Mff (NM_029409) Mouse Tagged ORF Clone
CAT#: MR203974
- TrueORF®
Mff (Myc-DDK-tagged) - Mouse mitochondrial fission factor (Mff), nuclear gene encoding mitochondrial protein
ORF Plasmid: tGFP
"NM_029409" in other vectors (6)
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CNY 3,600.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 5230400G24Rik; AI314724 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR203974 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGAAATTAGTCGAATTCAGTATGAAATGGAATACACCGAAGGTATTAGTCAGCGAATGAGGGTCC CGGAAAAATTAAAAGTAGCACCACCAAATGCTGACCTGGAGCAAGAGTTCCAAGATGGAGTTCCAAATGC CAGTGTGATAATGCAAGTCCCAGAGAGGATCGTGGTTACAGGAAATAATGAAGACATTTCATTTTCAAGA CCAGCAGATCTTGACCTTATCCAGTCCACTCCCTTTAAACCTCTGGCACTGAAAACACCACCACGCGTGC TCACACTGAGTGAGAGACCACTAGATTTCCTGGATTTAGAAAGACCACTTCCAACTCCTCAAAGCGAAGA GAGCCGAGCAGTTGGCAGGCTAAAAAGAGAGCGTTCTATGAGTGAAAATGCTGTTCGCCAAAATGGACAG TTGGTCAGAAACGATTCCATTGTGACACCATCACCACCACAGGCTCGGGTCTGTCCTCCCCATATGCTAC CTGAAGATGGAGCTAATCTTTCCTCTGCCCGTGGCATTTTGTCGCTTATCCAGTCTTCTACTCGTAGGGC TTACCAGCAGATCTTGGATGTGCTGGATGAAAACCGCAGACCTGTGTTGCGTGGTGGGTCAGCTGCCGCC ACTTCTAATCCTCATCATGACAACGTCAGGTATGGCATTTCAAACCTAGATGCAGCCATTGAAGGAGCTT CAGATGACATGACTGTGGTAGATGCAGCTTCATTAAGACGTCAGATAATCAAATTAAATAGACGTCTCCA ACTTCTGGAAGAGGAGAATAAAGAGCGTGCTAAAAGAGAAATGGTCATGTATTCAATCACTGTAGCGTTC TGGCTGCTTAACAGCTGGCTCTGGTTTCGACGC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >MR203974 protein sequence
Red=Cloning site Green=Tags(s) MAEISRIQYEMEYTEGISQRMRVPEKLKVAPPNADLEQEFQDGVPNASVIMQVPERIVVTGNNEDISFSR PADLDLIQSTPFKPLALKTPPRVLTLSERPLDFLDLERPLPTPQSEESRAVGRLKRERSMSENAVRQNGQ LVRNDSIVTPSPPQARVCPPHMLPEDGANLSSARGILSLIQSSTRRAYQQILDVLDENRRPVLRGGSAAA TSNPHHDNVRYGISNLDAAIEGASDDMTVVDAASLRRQIIKLNRRLQLLEEENKERAKREMVMYSITVAF WLLNSWLWFRR SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-RsrII Cloning Scheme for this gene Plasmid Map |
ACCN | NM_029409 |
ORF Size | 876 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_029409.3, NP_083685.2 |
RefSeq Size | 1920 bp |
RefSeq ORF | 876 bp |
Locus ID | 75734 |
UniProt ID | Q6PCP5 |
MW | 32.9 kDa |
Gene Summary | Plays a role in mitochondrial and peroxisomal fission. Promotes the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface. May be involved in regulation of synaptic vesicle membrane dynamics by recruitment of DNM1L to clathrin-containing vesicles.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC207757 | Mff (untagged) - Mouse mitochondrial fission factor (Mff), nuclear gene encoding mitochondrial protein, (10ug) |
CNY 3,990.00 |
|
MG203974 | Mff (tGFP-tagged) - Mouse RIKEN cDNA 5230400G24 gene (5230400G24Rik) |
CNY 4,370.00 |
|
MR203974L1 | Lenti ORF clone of Mff (Myc-DDK-tagged) - Mouse mitochondrial fission factor (Mff), nuclear gene encoding mitochondrial protein |
CNY 5,890.00 |
|
MR203974L2 | Lenti ORF clone of Mff (mGFP-tagged) - Mouse mitochondrial fission factor (Mff), nuclear gene encoding mitochondrial protein |
CNY 6,000.00 |
|
MR203974L3 | Lenti ORF clone of Mff (Myc-DDK-tagged) - Mouse mitochondrial fission factor (Mff), nuclear gene encoding mitochondrial protein |
CNY 6,000.00 |
|
MR203974L4 | Lenti ORF clone of Mff (mGFP-tagged) - Mouse mitochondrial fission factor (Mff), nuclear gene encoding mitochondrial protein |
CNY 5,890.00 |