGGAGCACATTCTCCTTCGTCCTGATACATACATTGGATCAGTGGAGCCACTGACACAGCTAATG TGGGTGTATGATGAAGATGTAGGGATGAACTGCAGGGAGGTCACCTTTGTTCCAGGCTTATACAAGATCT TTGATGAAATTTTGGTTAATGCTGCTGACAACAAGCAGAGGGACAAGAACATGACCTGTATTAAGGTTTC TATTGATCCTGAATCTAATATTATAAGCATTTGGAATAATGGGAAAGGCATTCCAGTAGTAGAACACAAA GTAGAGAAAGTTTATGTTCCTGCTTTAATTTTTGGACAGCTTTTAACATCGAGTAATTATGACGATGATG AGAAAAAAGTTACAGGAGGCCGTAATGGTTATGGTGCAAAGCTTTGTAATATTTTTAGTACAAAGTTTAC AGTAGAAACGGCTTGCAAAGAATACAAACATAGTTTTAAACAGACATGGATGAATAATATGATGAAGACT TCTGAAGCCAAAATTAAACATTTTGATGGTGAAGATTATACATGCATAACATTCCAGCCAGATCTATCCA AATTTAAGATGGAAAAACTTGACAAGGACATTGTGGCCCTCATGACCAGAAGAGCATATGATTTAGCTGG GTCCTGTAAAGGAGTCAAGGTCATGTTTAATGGGAAGAAGTTGCCTGTAAATGGATTTCGCAGCTATGTA GACCTTTATGTGAAAGACAAATTGGATGAAACTGGGGTGGCTCTCAAAGTTATTCATGAACTTGCAAATG AAAGATGGGATGTTTGTCTAACCTTGAGTGAAAAAGGTTTCCAGCAGATCAGCTTTGTGAATAGTATTGC AACTACAAAAGGTGGGCGGCATGTGGATTATGTAGTAGATCAAGTTGTTAGTAAACTGATTGAAGTAGTT AAGAAAAAGAACAAAGCTGGTGTATCAGTGAAACCATTTCAGGTTAAAAACCATATATGGGTTTTTATTA ATTGTCTTATTGAAAATCCAACCTTTGATTCTCAAACTAAGGAAAACATGACTCTGCAGCCCAAAAGTTT TGGGTCCAAATGCCAACTGTCAGAAAAATTTTTTAAAGCAGCCTCTAATTGTGGCATTGTAGAAAGCATA TTGAACTGGGTGAAATTTAAGGCTCAGACTCAACTGAATAAGAAGTGTTCATCAGTAAAGTACAGTAAAA TCAAAGGCATTCCCAAACTGGATGATGCCAATGATGCTGGTGGCAAACATTCCCTGGAGTGTACATTAAT ATTAACAGAGGGGGACTCCGCCAAATCTCTAGCTGTATCTGGATTAGGTGTGATTGGTAGGGACAGATAT GGAGTCTTCCCACTCAGGGGTAAAATTCTCAATGTACGAGAAGCCTCTCATAAACAGATTATGGAAAATG CAGAGATAAATAATATTATTAAAATAGTTGGTTTGCAATACAAGAAAAGTTATGATGATGCAGAATCTCT GAAAACTTTACGTTATGGAAAGATTATGATTATGACTGATCAGGATCAAGATGGTTCTCACATCAAAGGC CTGCTAATCAATTTTATTCATCACAATTGGCCATCACTTTTGAAGCATGGTTTTCTTGAAGAATTCATTA CTCCCATTGTAAAGGCAAGCAAAAATAAGCAGGAACTTTCCTTTTATAGTATTCCTGAATTTGATGAGTG GAAAAAACATATAGAAAACCAGAAAGCCTGGAAAATAAAGTACTACAAAGGATTAGGTACCAGTACCGCT AAAGAAGCAAAGGAATATTTTGCTGATATGGAAAGGCATCGCATTTTGTTTAGATATGCTGGACCTGAAG ATGATGCTGCCATCACCTTGGCATTTAGTAAGAAGAAGATTGATGACAGAAAAGAGTGGTTAACCAACTT TATGGAGGATCGGAGACAGCGTAGGTTACATGGCCTACCAGAGCAATTTTTATATGGTACAGCAACAAAG CATTTGACATACAATGATTTCATCAACAAGGAATTGATTCTCTTCTCAAACTCGGACAATGAGAGATCTA TCCCATCTCTTGTGGATGGCTTCAAGCCAGGTCAACGAAAAGTTTTGTTTACCTGTTTCAAGAGGAATGA TAAACGTGAAGTGAAAGTAGCGCAGCTGGCTGGATCTGTTGCTGAGATGTCTGCTTACCACCATGGAGAA CAAGCATTGATGATGACTATTGTGAATTTGGCTCAGAATTTTGTGGGCAGTAATAACATTAACTTGCTTC AGCCCATTGGTCAGTTTGGAACTCGGCTTCATGGTGGCAAAGATGCTGCAAGCCCTCGTTATATTTTCAC AATGTTGAGCTCTCTGGCAAGACTTCTTTTTCCAGCTGTGGATGATAACTTGCTTAAGTTCCTTTATGAT GATAATCAACGTGTAGAGCCAGAGTGGTATATCCCTATAATCCCCATGGTTTTGATTAATGGTGCTGAGG GTATTGGGACTGGATGGGCTTGTAAACTGCCTAACTATGATGCTAGAGAAATTGTGAACAATGTCAGGCG GATGCTAGAAGGTTTGGATCCTCATCCCATGCTTCCAAACTATAAGAACTTCAAAGGCACCATCCAGGAA CTTGGTCAGAACCAGTATGCTGTTAGTGGTGAGATATTTGTGGTGGACAGAAACACAGTAGAGATTACAG AACTTCCAGTTAGAACATGGACACAGGTATACAAGGAGCAGGTCCTAGAACCTATGCTTAATGGAACAGA TAAAACACCGGCACTGATTTCTGATTACAAAGAATATCATACTGACACAACTGTGAAGTTTGTGGTGAAA ATGACAGAAGAGAAACTAGCGCAAGCAGAAGCTGCTGGCCTGCATAAAGTTTTTAAACTTCAAACTACTC TTACTTGCAATTCCATGGTACTCTTTGATCATATGGGTTGTCTGAAGAAGTATGAAACTGTGCAAGACAT TTTGAAAGAATTCTTTGATTTACGGTTAAGTTATTATGGTTTACGGAAGGAATGGCTTGTAGGAATGCTG GGAGCAGAATCTACAAAGCTTAATAATCAAGCCCGTTTCATTTTAGAGAAGATACAGGGGAAAATTACTA TAGAGAATAGGTCAAAGAAAGATTTGATTCAAATGTTAGTCCAGAGAGGTTATGAATCTGACCCAGTGAA AGCGTGGAAAGAAGCACAAGAAAAGGCTGCAGAAGAGGAAGATTCACAAAACCAACATGATGACAGTTCC TCTGATTCAGGAACTCCTTCAGGCCCTGATTTTAATTATATTTTAAATATGTCTCTATGGTCTCTTACTA AAGAAAAAGTTGAAGAACTCATTAAACAGAGAGATACGAAAGGGCGAGAGGTTAATGATCTCAAGAGGAA GTCTCCTTCAGATCTTTGGAAAGAAGACTTAGCAGCATTTGTTGAAGAATTGGATAAAGTGGAAGCACAA GAGCGGGAAGATATTCTGGCAGGAATGTCTGGGAAAGCAATAAAAGGTAAAGTCGGCAAACCCAAAGTAA AGAAACTTCAGCTGGAGGAGACAATGCCCTCGCCTTACGGGAGAAGAATTGTTCCAGAGATTACTGCTAT GAAGGCAGATGCCAGCAGAAAATTGCTGAAGAAGAAAAAGGGTGATCCTGATACCACAGTAGTGAAAGTA GAATTTGATGAAGAATTTAGTGGAACACCAGCAGAAGGTACAGGAGAGGAGACATTGACCCCATCTGCTC CTGTAAATAAAGGCCCCAAACCCAAAAGAGAGAAGAAGGAGCCTGGTACCAGGGTTAGAAAAACACCTAC ATCAACTGGTAAAACTAATGCGAAGAAAGTGAAGAAACGGAATCCTTGGTCAGATGATGAGTCCAAATCA GAGAGTGACTTGGAAGAAGCAGAACCTGTGGTTATCCCAAGAGATTCTTTGCTTAGGAGAGCAGCTGCTG AAAGACCTAAGTACACTTTTGATTTCTCAGAAGAGGAGGATGATGATGCTGCTGCTGCTGATGACAGTAA TGATTTAGAAGAACTGAAAGTTAAAGCATCTCCCATAACAAATGATGGGGAAGATGAATTTGTTCCTTCA GATGGCTTAGATAAAGATGAGTATGCTTTTTCTTCAGGCAAATCCAAAGCTACTCCAGAAAAATCTTCAA ATGACAAAAAAAGTCAGGATTTTGGAAATCTTTTTTCATTTCCTTCATATTCTCAGAAGTCAGAAGATGA TTCAGCTAAATTTGACAGTAATGAAGAAGATACCGCTTCTGTTTTTGCTCCATCATTTGGTCTGAAGCAA ACAGATAAACTTCCAAGTAAAACAGTTGCTGCTAAAAAGGGTAAACCACCTTCAGATACAGCCCCTAAAG CTAAGAGAGCCCCTAAACAGAAGAAGATAGTAGAGACTATAAACTCTGACTCAGACTCTGAATTTGGCAT TCCAAAGAAGACTACAACACCAAAAGGTAAAGGCCGAGGGGCAAAGAAAAGGAAAGCATCAGGCTCAGAA AATGAAGGTGATTATAACCCTGGAAGGAAACCATCTAAAACAGCCAGCAAGAAACCGAAGAAAACATCTT TTGATCAGGATTCAGATGTAGACATCTTCCCCTCAGACTTCACTTCTGAACCACCGGCTCTCCCACGGAC TGGTCGGGCTAGGAAAGAAGTAAAATATTTTGCAGAGTCTGATGAAGAAGAAGATGTTGATTTTGCAATG TTTAAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR212050 representing NM_009409
Red=Cloning site Green=Tags(s) MAKSSLAGSDGALTWVNNATKKEELETANKNDSTKKLSVERVYQKKTQLEHILLRPDTYIGSVEPLTQLM WVYDEDVGMNCREVTFVPGLYKIFDEILVNAADNKQRDKNMTCIKVSIDPESNIISIWNNGKGIPVVEHK VEKVYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWMNNMMKT SEAKIKHFDGEDYTCITFQPDLSKFKMEKLDKDIVALMTRRAYDLAGSCKGVKVMFNGKKLPVNGFRSYV DLYVKDKLDETGVALKVIHELANERWDVCLTLSEKGFQQISFVNSIATTKGGRHVDYVVDQVVSKLIEVV KKKNKAGVSVKPFQVKNHIWVFINCLIENPTFDSQTKENMTLQPKSFGSKCQLSEKFFKAASNCGIVESI LNWVKFKAQTQLNKKCSSVKYSKIKGIPKLDDANDAGGKHSLECTLILTEGDSAKSLAVSGLGVIGRDRY GVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYDDAESLKTLRYGKIMIMTDQDQDGSHIKG LLINFIHHNWPSLLKHGFLEEFITPIVKASKNKQELSFYSIPEFDEWKKHIENQKAWKIKYYKGLGTSTA KEAKEYFADMERHRILFRYAGPEDDAAITLAFSKKKIDDRKEWLTNFMEDRRQRRLHGLPEQFLYGTATK HLTYNDFINKELILFSNSDNERSIPSLVDGFKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGE QALMMTIVNLAQNFVGSNNINLLQPIGQFGTRLHGGKDAASPRYIFTMLSSLARLLFPAVDDNLLKFLYD DNQRVEPEWYIPIIPMVLINGAEGIGTGWACKLPNYDAREIVNNVRRMLEGLDPHPMLPNYKNFKGTIQE LGQNQYAVSGEIFVVDRNTVEITELPVRTWTQVYKEQVLEPMLNGTDKTPALISDYKEYHTDTTVKFVVK MTEEKLAQAEAAGLHKVFKLQTTLTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLSYYGLRKEWLVGML GAESTKLNNQARFILEKIQGKITIENRSKKDLIQMLVQRGYESDPVKAWKEAQEKAAEEEDSQNQHDDSS SDSGTPSGPDFNYILNMSLWSLTKEKVEELIKQRDTKGREVNDLKRKSPSDLWKEDLAAFVEELDKVEAQ EREDILAGMSGKAIKGKVGKPKVKKLQLEETMPSPYGRRIVPEITAMKADASRKLLKKKKGDPDTTVVKV EFDEEFSGTPAEGTGEETLTPSAPVNKGPKPKREKKEPGTRVRKTPTSTGKTNAKKVKKRNPWSDDESKS ESDLEEAEPVVIPRDSLLRRAAAERPKYTFDFSEEEDDDAAAADDSNDLEELKVKASPITNDGEDEFVPS DGLDKDEYAFSSGKSKATPEKSSNDKKSQDFGNLFSFPSYSQKSEDDSAKFDSNEEDTASVFAPSFGLKQ TDKLPSKTVAAKKGKPPSDTAPKAKRAPKQKKIVETINSDSDSEFGIPKKTTTPKGKGRGAKKRKASGSE NEGDYNPGRKPSKTASKKPKKTSFDQDSDVDIFPSDFTSEPPALPRTGRARKEVKYFAESDEEEDVDFAM FN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | NM_009409 |
| ORF Size | 4836 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009409.1, NM_009409.2, NP_033435.2 |
| RefSeq Size | 5652 bp |
| RefSeq ORF | 4839 bp |
| Locus ID | 21974 |
| UniProt ID | Q64511 |
| MW | 182.4 kDa |
| Gene Summary | Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224750 | Top2b (untagged) - Mouse topoisomerase (DNA) II beta (Top2b), (10ug) |
CNY 20,710.00 |
|
| MG212050 | Top2b (tGFP-tagged) - Mouse topoisomerase (DNA) II beta (Top2b) |
CNY 17,670.00 |
|
| MR212050L3 | Lenti ORF clone of Top2b (Myc-DDK-tagged) - Mouse topoisomerase (DNA) II beta (Top2b) |
CNY 17,960.00 |
|
| MR212050L4 | Lenti ORF clone of Top2b (mGFP-tagged) - Mouse topoisomerase (DNA) II beta (Top2b) |
CNY 17,960.00 |
