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Gldc (NM_138595) Mouse Tagged ORF Clone
CAT＃: MR211473
- TrueORF®
Gldc (Myc-DDK-tagged) - Mouse glycine decarboxylase (Gldc), nuclear gene encoding mitochondrial protein
ORF Plasmid: tGFP
"NM_138595" in other vectors (4)
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CNY 7,136.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | b2b2679Clo; D030049L12Rik; D19Wsu57e |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211473 representing NM_138595
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGCTGTGTGCCAGGGCATGGGGGCTGCGCCTGGGCCGCGGAGCCGGGGGCGGCCACCGCCTGGCTC GGGGCACCGGCTTGAGTTGGGCGCAGCGAAGCCGGGACAGCAGCGGCGGCGGTGGCGGTGGCGGTGGCGG GGACCGCGGCGCGGCTGGGGCCTCGCGCCTTCTGGAGCGCCTCCTGCCCAGACACGATGACTTCTCCCGG AGACACATCGGCCCCGGAGACAAAGACCGGAGGGAGATGCTGCAGGCGCTGGGGCTGGCGAGCATTGATG AGCTCATCGAGAAGACGGTCCCCGCCAGCATCCGCTTGAAGAGACCCTTGAAAATGGAAGACCCTATTTG TGAGAATGAAATCCTCGAGACTCTGCATGCCATTGCAAGCAAGAACCAGATCTGGAGGTCGTATATTGGC ATGGGCTATTACAACTGCTCGGTGCCCCAGACCATTTTGCGGAACTTACTGGAGAATTCTGGATGGGTCA CCCAGTACACTCCGTATCAGCCAGAGGTGTCCCAGGGGAGGCTGGAGAGTTTACTCAACTACCAGACCAT GGTGTCTGACATCACAGGCTTGGACATGGCCAACGCTTCCCTGCTGGATGAGGCAACCGCGGCTGCAGAG GCGATGCAACTGTGCCACAGACACAACAAGAGGAAGAAATTTTTCGTTGATCCTCGTTGTCACCCACAGA CGATAGCTGTTGTCCAGACTCGAGCCAAATATAGGGGGGTGCTTGTCGAGCTGAAGTTACCCCACGAAAT GGATTTCAGTGGCAAAGACGTCTGTGGGGTGCTGTTCCAGTACCCAGACACTGAGGGGAAGGTGGAGGAC TTCACAGAACTTGTAGACAGAGCCCATCAGACTGGGAGCCTGACCTGCTGTGCTACTGACCTTTTAGCTC TGTGTATTTTGAGGCCACCTGGAGAGTTTGGAGTGGACATTGCCCTGGGCAACTCCCAGAGATTTGGAGT GCCACTGGGCTATGGGGGACCGCATGCGGCCTTCTTTGCTGTCAAAGAAAACCTGGTGAGGATGATGCCT GGAAGGATGGTGGGGGTGACAAGGGATGCCACAGGGAAGGAAGTGTATCGCCTCGCTCTCCAGACCAGAG AACAACACATCCGGAGAGACAAAGCCACCAGCAACATCTGCACGGCTCAGGCCCTTTTAGCCAACATGGC TGCCATGTTCGCCATTTACCATGGATCCCAGGGGCTGAAGCACATTGCTAAAAGGGTTCATAATGCTACT TTGATTTTGTCTGAAGGGCTCAAGCGAGCAGGACACCAACTCCAGCATGACCTGTTCTTTGACACTTTGA AGGTTCAGTGTGGCTGCTCAGTGAAGGAGGTGCTGGGCAGAGCAGCTCAGAGGCAAATCAACTTTCGACT CTTCGATGACGGCACACTTGGCATTTCTTTGGATGAAACAGTCACTGAGAAAGATCTGGATGATTTACTG TGGATCTTCGGCTGTGAGTCATCTGCAGAATTAGTTGCTGAAGGCATGGGAGAGGAGCGGAGAGGTCTTC TAGGGTCTTCATTCAAGAGAACCAGCCCGTTCCTCACTCATCAGGTGTTCAACAGCTATCACTCAGAAAC TAATCTTGTCCGGTATATGAAGAAACTGGAGAACAAAGACATCTCCCTCGTTCACAGCATGATCCCACTG GGATCCTGCACCATGAAGCTCAATAGCTCCTCCGAACTTGCACCCATCACCTGGAGAGAATTTGCCAACA TCCACCCCTTTGTGCCGCTGGACCAAGCCCAGGGATACCAACAGCTTTTCCAAGGGCTGGAGAAGGATCT GTGTGAGATCACAGGCTACGACCGAGTCTCATTCCAGCCAAACAGCGGAGCGCAGGGGGAGTACGCCGGA CTGGCCACCATCAGAGCGTACTTAGACCAGAAAGGAGAGAGACACAGAACAGTCTGTCTCATTCCTAAGT CAGCACATGGAACGAATCCAGCAAGCGCTCACATGGCGGGCATGAAGATCCAGCCTGTGGAGGTGGACAG ATACGGGAACATCGATGTGGCTCACCTCAAGGCCATGGTGGACCAACACAAGGAGAACCTGGCCGCCATC ATGATCACGTATCCATCTACCAATGGTGTATTTGAAGAGAATATCGGCGATGTGTGCGCCCTGATTCACC AACACGGAGGACAGGTCTACCTCGACGGGGCGAACATGAATGCCCAGGTGGGAATCTGCCGCCCTGGGGA CTTTGGGTCAGACGTCTCTCACCTAAATCTTCACAAGACCTTCTGCATTCCCCACGGAGGGGGCGGTCCT GGCATGGGGCCCATCGGAGTGAAGAAGCACCTCAGCCCCTTTCTGCCCAGCCACCCCGTCATTTCCATAA AGCCAACTGAAGGCACCTGGCCCGTGGGCACTGTCAGTGCAGCCCCGTGGGGTTCCAGCTCCATCCTGCC CATTTCATGGGCTTACATTAAGATGATGGGTGGAAAGGGCCTCAAAGAAGCCACAGAAATCGCCATTCTA AATGCCAACTACATGGCCAAACGACTAGAGAAACACTACAGAGTCCTCTTTAGAGGTGCAAGAGGGTATG TGGCTCATGAGTTTATCTTGGACACCCGACCCTTCAAAAAGTCTGCCAATGTTGAGGCTGTGGATGTTGC CAAGAGGCTCCAGGATTATGGATTTCACGCCCCTACCATGTCCTGGCCTGTGGCAGGGACTCTCATGATT GAGCCCACCGAGTCAGAAGACAAGGCAGAGCTCGACAGATTCTGTGATGCTATGATCAGCATCAGGCAAG AAATCGCTGACATAGAGGAGGGCCGCATCGACCCGAGGGTCAACCCCCTGAAGATGTCTCCACACTCCTT GACCTGTGTCACATCCTCCTGCTGGGATCGGCCGTATTCTAGAGAGGTAGCAGCATTTCCACTGCCCTTT GTGAAACCAGAGAACAAATTCTGGCCAACCATTGCCCGGATCGATGACATCTACGGAGATCAGCACTTGG TCTGCACCTGCCCGCCCATGGAGGTCTATGAGTCTCCATTTTCTGAACAGAAGAGGGCTTCTTCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211473 representing NM_138595
Red=Cloning site Green=Tags(s) MQLCARAWGLRLGRGAGGGHRLARGTGLSWAQRSRDSSGGGGGGGGGDRGAAGASRLLERLLPRHDDFSR RHIGPGDKDRREMLQALGLASIDELIEKTVPASIRLKRPLKMEDPICENEILETLHAIASKNQIWRSYIG MGYYNCSVPQTILRNLLENSGWVTQYTPYQPEVSQGRLESLLNYQTMVSDITGLDMANASLLDEATAAAE AMQLCHRHNKRKKFFVDPRCHPQTIAVVQTRAKYRGVLVELKLPHEMDFSGKDVCGVLFQYPDTEGKVED FTELVDRAHQTGSLTCCATDLLALCILRPPGEFGVDIALGNSQRFGVPLGYGGPHAAFFAVKENLVRMMP GRMVGVTRDATGKEVYRLALQTREQHIRRDKATSNICTAQALLANMAAMFAIYHGSQGLKHIAKRVHNAT LILSEGLKRAGHQLQHDLFFDTLKVQCGCSVKEVLGRAAQRQINFRLFDDGTLGISLDETVTEKDLDDLL WIFGCESSAELVAEGMGEERRGLLGSSFKRTSPFLTHQVFNSYHSETNLVRYMKKLENKDISLVHSMIPL GSCTMKLNSSSELAPITWREFANIHPFVPLDQAQGYQQLFQGLEKDLCEITGYDRVSFQPNSGAQGEYAG LATIRAYLDQKGERHRTVCLIPKSAHGTNPASAHMAGMKIQPVEVDRYGNIDVAHLKAMVDQHKENLAAI MITYPSTNGVFEENIGDVCALIHQHGGQVYLDGANMNAQVGICRPGDFGSDVSHLNLHKTFCIPHGGGGP GMGPIGVKKHLSPFLPSHPVISIKPTEGTWPVGTVSAAPWGSSSILPISWAYIKMMGGKGLKEATEIAIL NANYMAKRLEKHYRVLFRGARGYVAHEFILDTRPFKKSANVEAVDVAKRLQDYGFHAPTMSWPVAGTLMI EPTESEDKAELDRFCDAMISIRQEIADIEEGRIDPRVNPLKMSPHSLTCVTSSCWDRPYSREVAAFPLPF VKPENKFWPTIARIDDIYGDQHLVCTCPPMEVYESPFSEQKRASS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_138595 |
| ORF Size | 3075 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_138595.2 |
| RefSeq Size | 3769 bp |
| RefSeq ORF | 3078 bp |
| Locus ID | 104174 |
| UniProt ID | Q91W43 |
| MW | 113.7 kDa |
| Gene Summary | The glycine cleavage system catalyzes the degradation of glycine. The P protein (GLDC) binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO(2) is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein (GCSH) (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC205351 | Gldc (untagged) - Mouse glycine decarboxylase (Gldc), nuclear gene encoding mitochondrial protein, (10ug) |
CNY 7,144.00 |
|
| MG211473 | Gldc (tGFP-tagged) - Mouse glycine decarboxylase (Gldc) |
CNY 9,790.00 |
|
| MR211473L3 | Lenti ORF clone of Gldc (Myc-DDK-tagged) - Mouse glycine decarboxylase (Gldc), nuclear gene encoding mitochondrial protein |
CNY 10,830.00 |
|
| MR211473L4 | Lenti ORF clone of Gldc (mGFP-tagged) - Mouse glycine decarboxylase (Gldc), nuclear gene encoding mitochondrial protein |
CNY 10,830.00 |
