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Acly (NM_134037) Mouse Tagged ORF Clone
CAT＃: MR211618
- TrueORF®
Acly (Myc-DDK-tagged) - Mouse ATP citrate lyase (Acly), transcript variant 2
ORF Plasmid: tGFP
"NM_134037" in other vectors (4)
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CNY 7,600.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | A730098H14Rik; AW538652 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211618 representing NM_134037
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCAGCCAAGGCAATTTCAGAGCAGACCGGCAAAGAACTCCTGTACAAGTACATCTGCACCACCTCGG CCATCCAGAACCGGTTCAAGTACGCCCGGGTGACTCCCGACACAGACTGGGCCCATCTGCTGCAGGACCA CCCGTGGCTGCTCAGCCAGAGCTTGGTTGTCAAGCCAGACCAGTTAATCAAACGTCGAGGAAAGCTTGGC CTCGTCGGGGTCAATCTCTCTCTGGATGGAGTCAAATCCTGGCTAAAACCTCGCCTGGGACATGAAGCCA CTGTCGGCAAGGCCAAAGGCTTCCTCAAGAACTTTCTCATTGAACCCTTCGTCCCCCACAGTCAGGCGGA GGAGTTCTACGTGTGCATCTATGCTACCCGGGAAGGAGACTACGTCCTGTTCCACCATGAAGGGGGTGTG GATGTGGGCGATGTGGATGCCAAAGCCCAGAAGCTGCTTGTGGGTGTGGACGAAAAGCTGAATACCGAGG ACATTAAGAGACACCTGTTGGTCCATGCACCTGAGGACAAGAAAGAAGTCCTGGCCAGCTTCATCTCTGG TCTATTCAATTTCTACGAGGATCTGTACTTCACCTACCTTGAGATCAACCCCCTTGTGGTGACCAAAGAT GGTGTCTACATCCTTGACTTGGCGGCCAAGGTGGATGCCACAGCTGACTACATCTGTAAAGTCAAGTGGG GTGATATAGAGTTCCCTCCCCCCTTTGGGCGTGAGGCGTACCCCGAGGAAGCCTACATTGCAGACCTGGA TGCCAAAAGTGGAGCAAGCTTGAAGCTGACCTTGCTGAACCCCAAGGGGCGGATCTGGACCATGGTTGCT GGGGGTGGCGCCTCCGTCGTGTACAGTGACACCATCTGTGATCTTGGAGGTGTCAATGAACTGGCGAATT ACGGGGAATACTCGGGTGCCCCCAGTGAACAACAGACCTATGACTATGCCAAGACCATCCTCTCACTTAT GACTCGAGAGAAGCACCCAGAAGGCAAGATCCTCATCATTGGAGGCAGCATTGCAAACTTCACCAATGTG GCCGCCACCTTCAAGGGCATTGTGAGAGCGATTCGAGATTACCAGGGTCCCCTGAAGGAGCATGAGGTCA CCATCTTTGTCCGAAGAGGTGGCCCCAACTATCAAGAGGGATTACGAGTGATGGGAGAAGTTGGGAAGAC CACTGGGATCCCCATCCATGTCTTTGGCACAGAAACTCACATGACGGCCATTGTGGGCATGGCCCTGGGC CACCGGCCCATTCCCAACCAGCCACCCACAGCAGCTCACACTGCCAACTTCCTCCTTAATGCCAGCGGGA GCACATCGACTCCAGCACCCAGTAGGACAGCATCTTTTTCTGAGTCCCGAGCTGATGAAGTGGCACCTGC AAAGAAAGCCAAGCCAGCTATGCCCCAAGGAAAGAGTGCCACCCTCTTCAGCCGACATACCAAGGCCATC GTGTGGGGCATGCAGACCCGGGCTGTGCAGGGCATGCTGGACTTTGACTACGTGTGTTCCCGAGACGAGC CCTCAGTGGCTGCCATGGTCTACCCTTTCACTGGGGATCACAAGCAGAAGTTTTACTGGGGACACAAGGA AATCCTGATCCCTGTCTTCAAGAACATGGCTGACGCCATGAAGAAGCACCCGGAGGTAGACGTGCTGATC AACTTTGCGTCTCTGCGGTCCGCTTACGACAGCACCATGGAGACCATGAACTATGCCCAGATCCGCACCA TAGCCATCATAGCAGAAGGTATCCCTGAGGCCCTCACACGGAAGCTCATCAAGAAGGCCGACCAGAAGGG AGTGACCATCATTGGGCCAGCTACGGTTGGGGGCATTAAGCCTGGATGCTTTAAGATCGGGAATACTGGT GGAATGCTGGACAACATCCTGGCCTCCAAACTGTACCGCCCAGGCAGCGTGGCCTACGTCTCACGTTCAG GAGGCATGTCTAATGAACTCAATAACATCATCTCTCGGACCACAGATGGTGTCTATGAGGGCGTGGCCAT CGGCGGGGACAGGTACCCTGGGTCCACATTCATGGATCACGTGCTGCGCTACCAGGACACTCCAGGAGTC AAGATGATCGTAGTTCTTGGGGAGATAGGGGGCACAGAGGAATATAAGATCTGCCGGGGCATCAAGGAGG GCCGCCTCACCAAGCCAGTGGTCTGCTGGTGTATCGGGACCTGTGCCACCATGTTCTCCTCCGAGGTCCA GTTTGGCCATGCTGGAGCTTGTGCCAACCAGGCTTCTGAAACTGCAGTAGCCAAGAACCAGGCCTTGAAG GAAGCAGGAGTGTTTGTGCCCCGAAGCTTCGATGAGCTTGGAGAAATCATTCAGTCTGTGTATGAAGATC TGGTGGCCAAAGGAGCCATTGTACCTGCCCAGGAAGTGCCACCTCCAACAGTGCCCATGGACTACTCTTG GGCCAGAGAGCTGGGTTTGATCCGAAAACCTGCCTCATTCATGACCAGCATCTGTGATGAGCGAGGGCAG GAGCTCATTTATGCGGGCATGCCCATCACCGAGGTCTTCAAGGAGGAGATGGGCATCGGTGGTGTCCTCG GCCTCCTCTGGTTCCAGAGAAGGTTGCCCAAGTATTCCTGCCAGTTCATTGAGATGTGTCTGATGGTCAC AGCTGATCACGGGCCAGCTGTCTCTGGAGCCCATAACACCATCATCTGTGCTCGGGCTGGGAAGGACCTG GTCTCCAGCCTCACCTCAGGGCTGCTCACCATTGGAGACCGGTTTGGGGGTGCCTTGGATGCCGCAGCAA AGATGTTCAGTAAAGCCTTTGACAGCGGCATCATTCCCATGGAGTTCGTCAACAAGATGAAGAAGGAGGG GAAGCTGATCATGGGCATCGGCCATCGAGTAAAATCGATAAACAACCCAGACATGCGAGTGCAGATCCTC AAGGACTTCGTCAAACAGCACTTCCCCGCCACCCCGCTGCTCGACTATGCCCTGGAAGTGGAGAAGATTA CCACCTCCAAGAAGCCAAATCTTATCCTGAATGTGGACGGCTTCATCGGCGTTGCGTTTGTGGACATGCT CAGGAACTGTGGCTCCTTCACCCGGGAGGAAGCTGATGAATATGTTGACATTGGAGCCCTCAATGGCATC TTTGTGCTAGGAAGGAGTATGGGCTTCATTGGGCACTACCTTGACCAGAAGAGGCTGAAGCAAGGGCTGT ATCGTCACCCCTGGGATGACATTTCCTATGTTCTTCCAGAACACATGAGCATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211618 representing NM_134037
Red=Cloning site Green=Tags(s) MSAKAISEQTGKELLYKYICTTSAIQNRFKYARVTPDTDWAHLLQDHPWLLSQSLVVKPDQLIKRRGKLG LVGVNLSLDGVKSWLKPRLGHEATVGKAKGFLKNFLIEPFVPHSQAEEFYVCIYATREGDYVLFHHEGGV DVGDVDAKAQKLLVGVDEKLNTEDIKRHLLVHAPEDKKEVLASFISGLFNFYEDLYFTYLEINPLVVTKD GVYILDLAAKVDATADYICKVKWGDIEFPPPFGREAYPEEAYIADLDAKSGASLKLTLLNPKGRIWTMVA GGGASVVYSDTICDLGGVNELANYGEYSGAPSEQQTYDYAKTILSLMTREKHPEGKILIIGGSIANFTNV AATFKGIVRAIRDYQGPLKEHEVTIFVRRGGPNYQEGLRVMGEVGKTTGIPIHVFGTETHMTAIVGMALG HRPIPNQPPTAAHTANFLLNASGSTSTPAPSRTASFSESRADEVAPAKKAKPAMPQGKSATLFSRHTKAI VWGMQTRAVQGMLDFDYVCSRDEPSVAAMVYPFTGDHKQKFYWGHKEILIPVFKNMADAMKKHPEVDVLI NFASLRSAYDSTMETMNYAQIRTIAIIAEGIPEALTRKLIKKADQKGVTIIGPATVGGIKPGCFKIGNTG GMLDNILASKLYRPGSVAYVSRSGGMSNELNNIISRTTDGVYEGVAIGGDRYPGSTFMDHVLRYQDTPGV KMIVVLGEIGGTEEYKICRGIKEGRLTKPVVCWCIGTCATMFSSEVQFGHAGACANQASETAVAKNQALK EAGVFVPRSFDELGEIIQSVYEDLVAKGAIVPAQEVPPPTVPMDYSWARELGLIRKPASFMTSICDERGQ ELIYAGMPITEVFKEEMGIGGVLGLLWFQRRLPKYSCQFIEMCLMVTADHGPAVSGAHNTIICARAGKDL VSSLTSGLLTIGDRFGGALDAAAKMFSKAFDSGIIPMEFVNKMKKEGKLIMGIGHRVKSINNPDMRVQIL KDFVKQHFPATPLLDYALEVEKITTSKKPNLILNVDGFIGVAFVDMLRNCGSFTREEADEYVDIGALNGI FVLGRSMGFIGHYLDQKRLKQGLYRHPWDDISYVLPEHMSM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_134037 |
| ORF Size | 3273 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_134037.3, NP_598798.1 |
| RefSeq Size | 4408 bp |
| RefSeq ORF | 3276 bp |
| Locus ID | 104112 |
| UniProt ID | Q91V92 |
| MW | 120.2 kDa |
| Gene Summary | ATP-citrate synthase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis (PubMed:25450640). In nervous tissue it may be involved in the biosynthesis of acetylcholine (By similarity) (PubMed:25450640).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC205217 | Acly (untagged) - Mouse ATP citrate lyase (Acly), transcript variant 2, (10ug) |
CNY 7,608.00 |
|
| MG211618 | Acly (tGFP-tagged) - Mouse ATP citrate lyase (Acly) |
CNY 9,200.00 |
|
| MR211618L3 | Lenti ORF clone of Acly (Myc-DDK-tagged) - Mouse ATP citrate lyase (Acly), transcript variant 2 |
CNY 11,500.00 |
|
| MR211618L4 | Lenti ORF clone of Acly (mGFP-tagged) - Mouse ATP citrate lyase (Acly), transcript variant 2 |
CNY 11,500.00 |
