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Tbp (NM_013684) Mouse Tagged ORF Clone
CAT＃: MR204589
- TrueORF®
Tbp (Myc-DDK-tagged) - Mouse TATA box binding protein (Tbp)
ORF Plasmid: tGFP
"NM_013684" in other vectors (4)
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CNY 2,081.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Gtf2d; GTF2D1; SCA17; TFIID |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR204589 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACCAGAACAACAGCCTTCCACCTTATGCTCAGGGCTTGGCCTCCCCACAGGGCGCCATGACTCCTG GAATTCCCATCTTTAGTCCAATGATGCCTTACGGCACAGGACTTACTCCACAGCCTATTCAGAACACCAA CAGTCTCTCTATTTTGGAAGAGCAACAAAGACAGCAGCAGCAACAGCAACAGCAGCAGCAACAACAGCAG GCAGTAGCAACTGCAGCAGCCTCAGTACAGCAATCAACATCTCAGCAACCCACACAGGGTGCCTCAGGCC AGACCCCACAACTCTTCCATTCTCAAACTCTGACCACTGCACCGTTGCCAGGCACCACCCCCTTGTACCC TTCACCAATGACTCCTATGACCCCTATCACTCCTGCCACACCAGCTTCTGAGAGCTCTGGAATTGTACCG CAGCTTCAAAATATTGTATCTACCGTGAATCTTGGCTGTAAACTTGACCTAAAGACCATTGCACTTCGTG CAAGAAATGCTGAATATAATCCCAAGCGATTTGCTGCAGTCATCATGAGAATAAGAGAGCCACGGACAAC TGCGTTGATTTTCAGTTCTGGAAAAATGGTGTGCACAGGAGCCAAGAGCTATGAGCCAGAATTATTTCCT GGATTAATCTACAGAATGATCAAACCCAGAATTGTTCTCCTTATTTTTGTTTCTGGAAAAGTTGTATTAA CAGGTGCTAAAGTTAGAGCAGAGATTTATGAAGCATTTGAAAACATCTACCCCATCTTAAAGGGATTCAG GAAGACCACA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR204589 protein sequence
Red=Cloning site Green=Tags(s) MDQNNSLPPYAQGLASPQGAMTPGIPIFSPMMPYGTGLTPQPIQNTNSLSILEEQQRQQQQQQQQQQQQQ AVATAAASVQQSTSQQPTQGASGQTPQLFHSQTLTTAPLPGTTPLYPSPMTPMTPITPATPASESSGIVP QLQNIVSTVNLGCKLDLKTIALRARNAEYNPKRFAAVIMRIREPRTTALIFSSGKMVCTGAKSYEPELFP GLIYRMIKPRIVLLIFVSGKVVLTGAKVRAEIYEAFENIYPILKGFRKTT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_013684 |
| ORF Size | 783 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_013684.2, NP_038712.2 |
| RefSeq Size | 1842 bp |
| RefSeq ORF | 951 bp |
| Locus ID | 21374 |
| UniProt ID | P29037 |
| MW | 28.3 kDa |
| Gene Summary | General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of a BRF2-containing transcription factor complex that regulates transcription mediated by RNA polymerase III. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (pre-initiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC207415 | Tbp (untagged) - Mouse TATA box binding protein (Tbp), (10ug) |
CNY 3,990.00 |
|
| MG204589 | Tbp (tGFP-tagged) - Mouse TATA box binding protein (Tbp) |
CNY 2,850.00 |
|
| MR204589L3 | Lenti ORF clone of Tbp (Myc-DDK-tagged) - Mouse TATA box binding protein (Tbp) |
CNY 4,750.00 |
|
| MR204589L4 | Lenti ORF clone of Tbp (mGFP-tagged) - Mouse TATA box binding protein (Tbp) |
CNY 4,750.00 |
