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Cish (NM_009895) Mouse Tagged ORF Clone
CAT＃: MR203328
- TrueORF®
Cish (Myc-DDK-tagged) - Mouse cytokine inducible SH2-containing protein (Cish)
ORF Plasmid: tGFP
"NM_009895" in other vectors (3)
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CNY 1,416.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Cis; CIS-1; CIS1; F17; F23; SOCS |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR203328 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCCTCTGCGTACAGGGATCTTGTCCTTTGCTGGCTGTGGAGCAAATTGGGCGGCGGCCTCTGTGGG CCCAGTCCCTGGAGCTGCCCGGGCCAGCCATGCAGCCCTTACCCACTGGGGCATTCCCAGAGGAAGTGAC AGAGGAGACCCCTGTCCAGGCAGAGAATGAACCGAAGGTGCTAGACCCTGAGGGGGATCTGCTGTGCATA GCCAAGACGTTCTCCTACCTTCGGGAATCTGGGTGGTACTGGGGTTCTATTACAGCCAGCGAGGCCCGGC AGCACCTACAGAAGATGCCGGAGGGTACATTCCTAGTTCGAGACAGCACCCACCCCAGCTACCTGTTCAC ACTGTCAGTCAAAACCACCCGTGGCCCCACCAACGTGCGGATCGAGTACGCCGATTCTAGCTTCCGGCTG GACTCTAACTGCTTGTCAAGACCTCGAATCCTGGCCTTCCCAGATGTGGTCAGCCTTGTGCAGCACTATG TGGCCTCCTGTGCAGCTGACACCCGGAGCGACAGCCCGGATCCTGCTCCCACCCCAGCCCTGCCTATGTC TAAGCAAGATGCACCTAGTGACTCGGTGCTGCCTATCCCCGTGGCTACTGCAGTGCACCTGAAACTGGTG CAGCCCTTTGTGCGCAGGAGCAGTGCCCGCAGCTTACAACATCTGTGTCGGCTAGTCATCAACCGTCTGG TGGCCGACGTGGACTGCTTACCCCTGCCCCGGCGTATGGCCGACTACCTCCGACAGTACCCCTTCCAACT C ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR203328 protein sequence
Red=Cloning site Green=Tags(s) MVLCVQGSCPLLAVEQIGRRPLWAQSLELPGPAMQPLPTGAFPEEVTEETPVQAENEPKVLDPEGDLLCI AKTFSYLRESGWYWGSITASEARQHLQKMPEGTFLVRDSTHPSYLFTLSVKTTRGPTNVRIEYADSSFRL DSNCLSRPRILAFPDVVSLVQHYVASCAADTRSDSPDPAPTPALPMSKQDAPSDSVLPIPVATAVHLKLV QPFVRRSSARSLQHLCRLVINRLVADVDCLPLPRRMADYLRQYPFQL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009895 |
| ORF Size | 774 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009895.1 |
| RefSeq Size | 2161 bp |
| RefSeq ORF | 774 bp |
| Locus ID | 12700 |
| UniProt ID | Q62225 |
| MW | 28.5 kDa |
| Gene Summary | SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. CIS is involved in the negative regulation of cytokines that signal through the JAK-STAT5 pathway such as erythropoietin, prolactin and interleukin 3 (IL3) receptor. Inhibits STAT5 trans-activation by suppressing its tyrosine phosphorylation. May be a substrate-recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Interaction of suppressor of cytokine signalling 3 with cavin-1 links SOCS3 function and cavin-1 stability
,Williams, JJL;Alotaiq, N;Mullen, W;Burchmore, R;Liu, L;Baillie, GS;Schaper, F;Pilch, PF;Palmer, TM;,
Nat Commun
,PubMed ID 29330478
[Cish]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG203328 | Cish (tGFP-tagged) - Mouse cytokine inducible SH2-containing protein (Cish) |
CNY 2,850.00 |
|
| MR203328L3 | Lenti ORF clone of Cish (Myc-DDK-tagged) - Mouse cytokine inducible SH2-containing protein (Cish) |
CNY 4,750.00 |
|
| MR203328L4 | Lenti ORF clone of Cish (mGFP-tagged) - Mouse cytokine inducible SH2-containing protein (Cish) |
CNY 4,750.00 |
