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Cldn10 (NM_021386) Mouse Tagged ORF Clone
CAT＃: MR202789
- TrueORF®
Cldn10 (Myc-DDK-tagged) - Mouse claudin 10 (Cldn10), transcript variant b
ORF Plasmid: tGFP
"NM_021386" in other vectors (4)
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CNY 2,081.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 6720456I16Rik; Cldn; Cldn1; Cldn10a; Cldn10b; D14Ertd728; D14Ertd728e |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR202789 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTAGCACGGCCTTGGAAATCGTCGCCTTCGTAGTCTCCATCTCGGGCTGGGTGCTAGTGTCTTCCA CACTGCCCACCGACTACTGGAAGGTCTCCACCATCGATGGCACTGTCATCACCACAGCCACTTATTTTGC CAACCTGTGGAAGATCTGCGTTACCGATTCCACCGGTGTCGCCAACTGCAAGGAGTTCCCCTCCATGCTG GCGTTGGATGGTTACATCCAGGCATGTAGAGGACTAATGATCGCTGCGGTCAGCCTGGGATTTTTCGGTT CCATTTTTGCACTCTTTGGAATGAAATGTACCAAAGTCGGAGGCTCAGATCAAGCCAAAGCTAAAATCGC TTGCTTGGCCGGGATTGTATTCATATTGTCAGGTCTGTGTTCCATGACAGGCTGTTCCCTGTATGCAAAC AAAATCACAACAGAATTCTTTGATCCTCTCTATATGGAGCAAAAGTATGAATTAGGGGCTGCTCTCTTCA TCGGATGGGCAGGAGCTTCTCTCTGCATCATTGGGGGAGTCATATTTTGCTTTTCAATATCCGACAACAA TAAGACACCCAGAATGGGCTACACATACAACGGACCCACGTCTGTCATGTCTTCTCGGACCAAGTATCAA GGCGGAGAAGGAGATTTTAAAACCGCAGGCCCTTCAAAACAGTTTGATAAAAATGCCTATGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR202789 protein sequence
Red=Cloning site Green=Tags(s) MASTALEIVAFVVSISGWVLVSSTLPTDYWKVSTIDGTVITTATYFANLWKICVTDSTGVANCKEFPSML ALDGYIQACRGLMIAAVSLGFFGSIFALFGMKCTKVGGSDQAKAKIACLAGIVFILSGLCSMTGCSLYAN KITTEFFDPLYMEQKYELGAALFIGWAGASLCIIGGVIFCFSISDNNKTPRMGYTYNGPTSVMSSRTKYQ GGEGDFKTAGPSKQFDKNAYV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_021386 |
| ORF Size | 696 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_021386.3 |
| RefSeq Size | 960 bp |
| RefSeq ORF | 696 bp |
| Locus ID | 58187 |
| UniProt ID | Q9Z0S6 |
| MW | 24.7 kDa |
| Gene Summary | This intronless gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight unction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. Six alternatively spliced transcript variants have been identified, which encode different isoforms with distinct electric charge of the first extracellular loop and with or without the fourth transmembrane region. These isoforms exhibit distinct localization and function in paracellular anion or cation permeability. [provided by RefSeq, Aug 2010] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Expression Patterns of Claudin Family Members During Tooth Development and the Role of Claudin-10 (Cldn10) in Cytodifferentiation of Stratum Intermedium
,Wang, X;Chiba, Y;Jia, L;Yoshizaki, K;Saito, K;Yamada, A;Qin, M;Fukumoto, S;,
Front. Cell Dev. Biol.
,PubMed ID 33195274
[CLDN10]
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Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC204128 | Cldn10 (untagged) - Mouse claudin 10 (Cldn10), transcript variant b, (10ug) |
CNY 2,000.00 |
|
| MG202789 | Cldn10 (tGFP-tagged) - Mouse claudin 10 (Cldn10), transcript variant 2 |
CNY 2,850.00 |
|
| MR202789L3 | Lenti ORF clone of Cldn10 (Myc-DDK-tagged) - Mouse claudin 10 (Cldn10), transcript variant b |
CNY 4,750.00 |
|
| MR202789L4 | Lenti ORF clone of Cldn10 (mGFP-tagged) - Mouse claudin 10 (Cldn10), transcript variant b |
CNY 4,750.00 |
