Fam192a (NM_028221) Mouse Tagged ORF Clone

CAT#: MR203251

  • TrueORF®

Fam192a (Myc-DDK-tagged) - Mouse family with sequence similarity 192, member A (Fam192a)

ORF Plasmid: DDK tGFP



  "NM_028221" in other vectors (5)

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Mouse Protein / Lysate Request

CNY 1,416.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms 1700001O11Rik; 2310065K24Rik; AI596259; Nip30
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR203251 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATGGAGAGGACGATAGTAACCTTGTCATCAAAAAGAGGTTTGTGTCTGAGGCAGAGCTAGATGAGC
GGCGCAAAAGGAGGCAAGAAGAATGGGAGAAAGTGCGAAAACCAGAAGACCCAAAAGAATGTCCAGAGGA
GGCGTATGACCCTCGGTCTCTGTACGAACGGCTGCAGGAACAGAAAGACAGGAAGCAGCAGGAGTATGAG
GAGCAATTCAAATTCAAAAACATGGTAAGAGGGTTAGACGAAGATGAGACCAACTTCCTTGATGAGGTTT
CTCGGCAGCAGGAGCTGATAGAGAAGCAGCGGCGAGAAGAAGAACTAGAGGAACTGAAGGAGTACAGAAG
TAATCTCAACAAGGTTGGAATTTCTGCAGAAAATAAAGAAGTAGAGAAGAAGCTGGCCGTGAAACCCATA
GAAACCAAGAACAAGTTCTCCCAGGCAAAGCTGTTGGCAGGAGCTGTGAAACATAAGAGCTCAGAGAGTG
GCAATAGTGTGAAGAGACTGAAACCAGACCCCGACCCAGATGACAAGGCTCAAGAGGCCCCGTCCTGCAT
GTCTCTTGGAAGCTCTTCTCTGAGCGGACCTCCCTCCATCCACTGCCCGTCGGCTGCTGTGTGCATCGGC
ATCCTCCCTGGCTTGGGCGCCTACTCTGGGAGCAGTGACTCCGAATCCAGTTCAGACAGTGAAGGCACCA
TCAATGCTACAGGGAAGATCGTCTCCTCCATCTTCCGAACCAACACCTTCCTCGAGGCACCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR203251 protein sequence
Red=Cloning site Green=Tags(s)

MDGEDDSNLVIKKRFVSEAELDERRKRRQEEWEKVRKPEDPKECPEEAYDPRSLYERLQEQKDRKQQEYE
EQFKFKNMVRGLDEDETNFLDEVSRQQELIEKQRREEELEELKEYRSNLNKVGISAENKEVEKKLAVKPI
ETKNKFSQAKLLAGAVKHKSSESGNSVKRLKPDPDPDDKAQEAPSCMSLGSSSLSGPPSIHCPSAAVCIG
ILPGLGAYSGSSDSESSSDSEGTINATGKIVSSIFRTNTFLEAP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_028221
ORF Size 765 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_028221.4, NP_082497.2
RefSeq Size 1812 bp
RefSeq ORF 765 bp
Locus ID 102122
UniProt ID Q91WE2
MW 28.7 kDa
Gene Summary Promotes the association of the proteasome activator complex subunit PSME3 with the 20S proteasome and regulates its activity. Inhibits PSME3-mediated degradation of some proteasome substrates, probably by affecting their diffusion rate into the catalytic chamber of the proteasome. Also inhibits the interaction of PSME3 with COIL, inhibits accumulation of PSME3 in Cajal bodies and positively regulates the number of Cajal bodies in the nucleus.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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