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B4galt6 (NM_019737) Mouse Tagged ORF Clone
CAT＃: MR205973
- TrueORF®
B4galt6 (Myc-DDK-tagged) - Mouse UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 6 (B4galt6)
"NM_019737" in other vectors (3)
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CNY 2,850.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AA536803; AU022389 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR205973 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGCGCTCAAGCGGATGATGCGGGTCTCCAATCGCTCTCTGATTGCCTTCATCTTCTTCTTCTCCC TCTCCACGTCCTGTCTCTACTTCATCTATGTGGCTCCAGGCATCGCCAACACGTACCTCTTTATGGTACA AGCTAGAGGTATCATGCTGAGAGAGAATGTGAAAACAATTGGACACATGATCAGGCTGTATACCAATAAA AACACTACGCTCAACGGAACAGATTATCCTGAAGGCAATAATACAAGTGACTATCTTGTTCAAACAACAA CGTATCTTCCGCAAAACTTCACGTATTTGCCACACCTCCCCTGTCCAGAGAAGCTGCCTTACATGCGGGG ATTCCTCAGTGTCAATGTGAGTGAGATCAGTTTTGATGAAGTCCATCAGCTCTTCTCCAAGGACTCAGAG ATTGGGCCAGGAGGCCACTGGAGGCCCAAAGACTGCAAGCCCAGATGGAAGGTGGCAGTTCTCATCCCCT TCCGTAACCGCCATGAACATCTTCCGATTTTTTTCCTGCACCTGATTCCGATGCTCCAGAAACAGCGGCT GGAATTTGCCTTTTATGTCATCGAACAGACCGGCACACAACCATTCAATCGTGCGATGCTCTTTAATGTG GGTTTCAAAGAGGCCATGAAAGACAGAGCCTGGGACTGTGTGATCTTCCATGATGTGGATCATCTGCCTG AAAATGATCGGAACTACTACGGATGTGGAGAGATGCCGCGGCACTTTGCAGCGAAGCTGGACAAGTACAT GTACATTCTTCCCTATAAGGAGTTTTTTGGTGGGGTGAGTGGCCTGACGGTGGAGCAATTCAGGAAGATC AACGGCTTTCCCAATGCCTTCTGGGGATGGGGAGGCGAGGACGATGACCTTTGGAACAGAGTTCACTACG CCGGCTACAATGTAACCCGGCCAGAAGGGGACTTGGGAAAATACATATCTATTCCTCATCACCATCGAGG GGAGGTACAGTTCCTAGGACGGTATAAATTACTTCGGTATTCCAAGGAGCGGCAGTACATCGATGGACTG AACAATTTATTATACACACCCAAAATACTGGTTGATAGGCTGTATACAAATATATCTGTAAACCTCATGC CAGAATTAGCTCCAATTGAAGACTAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR205973 protein sequence
Red=Cloning site Green=Tags(s) MSALKRMMRVSNRSLIAFIFFFSLSTSCLYFIYVAPGIANTYLFMVQARGIMLRENVKTIGHMIRLYTNK NTTLNGTDYPEGNNTSDYLVQTTTYLPQNFTYLPHLPCPEKLPYMRGFLSVNVSEISFDEVHQLFSKDSE IGPGGHWRPKDCKPRWKVAVLIPFRNRHEHLPIFFLHLIPMLQKQRLEFAFYVIEQTGTQPFNRAMLFNV GFKEAMKDRAWDCVIFHDVDHLPENDRNYYGCGEMPRHFAAKLDKYMYILPYKEFFGGVSGLTVEQFRKI NGFPNAFWGWGGEDDDLWNRVHYAGYNVTRPEGDLGKYISIPHHHRGEVQFLGRYKLLRYSKERQYIDGL NNLLYTPKILVDRLYTNISVNLMPELAPIEDY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_019737 |
| ORF Size | 1149 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_019737.2, NP_062711.1 |
| RefSeq Size | 5808 bp |
| RefSeq ORF | 1149 bp |
| Locus ID | 56386 |
| UniProt ID | Q9WVK5 |
| MW | 44.8 kDa |
| Gene Summary | Catalyzes the synthesis of lactosylceramide (LacCer) via the transfer of galactose from UDP-galactose to glucosylceramide (GlcCer) (PubMed:23882130, PubMed:30114188). LacCer is the starting point in the biosynthesis of all gangliosides (membrane-bound glycosphingolipids) which play pivotal roles in the CNS including neuronal maturation and axonal and myelin formation (PubMed:30114188).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC210047 | B4galt6 (untagged) - Mouse UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 6 (B4galt6), (10ug) |
CNY 3,990.00 |
|
| MR205973L3 | Lenti ORF clone of B4galt6 (Myc-DDK-tagged) - Mouse UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 6 (B4galt6) |
CNY 4,750.00 |
|
| MR205973L4 | Lenti ORF clone of B4galt6 (mGFP-tagged) - Mouse UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 6 (B4galt6) |
CNY 4,750.00 |
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