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Cmas (NM_009908) Mouse Tagged ORF Clone
CAT＃: MR206899
- TrueORF®
Cmas (Myc-DDK-tagged) - Mouse cytidine monophospho-N-acetylneuraminic acid synthetase (Cmas). Note: ORF is codon optimized
ORF Plasmid: tGFP
"NM_009908" in other vectors (4)
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CNY 2,850.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AW208911; CMPNeu5Ac; D6Bwg0250e |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR206899 representing NM_009908, codon optimized.
Due to the complexity of NM_009908, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGCCCTTGAAAAGGGTGCAGCAACATCCGGCCCTGCTCCAAGGGGACGGCCTTCTCGCGGCCGGC CACCAAAGCTTCAAAGGTCAAGGGGCGCGGGAAGAGGTCTCGAGAAACCACCCCACTTGGCAGCACTTGT GCTGGCCCGGGGGGGAAGCAAGGGGATCCCTCTGAAGAACATAAAGAGGTTGGCTGGGGTGCCTCTGATC GGCTGGGTCCTTCGGGCTGCGCTGGATGCTGGAGTTTTCCAGTCCGTGTGGGTATCTACAGACCATGACG AAATCGAAAACGTAGCCAAACAGTTTGGAGCCCAGGTGCACAGGAGAAGTTCAGAAACAAGTAAGGATTC CAGTACAAGCCTGGACGCCATCGTGGAATTTCTGAATTACCACAATGAAGTGGATATCGTTGGCAACATC CAGGCCACGAGCCCCTGCCTGCACCCAACAGATCTGCAGAAGGTGGCCGAGATGATCAGGGAGGAAGGGT ATGATAGTGTTTTCTCTGTTGTCCGGAGGCATCAGTTCCGGTGGTCCGAGATTCAAAAGGGGGTGCGGGA AGTGACAGAACCACTCAACCTCAACCCTGCCAAGCGCCCTCGCCGACAGGATTGGGATGGAGAACTGTAT GAAAACGGGTCTTTTTATTTCGCCAAACGCCACCTGATAGAAATGGGGTACCTGCAGGGAGGCAAGATGG CATATTACGAGATGAGAGCAGAACACTCCGTGGATATCGACGTGGATATCGACTGGCCCATCGCCGAGCA GAGGGTTCTGCGATTTGGCTACTTCGGTAAGGAAAAGTTGAAGGAGATCAAGCTCCTGGTGTGTAACATC GACGGCTGCTTGACCAACGGACATATCTATGTGTCCGGTGACCAGAAAGAGATCATCTCCTACGATGTGA AAGATGCTATCGGTATTAGTCTGTTGAAAAAATCTGGCATCGAGGTTCGATTGATTTCTGAGAGAGCTTG CAGTAAGCAGACTCTCTCTGCACTGAAACTCGATTGCAAAACGGAAGTTTCCGTGTCTGACAAGCTGGCA ACTGTGGATGAGTGGCGCAAAGAGATGGGCCTGTGTTGGAAGGAAGTGGCTTATCTGGGGAATGAAGTTT CCGATGAAGAGTGCCTGAAGCGCGTGGGACTGTCCGCAGTACCAGCTGATGCATGTAGTGGCGCCCAGAA GGCCGTCGGCTATATCTGCAAGTGTAGCGGTGGAAGAGGCGCCATCCGCGAATTCGCAGAGCATATTTTT CTCCTGATTGAGAAGGTCAATAATTCCTGCCAAAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR206899 representing NM_009908
Red=Cloning site Green=Tags(s) MDALEKGAATSGPAPRGRPSRGRPPKLQRSRGAGRGLEKPPHLAALVLARGGSKGIPLKNIKRLAGVPLI GWVLRAALDAGVFQSVWVSTDHDEIENVAKQFGAQVHRRSSETSKDSSTSLDAIVEFLNYHNEVDIVGNI QATSPCLHPTDLQKVAEMIREEGYDSVFSVVRRHQFRWSEIQKGVREVTEPLNLNPAKRPRRQDWDGELY ENGSFYFAKRHLIEMGYLQGGKMAYYEMRAEHSVDIDVDIDWPIAEQRVLRFGYFGKEKLKEIKLLVCNI DGCLTNGHIYVSGDQKEIISYDVKDAIGISLLKKSGIEVRLISERACSKQTLSALKLDCKTEVSVSDKLA TVDEWRKEMGLCWKEVAYLGNEVSDEECLKRVGLSAVPADACSGAQKAVGYICKCSGGRGAIREFAEHIF LLIEKVNNSCQK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009908 |
| ORF Size | 1296 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009908.1, NM_009908.2, NP_034038.2 |
| RefSeq Size | 1760 bp |
| RefSeq ORF | 1299 bp |
| Locus ID | 12764 |
| UniProt ID | Q99KK2 |
| MW | 48 kDa |
| Gene Summary | Catalyzes the activation of N-acetylneuraminic acid (NeuNAc) to cytidine 5'-monophosphate N-acetylneuraminic acid (CMP-NeuNAc), a substrate required for the addition of sialic acid. Has some activity toward NeuNAc, N-glycolylneuraminic acid (Neu5Gc) or 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC201490 | Cmas (untagged) - Mouse cytidine monophospho-N-acetylneuraminic acid synthetase (Cmas), (10ug) |
CNY 2,000.00 |
|
| MG206899 | Cmas (tGFP-tagged) - Mouse cytidine monophospho-N-acetylneuraminic acid synthetase (Cmas). Note: ORF is codon optimized |
CNY 3,140.00 |
|
| MR206899L3 | Lenti ORF clone of Cmas (Myc-DDK-tagged) - Mouse cytidine monophospho-N-acetylneuraminic acid synthetase (Cmas). Note: ORF is codon optimized |
CNY 4,750.00 |
|
| MR206899L4 | Lenti ORF clone of Cmas (mGFP-tagged) - Mouse cytidine monophospho-N-acetylneuraminic acid synthetase (Cmas). Note: ORF is codon optimized |
CNY 4,750.00 |
