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Cyp27b1 (NM_010009) Mouse Tagged ORF Clone
CAT＃: MR207155
- TrueORF®
Cyp27b1 (Myc-DDK-tagged) - Mouse cytochrome P450, family 27, subfamily b, polypeptide 1 (Cyp27b1), nuclear gene encoding mitochondrial protein
ORF Plasmid: tGFP
"NM_010009" in other vectors (4)
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CNY 5,488.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Cp2b; Cyp1; Cyp27b; Cyp40; P450c1; Pddr; Vdd1; Vddr; VddrI; Vdr |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR207155 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGCCGATTCTCTCAGGTGCATGGCGCTGCGCGGTACGGGCCAGTATGGTCTGGCAGCTTTGGGACAC TTCGCACAGTTTACGTTGCCGACCCTACACTTGTGGAGCAGCTCCTGCGACAAGAAAGTCACTGTCCAGA GCGCTGTAGTTTCTCATCATGGGCAGAGCACCGTCGCCGCCACCAGCGTGCTTGCGGATTGCTAACGGCG GATGGTGAAGAATGGCAGAGGCTCCGAAGTCTTCTGGCCCCGCTCCTCCTCCGGCCACAAGCAGCCGCGG GCTATGCTGGAACTCTGGACAACGTGGTCCGTGACCTTGTGCGACGACTAAGGCGCCAGCGGGGACGTGG CTCTGGGCTACCCGGCCTAGTTCTGGACGTGGCAGGAGAGTTTTACAAATTTGGCCTAGAAAGTATAGGC GCGGTGCTGCTGGGATCGCGCCTGGGCTGCCTAGAGGCTGAAGTCCCTCCTGACACAGAAACCTTCATAC ATGCAGTGGGCTCAGTGTCTGTGTCTACACTCTTGACCATGGCGATGCCCAACTGGTTGCACCACCTTAT ACCTGGACCCTGGGCCCGCCTCTGCCGAGACTGGGATCAGATGTTTGCCTTTGCCCAGAGGCACGTGGAG CTGCGAGAAGGTGAAGCTGCGATGAGGAACCAGGGAAAGCCTGAGGAGGATATGCCGTCTGGGCATCACT TAACCCACTTCCTTTTTCGGGAAAAGGTGTCTGTCCAGTCCATAGTGGGGAATGTGACAGAGCTACTACT GGCTGGAGTGGACACGGTATCCAATACGCTCTCCTGGACACTCTATGAGCTTTCCCGGCACCCCGATGTC CAGACTGCACTCCACTCTGAGATCACAGCTGGGACCCGTGGCTCCTGTGCCCACCCCCATGGCACTGCTC TGTCCCAGCTGCCCCTGTTAAAGGCTGTGATCAAAGAAGTGTTGAGATTGTACCCTGTGGTACCTGGGAA TTCCCGTGTCCCAGACAGAGACATCCGTGTAGGAAACTATGTAATTCCCCAAGATACGCTAGTCTCCCTA TGTCACTATGCCACTTCAAGGGACCCCACACAGTTTCCAGACCCCAACTCTTTTAATCCAGCTCGCTGGC TGGGGGAGGGTCCGACCCCCCACCCATTTGCATCTCTTCCCTTCGGCTTTGGCAAACGGAGCTGCATCGG GAGACGCTTGGCAGAGCTTGAGCTACAAATGGCTTTGTCCCAGATCTTGACCCATTTTGAAGTGCTACCT GAGCCAGGTGCTCTTCCTATCAAGCCCATGACCCGGACTGTCCTGGTCCCTGAGAGGAGCATCAATCTAC AGTTTGTAGATAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR207155 protein sequence
Red=Cloning site Green=Tags(s) MRRFSQVHGAARYGPVWSGSFGTLRTVYVADPTLVEQLLRQESHCPERCSFSSWAEHRRRHQRACGLLTA DGEEWQRLRSLLAPLLLRPQAAAGYAGTLDNVVRDLVRRLRRQRGRGSGLPGLVLDVAGEFYKFGLESIG AVLLGSRLGCLEAEVPPDTETFIHAVGSVSVSTLLTMAMPNWLHHLIPGPWARLCRDWDQMFAFAQRHVE LREGEAAMRNQGKPEEDMPSGHHLTHFLFREKVSVQSIVGNVTELLLAGVDTVSNTLSWTLYELSRHPDV QTALHSEITAGTRGSCAHPHGTALSQLPLLKAVIKEVLRLYPVVPGNSRVPDRDIRVGNYVIPQDTLVSL CHYATSRDPTQFPDPNSFNPARWLGEGPTPHPFASLPFGFGKRSCIGRRLAELELQMALSQILTHFEVLP EPGALPIKPMTRTVLVPERSINLQFVDR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010009 |
| ORF Size | 1347 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010009.1, NP_034139.1 |
| RefSeq Size | 2440 bp |
| RefSeq ORF | 1524 bp |
| Locus ID | 13115 |
| UniProt ID | O35084 |
| MW | 49.9 kDa |
| Gene Summary | Catalyzes the conversion of 25-hydroxyvitamin D3 (25(OH)D3) to 1-alpha,25-dihydroxyvitamin D3 (1alpha,25(OH)(2)D3), and of 24,25-dihydroxyvitamin D3 (24,25(OH)(2)D3) to 1-alpha,24,25-trihydroxyvitamin D3 (1alpha,24,25(OH)(3)D3). Is also active with 25-hydroxy-24-oxo-vitamin D3. Plays an important role in normal bone growth, calcium metabolism, and tissue differentiation.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217115 | Cyp27b1 (untagged) - Mouse cytochrome P450, family 27, subfamily b, polypeptide 1 (Cyp27b1), nuclear gene encoding mitochondrial protein, (10ug) |
CNY 5,488.00 |
|
| MG207155 | Cyp27b1 (tGFP-tagged) - Mouse cytochrome P450, family 27, subfamily b, polypeptide 1 (Cyp27b1) |
CNY 4,940.00 |
|
| MR207155L3 | Lenti ORF clone of Cyp27b1 (Myc-DDK-tagged) - Mouse cytochrome P450, family 27, subfamily b, polypeptide 1 (Cyp27b1), nuclear gene encoding mitochondrial protein |
CNY 6,370.00 |
|
| MR207155L4 | Lenti ORF clone of Cyp27b1 (mGFP-tagged) - Mouse cytochrome P450, family 27, subfamily b, polypeptide 1 (Cyp27b1), nuclear gene encoding mitochondrial protein |
CNY 6,370.00 |
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