United States
Germany
Japan
United Kingdom
China
Smad9 (NM_019483) Mouse Tagged ORF Clone
CAT＃: MG226782
- TrueORF®
Smad9 (tGFP-tagged) - Mouse MAD homolog 9 (Drosophila) (Smad9), (10ug)
ORF Plasmid: DDK
"NM_019483" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 4,750.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Madh; MADH6; Madh8; Madh9; SMA; SMAD; Smad8 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG226782 representing NM_019483
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCACCCCAGCACCCCCATCAGCTCCCTCTTCTCCTTCACCAGCCCCGCAGTGAAGCGGCTGCTGGGCT GGAAGCAGGGAGATGAAGAGGAGAAGTGGGCAGAGAAGGCGGTGGACTCTTTGGTGAAGAAGTTAAAGAA GAAGAAAGGCGCCATGGATGAACTGGAGAGGGCGCTGAGCTGCCCGGGTCAGCCTAGCAAGTGTGTCACC ATCCCACGGTCCCTCGATGGACGCCTCCAGGTGTCCCACCGAAAGGGGCTGCCCCACGTCATCTACTGCC GCGTGTGGCGCTGGCCAGACCTGCAGTCCCATCATGAGCTGAAGCCCTTGGAGTGCTGTGAGTTCCCGTT CGGCTCCAAGCAGAAGGAGGTCTGCATCAACCCATACCATTACCGCAGAGTGGAGACCCCAGTTCTGCCT CCAGTGCTGGTACCAAGACACAGCGAGTACAACCCTCAGCTCAGCCTCCTGGCCAAGTTCCGAAGTGCCT CGCTGCACAGCGAACCCCTCATGCCGCACAACGCCACCTACCCTGACTCTTTCCAGCAGTCTCTCTGTCC GGCACCGCCCTCCTCGCCAGGCCATGTGTTTCCGCAGTCTCCATGCCCCACCAGCTACCCGCACTCCCCC GGAAGTCCTTCAGACAGTCCCTATCAACACTCAGACTTCCGGCCAGTTTGCTACGAGGAACCCCAGCACT GGTGTTCTGTTGCCTACTACGAACTAAACAACCGGGTCGGAGAGACTTTCCAGGCGTCCTCGCGGAGCGT GCTCATAGACGGCTTCACCGACCCTTCCAATAACAGGAATAGGTTTTGCCTTGGGCTTCTCTCAAATGTA AACAGAAACTCGACCATAGAAAACACCAGGAGGCACATTGGAAAGGGTGTGCATTTGTACTACGTTGGGG GCGAGGTGTATGCGGAGTGCGTGAGCGACAGCAGCATCTTTGTCCAGAGCCGGAACTGCAACTACCAGCA CGGCTTCCACCCGGCCACCGTCTGCAAGATCCCCAGCGGCTGCAGCCTCAAGGTCTTCAACAACCAGCTC TTCGCCCAGCTGCTCGCCCAGTCCGTGCACCACGGCTTTGAAGTGGTGTATGAGCTGACGAAGATGTGCA CGATTCGGATGAGCTTTGTGAAGGGCTGGGGAGCAGAGTATCATCGCCAGGATGTCACGAGCACCCCCTG CTGGATCGAGATCCATCTTCATGGACCGCTGCAGTGGTTGGATAAGGTGCTCACTCAGATGGGCTCCCCA CACAACCCTATCTCTTCAGTGTCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226782 representing NM_019483
Red=Cloning site Green=Tags(s) MHPSTPISSLFSFTSPAVKRLLGWKQGDEEEKWAEKAVDSLVKKLKKKKGAMDELERALSCPGQPSKCVT IPRSLDGRLQVSHRKGLPHVIYCRVWRWPDLQSHHELKPLECCEFPFGSKQKEVCINPYHYRRVETPVLP PVLVPRHSEYNPQLSLLAKFRSASLHSEPLMPHNATYPDSFQQSLCPAPPSSPGHVFPQSPCPTSYPHSP GSPSDSPYQHSDFRPVCYEEPQHWCSVAYYELNNRVGETFQASSRSVLIDGFTDPSNNRNRFCLGLLSNV NRNSTIENTRRHIGKGVHLYYVGGEVYAECVSDSSIFVQSRNCNYQHGFHPATVCKIPSGCSLKVFNNQL FAQLLAQSVHHGFEVVYELTKMCTIRMSFVKGWGAEYHRQDVTSTPCWIEIHLHGPLQWLDKVLTQMGSP HNPISSVS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_019483 |
| ORF Size | 1290 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_019483.2 |
| RefSeq Size | 5368 bp |
| RefSeq ORF | 1293 bp |
| Locus ID | 55994 |
| UniProt ID | Q9JIW5 |
| Gene Summary | This gene encodes a member of a family of proteins that act as downstream effectors of the bone morphogenetic protein (BMP) signaling pathway. The encoded protein is phosphorylated by BMP receptors, which stimulates its binding to SMAD4 and translocation into the nucleus, where it functions as a regulator of transcription. Activity of this protein is important for embryonic development. Mutation of this gene results in defects in pulmonary vasculature. [provided by RefSeq, Mar 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC210007 | Smad9 (untagged) - Mouse MAD homolog 9 (Drosophila) (Smad9), (10ug) |
CNY 5,488.00 |
|
| MR226782 | Smad9 (Myc-DDK-tagged) - Mouse MAD homolog 9 (Drosophila) (Smad9) |
CNY 5,488.00 |
|
| MR226782L3 | Lenti ORF clone of Smad9 (Myc-DDK-tagged) - Mouse MAD homolog 9 (Drosophila) (Smad9) |
CNY 6,180.00 |
|
| MR226782L4 | Lenti ORF clone of Smad9 (mGFP-tagged) - Mouse MAD homolog 9 (Drosophila) (Smad9) |
CNY 6,180.00 |
