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Gpm6a (NM_153581) Mouse Tagged ORF Clone
CAT＃: MR203739
- TrueORF®
Gpm6a (Myc-DDK-tagged) - Mouse glycoprotein m6a (Gpm6a)
ORF Plasmid: tGFP
"NM_153581" in other vectors (4)
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CNY 2,081.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Gpm6; M6A |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR203739 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGAGAATATGGAAGAAGGACAGACACAGAAAGGGTGCTTCGAGTGCTGCATTAAATGCCTGGGAG GTATTCCCTATGCTTCTCTGATTGCAACCATCCTGCTGTATGCAGGCGTTGCCCTGTTCTGTGGCTGTGG CCATGAAGCCCTTTCTGGAACAGTCAACATTCTGCAGACCTACTTTGAGTTGGCAAGGACTGCTGGAGAC ACACTGGATGTTTTCACTATGATTGACATCTTTAAGTATGTGATCTATGGCATTGCGGCTGCTTTCTTTG TCTATGGCATTTTACTGATGGTAGAAGGTTTCTTCACAACTGGGGCTATCAAAGATCTCTATGGAGACTT CAAAATCACCACCTGTGGCAGATGTGTGAGCGCTTGGTTTATCATGCTGACATACCTCTTCATGTTGGCC TGGCTGGGAGTCACAGCTTTCACCTCACTGCCCGTGTACATGTATTTCAATGTGTGGACCATCTGCCGGA ACACCACTCTAGTGGAGGGAGCAAATCTCTGCTTGGATCTGCGTCAGTTTGGGATTGTGACAATTGGAGA GGAAAAGAAAATTTGTACTGCCTCTGAGAACTTCCTGAGGATGTGTGAATCTACTGAGCTGAATATGACC TTCCACTTGTTCATTGTGGCACTTGCTGGAGCTGGAGCAGCAGTTATTGCTATGGTCCACTACCTGATGG TTCTGTCTGCCAACTGGGCCTATGTGAAAGATGCCTGCCGCATGCAGAAGTACGAAGACATCAAGTCAAA GGAAGAGCAGGAGCTGCACGACATCCATTCTACTCGCTCCAAAGAGCGGCTCAATGCGTACACA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR203739 protein sequence
Red=Cloning site Green=Tags(s) MEENMEEGQTQKGCFECCIKCLGGIPYASLIATILLYAGVALFCGCGHEALSGTVNILQTYFELARTAGD TLDVFTMIDIFKYVIYGIAAAFFVYGILLMVEGFFTTGAIKDLYGDFKITTCGRCVSAWFIMLTYLFMLA WLGVTAFTSLPVYMYFNVWTICRNTTLVEGANLCLDLRQFGIVTIGEEKKICTASENFLRMCESTELNMT FHLFIVALAGAGAAVIAMVHYLMVLSANWAYVKDACRMQKYEDIKSKEEQELHDIHSTRSKERLNAYT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_153581 |
| ORF Size | 837 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_153581.6 |
| RefSeq Size | 3351 bp |
| RefSeq ORF | 837 bp |
| Locus ID | 234267 |
| UniProt ID | P35802 |
| MW | 31.1 kDa |
| Gene Summary | Involved in neuronal differentiation, including differentiation and migration of neuronal stem cells. Plays a role in neuronal plasticity and is involved in neurite and filopodia outgrowth, filopodia motility and probably synapse formation. Gpm6a-induced filopodia formation involves mitogen-activated protein kinase (MAPK) and Src signaling pathways. Conflictingly, PubMed:22162747 reports that induced cellular protrusions are simple membrane-wrapped tubules without actin or tubulin-based cytoskeletons and with Gpm6a gliding along membrane edges indicative for a function in actin-independent membrane deformation. May be involved in neuronal NGF-dependent Ca(2+) influx. May be involved in regulation of endocytosis and intracellular trafficking of G-protein-coupled receptors (GPCRs); enhances internalization and recycling of mu-type opioid receptor.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC202182 | Gpm6a (untagged) - Mouse glycoprotein m6a (Gpm6a), (10ug) |
CNY 2,000.00 |
|
| MG203739 | Gpm6a (tGFP-tagged) - Mouse glycoprotein m6a (Gpm6a) |
CNY 2,850.00 |
|
| MR203739L3 | Lenti ORF clone of Gpm6a (Myc-DDK-tagged) - Mouse glycoprotein m6a (Gpm6a) |
CNY 4,750.00 |
|
| MR203739L4 | Lenti ORF clone of Gpm6a (mGFP-tagged) - Mouse glycoprotein m6a (Gpm6a) |
CNY 4,750.00 |
