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Cyp2e1 (NM_021282) Mouse Tagged ORF Clone
CAT＃: MR207907
- TrueORF®
Cyp2e1 (Myc-DDK-tagged) - Mouse cytochrome P450, family 2, subfamily e, polypeptide 1 (Cyp2e1)
ORF Plasmid: tGFP
"NM_021282" in other vectors (3)
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CNY 5,488.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Cyp2e |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR207907 representing NM_021282
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGTTCTTGGCATCACCGTTGCCTTGCTTGTCTGGATCGCCACCCTCCTCCTCGTATCCATCTGGA AACAGATCTATAGAAGTTGGAACCTGCCCCCAGGACCTTTCCCAATTCCTTTCTTTGGAAACATTTTTCA GCTGGATTTGAAGGATATTCCCAAGTCTTTAACCAAGTTGGCAAAGCGCTTCGGGCCAGTGTTCACACTG CACCTGGGTCAGAGGCGCATCGTGGTCCTGCATGGCTACAAGGCTGTCAAGGAGGTGCTACTGAACCACA AGAATGAGTTCTCTGGCCGAGGGGACATTCCTGTGTTCCAGGAGTACAAGAACAAGGGGATTATTTTCAA TAATGGACCCACATGGAAGGACGTGCGGAGGTTTTCCCTAAGTATCCTCCGTGACTGGGGAATGGGGAAA CAGGGTAATGAGGCCCGCATCCAAAGAGAGGCACACTTCCTGGTGGAGGAGCTCAAAAAGACCAAAGGCC AGCCTTTTGACCCTACCTTTCTGATTGGCTGTGCACCCTGCAATGTCATTGCGGATATTCTCTTCAACAA ACGCTTCGATTACGATGACAAGAAGTGTCTGGAGCTCATGAGTTTGTTCAATGAAAACTTCTACCTGCTG AGTACTCCCTGGATCCAGGCTTACAATTACTTTTCGGATTATCTACAATATCTACCTGGAAGCCACAGAA AAGTCATGAAAAATGTGTCTGAAATAAGACAGTACACACTTGGAAAAGCCAAGGAACACCTTAAGTCACT GGACATCAACTGCCCCCGGGATGTGACTGACTGTCTCCTCATAGAGATGGAGAAGGAAAAACACAGCCAA GAACCCATGTACACAATGGAAAATATTTCTGTGACTTTGGCCGACCTGTTCTTTGCAGGAACAGAGACCA CCAGCACAACTCTGAGATATGGGCTCCTGATTCTCATGAAATACCCAGAAATTGAAGAGAAACTTCATGA AGAAATTGACAGGGTTATTGGGCCAAGCCGTGCCCCTGCAGTCCGAGACAGGATGAATATGCCCTACATG GACGCTGTAGTGCATGAGATTCAGAGATTCATCAACCTCGTCCCTTCCAACCTGCCCCACGAAGCAACCC GAGACACCGTGTTCCGAGGATATGTCATCCCCAAGGGTACAGTTGTAATTCCAACTCTGGACTCCCTTTT ATTTGACAACTATGAGTTTCCAGATCCAGAGACATTTAAACCTGAGCATTTTCTGAATGAAAATGGGAAG TTCAAGTACAGTGACTATTTCAAGGCGTTTTCTGCAGGAAAGCGCGTGTGTGTTGGAGAAGGCCTGGCCC GCATGGAACTGTTTCTGCTTTTGTCTGCTATTCTGCAGCATTTTAATCTGAAGTCTCTGGTTGACCCTAA GGATATCGACCTCAGCCCTGTTACAATTGGCTTTGGCAGTATCCCACGCGAATTTAAACTCTGTGTCATT CCTCGTTCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR207907 representing NM_021282
Red=Cloning site Green=Tags(s) MAVLGITVALLVWIATLLLVSIWKQIYRSWNLPPGPFPIPFFGNIFQLDLKDIPKSLTKLAKRFGPVFTL HLGQRRIVVLHGYKAVKEVLLNHKNEFSGRGDIPVFQEYKNKGIIFNNGPTWKDVRRFSLSILRDWGMGK QGNEARIQREAHFLVEELKKTKGQPFDPTFLIGCAPCNVIADILFNKRFDYDDKKCLELMSLFNENFYLL STPWIQAYNYFSDYLQYLPGSHRKVMKNVSEIRQYTLGKAKEHLKSLDINCPRDVTDCLLIEMEKEKHSQ EPMYTMENISVTLADLFFAGTETTSTTLRYGLLILMKYPEIEEKLHEEIDRVIGPSRAPAVRDRMNMPYM DAVVHEIQRFINLVPSNLPHEATRDTVFRGYVIPKGTVVIPTLDSLLFDNYEFPDPETFKPEHFLNENGK FKYSDYFKAFSAGKRVCVGEGLARMELFLLLSAILQHFNLKSLVDPKDIDLSPVTIGFGSIPREFKLCVI PRS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_021282 |
| ORF Size | 1479 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_021282.3 |
| RefSeq Size | 1759 bp |
| RefSeq ORF | 1482 bp |
| Locus ID | 13106 |
| UniProt ID | Q05421 |
| MW | 57.3 kDa |
| Gene Summary | A cytochrome P450 monooxygenase involved in the metabolism of fatty acids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Hydroxylates fatty acids specifically at the omega-1 position displaying the highest catalytic activity for saturated fatty acids. May be involved in the oxidative metabolism of xenobiotics.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG207907 | Cyp2e1 (tGFP-tagged) - Mouse cytochrome P450, family 2, subfamily e, polypeptide 1 (Cyp2e1) |
CNY 7,088.00 |
|
| MR207907L3 | Lenti ORF clone of Cyp2e1 (Myc-DDK-tagged) - Mouse cytochrome P450, family 2, subfamily e, polypeptide 1 (Cyp2e1) |
CNY 6,840.00 |
|
| MR207907L4 | Lenti ORF clone of Cyp2e1 (mGFP-tagged) - Mouse cytochrome P450, family 2, subfamily e, polypeptide 1 (Cyp2e1) |
CNY 6,840.00 |
