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Ak1 (BC014802) Mouse Tagged ORF Clone
CAT＃: MR201878
- TrueORF®
Ak1 (Myc-DDK-tagged) - Mouse adenylate kinase 1 (cDNA clone MGC:25242 IMAGE:4505142)
ORF Plasmid: tGFP
"BC014802" in other vectors (3)
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CNY 2,400.00
CNY 2,945.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Ak-1; B430205N08Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR201878 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGAGAAGCTGAAGAAGGCCAAGATCATCTTTGTGGTGGGCGGACCTGGCTCAGGAAAGGGCACCC AGTGCGAGAAGATTGTACAGAAATATGGCTACACCCACCTGTCTACTGGGGACCTGCTTCGGGCAGAAGT CAGCTCTGGATCGGAGAGGGGCAAGAAGCTGTCGGCTATCATGGAGAAGGGAGAGCTAGTGCCACTGGAC ACGGTGCTGGACATGCTCCGAGATGCTATGTTAGCCAAAGTGGATTCTTCCAATGGCTTCCTGATCGACG GCTACCCGAGGGAGGTGAAACAGGGAGAAGAATTTGAACAGAAGATTGGACAGCCCACACTGCTGCTGTA TGTGGACGCAGGCGCCGAGACCATGACCCAACGACTCCTGAAGCGAGGGGAGACCAGTGGCCGCGTGGAT GACAACGAGGAGACCATCAAGAAGCGGCTGGAGACTTATTACAATGCCACAGAACCTGTCATCTCCTTCT ATGACAAGCGTGGCATTGTGCGCAAGGTCAATGCCGAAGGCACGGTGGACACTGTCTTCTCTGAGGTCTG CACCTATCTTGACTCCCTGAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR201878 protein sequence
Red=Cloning site Green=Tags(s) MEEKLKKAKIIFVVGGPGSGKGTQCEKIVQKYGYTHLSTGDLLRAEVSSGSERGKKLSAIMEKGELVPLD TVLDMLRDAMLAKVDSSNGFLIDGYPREVKQGEEFEQKIGQPTLLLYVDAGAETMTQRLLKRGETSGRVD DNEETIKKRLETYYNATEPVISFYDKRGIVRKVNAEGTVDTVFSEVCTYLDSLK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC014802 |
| ORF Size | 582 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | BC014802, AAH14802 |
| RefSeq Size | 2059 bp |
| RefSeq ORF | 584 bp |
| Locus ID | 11636 |
| MW | 21.5 kDa |
| Gene Summary | Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Also possesses broad nucleoside diphosphate kinase activity. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism (By similarity). May provide a mechanism to buffer the adenylate energy charge for sperm motility.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG201878 | Ak1 (tGFP-tagged) - Mouse adenylate kinase 1 (cDNA clone MGC:25242 IMAGE:4505142) |
CNY 2,850.00 |
|
| MR201878L3 | Lenti ORF clone of Ak1 (Myc-DDK-tagged) - Mouse adenylate kinase 1 (cDNA clone MGC:25242 IMAGE:4505142) |
CNY 4,750.00 |
|
| MR201878L4 | Lenti ORF clone of Ak1 (mGFP-tagged) - Mouse adenylate kinase 1 (cDNA clone MGC:25242 IMAGE:4505142) |
CNY 4,750.00 |
