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Pomt1 (NM_145145) Mouse Tagged ORF Clone
CAT＃: MR210437
- TrueORF®
Pomt1 (Myc-DDK-tagged) - Mouse protein-O-mannosyltransferase 1 (Pomt1)
ORF Plasmid: tGFP
"NM_145145" in other vectors (4)
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CNY 5,464.00
CNY 6,560.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AI505244 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210437 representing NM_145145
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGAGCCACTCTACGGGACTCGAAGAAACGCTCGGAGTCCTCCCGAGCTGGCTTTTCTGCAAAATGT TAAGATTTTTGAAACGGCCTCTAGTGGTGACTGTTGACATCAATTTGAACTTGGTAGCTCTGACTGGCCT GGGACTACTTACCCGACTATGGCAACTCTCCTACCCTCGGGCTGTGGTTTTCGATGAAGTATATTATGGG CAGTACATTTCCTTCTACATGAAGCGCATCTTCTTTCTGGATGACAGTGGGCCACCATTTGGCCACATGC TACTGGCCTTAGGAGGTTGGTTAGGGGGATTCGATGGTAACTTTCTGTGGAACCGAATTGGAGCAGAGTA CAGTAGCAATGTGCCTATATGGTCCTTACGCCTGCTGCCAGCGCTTGCCGGGGCCCTGTCAGTGCCCATG GCCTACCAGATAGTGCTAGAGCTCCACTTTTCCCACGGTGCTGCCATTGGAGCCGCCCTGCTGATGCTCA TTGAGAACGCCCTGATCACTCAGTCCAGGCTCATGCTGTTGGAGTCCATACTGATATTTTTTAACCTGTT GGCTGTGTTGTCCTATCTGAAGTTCTTCAACTCCCAGACACACAGCCCTTTCTCAGTGCACTGGTGGCTG TGGCTACTGCTGACCGGGGTCTCTTGTTCCTGTGCAGTTGGGATCAAATACATGGGGATTTTCACCTACC TGCTTGTGCTCGGCATTGCAGCTGTCCACGCGTGGAATCTGATCGGAGACCAGACCTTGTCAAATATGCG CGTGCTCAGTCACTTGCTCGCCAGAATCGTGGCTCTGCTGGTCGTCCCAGTCTTCCTGTACTTACTCTTC TTCTATGTCCACCTGATGCTGCTCTACCGCTCTGGGCCCCATGACCAAATCATGTCCAGTGCCTTCCAGG CCAGCTTGGAGGGAGGGCTTGCCCGCATCACCCAAGGCCAGCCCCTGGAGGTGGCCTTTGGTTCCCAGGT CACTCTGAAGAGCGTCTCTGGCAAACCCTTGCCCTGCTGGCTTCATTCGCACAAGAACACCTATCCCATG ATATATGAGAATGGCCGTGGCAGCTCCCACCAGCAACAGGTGACCTGTTATCCCTTCAAAGACATCAATA ACTGGTGGATCGTCAAGGATCCTGGGCGACACCAGCTGGTGGTAAACAACCCTCCCCGGCCTGTAAGACA TGGAGACATTGTACAGCTCGTTCACGGCATGACCACCCGCCTCCTTAACACGCACGATGTCGCAGCCCCA CTGAGCCCCCATTCTCAAGAAGTCTCCTGCTACATTGACTATAACATCTCCATGCCTGCCCAGAACCTCT GGAAACTGGACATTGTGAACAGAGAGTCCAACCGGGATACCTGGAAGACTATCTTGTCGGAAGTGCGCTT TGTACATGTGAACACATCCGCCATCTTGAAGCTGAGCGGGGCTCACCTCCCTGACTGGGGGTTCCGGCAG TTGGAGGTAGTTGGGGAGAAGTTGTCACCGGGCTACCACGAGAGCATGGTGTGGAATGTGGAAGAACACC GGTATGGCAAAAGCCATGAGCAGAAGGAGAGGGAGCTGGAGCTCCACTCGCCCACTCAGCTCGATATCAG CAGGAACCTCAGCTTCATGGCCAGATTCTCAGAGTTACAGTGGAAGATGCTGACGCTGAAGAATGAGGAC TTGGAACACCAGTACAGCTCCACCCCGCTGGAGTGGCTCACGCTGGACACCAACATCGCCTATTGGCTAC ATCCCAGGACCAGTGCTCAGATCCACTTGCTTGGGAACATTGTGATCTGGACTTCAGCCAGCCTTGCCAC AGTGGTCTACACTCTACTCTTCTTCTGGTACCTGCTCCGCCGGCGAAGGAGCATCTGTGACCTCCCTGAG GATGCCTGGTCCCGCTGGGTGCTGGCTGGAGCCCTGTGTACTGGCGGCTGGGCACTCAACTACCTGCCCT TCTTCCTGATGGAGAGGGTGCTCTTCCTCTACCACTACTTGCCGGCACTCACCTTCCAGATCCTGCTGCT CCCGATTGTCCTGCAGCACGCCAGCGACCATCTGTGCAGGTCCCAGCTGCAGAGGAATGTCTTCAGTGCC CTGGTTGTAGCATGGTATTCCTCCGCATGCCATGTGTCCAACATGCTACGCCCACTAACCTATGGGGACA CGTCACTCTCACCAGGCGAGCTCCGGGCCCTTCGCTGGAAAGACAGCTGGGATATTCTGATCCGAAAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >MR210437 representing NM_145145
Red=Cloning site Green=Tags(s) MGSHSTGLEETLGVLPSWLFCKMLRFLKRPLVVTVDINLNLVALTGLGLLTRLWQLSYPRAVVFDEVYYG QYISFYMKRIFFLDDSGPPFGHMLLALGGWLGGFDGNFLWNRIGAEYSSNVPIWSLRLLPALAGALSVPM AYQIVLELHFSHGAAIGAALLMLIENALITQSRLMLLESILIFFNLLAVLSYLKFFNSQTHSPFSVHWWL WLLLTGVSCSCAVGIKYMGIFTYLLVLGIAAVHAWNLIGDQTLSNMRVLSHLLARIVALLVVPVFLYLLF FYVHLMLLYRSGPHDQIMSSAFQASLEGGLARITQGQPLEVAFGSQVTLKSVSGKPLPCWLHSHKNTYPM IYENGRGSSHQQQVTCYPFKDINNWWIVKDPGRHQLVVNNPPRPVRHGDIVQLVHGMTTRLLNTHDVAAP LSPHSQEVSCYIDYNISMPAQNLWKLDIVNRESNRDTWKTILSEVRFVHVNTSAILKLSGAHLPDWGFRQ LEVVGEKLSPGYHESMVWNVEEHRYGKSHEQKERELELHSPTQLDISRNLSFMARFSELQWKMLTLKNED LEHQYSSTPLEWLTLDTNIAYWLHPRTSAQIHLLGNIVIWTSASLATVVYTLLFFWYLLRRRRSICDLPE DAWSRWVLAGALCTGGWALNYLPFFLMERVLFLYHYLPALTFQILLLPIVLQHASDHLCRSQLQRNVFSA LVVAWYSSACHVSNMLRPLTYGDTSLSPGELRALRWKDSWDILIRK SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_145145 |
| ORF Size | 2238 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_145145.2 |
| RefSeq Size | 2838 bp |
| RefSeq ORF | 2241 bp |
| Locus ID | 99011 |
| UniProt ID | Q8R2R1 |
| MW | 85.7 kDa |
| Gene Summary | Transfers mannosyl residues to the hydroxyl group of serine or threonine residues. Coexpression of both POMT1 and POMT2 is necessary for enzyme activity, expression of either POMT1 or POMT2 alone is insufficient. Essentially dedicated to O-mannosylation of alpha-DAG1 and few other proteins but not of cadherins and protocaherins.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221317 | Pomt1 (untagged) - Mouse protein-O-mannosyltransferase 1 (Pomt1), (10ug) |
CNY 9,690.00 |
|
| MG210437 | Pomt1 (tGFP-tagged) - Mouse protein-O-mannosyltransferase 1 (Pomt1) |
CNY 7,220.00 |
|
| MR210437L3 | Lenti ORF clone of Pomt1 (Myc-DDK-tagged) - Mouse protein-O-mannosyltransferase 1 (Pomt1) |
CNY 8,460.00 |
|
| MR210437L4 | Lenti ORF clone of Pomt1 (mGFP-tagged) - Mouse protein-O-mannosyltransferase 1 (Pomt1) |
CNY 8,460.00 |
