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Kcnc3 (NM_008422) Mouse Tagged ORF Clone
CAT＃: MG226697
- TrueORF®
Kcnc3 (tGFP-tagged) - Mouse potassium voltage gated channel Shaw-related subfamily member 3 (Kcnc3), (10ug)
ORF Plasmid: DDK
"NM_008422" in other vectors (4)
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CNY 6,370.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Kcr2-3; KShIIID; Kv3.3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG226697 representing NM_008422
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCAGTTCAGTGTGCGTCTGGTCGTTCCGCGGGCGCCAGGGGACCGGCAAGCAGCAGCCTCAGCCGG TGCCAACGCCGCAGCCGCCTGAGTCCTCACCGCCGCCTCTGCCGCCGCCGCAGCAGCAGCAGTGCTCTCA GCCCGGCACTGCCGCCTCCCCGGCGGGTGCCCCGCTTTCCTGCGGGCCTGGGGGCCGGCGTGCCGAGCCA TGCCCCGGGCTGCCGGCGGTGGCCATGGGGCGGCACGGCGGCGGCGGCGGCGACAGCGGTAAGATCGTGA TCAACGTGGGCGGCGTGCGCCATGAGACGTACCGCTCCACGTTGCGCACCCTGCCAGGGACCAGACTGGC CGGGCTGACCGAGCCCGAGGCGGCCGCGCGCTTTGACTACGACCCGGGCACGGACGAGTTCTTCTTCGAC CGTCACCCGGGCGTCTTCGCCTACGTGCTCAACTACTACCGCACCGGCAAACTGCACTGCCCGGCCGACG TGTGCGGGCCGCTCTTCGAGGAGGAGCTGGGCTTCTGGGGCATAGACGAGACGGACGTGGAGGCCTGCTG CTGGATGACCTATCGCCAGCACCGTGACGCTGAGGAGGCACTGGACTCTTTCGAGGCCCCGGACTCCTCG GCCAACGCCAACGCCAACGCCGGAGGCGCGCACGATGCGGGACTGGACGACGAGGCGGGCGCAGGAGGCG GCGGCCTGGACGGGGCAGGCGGCGAGCTCAAGCGTCTGTGTTTTCAGGACGCGGGCGGAGGTGCCGGAGG ACCTGCCGGGGGCGCGGGCGGCGCGGGCGGCACCTGGTGGCGGCGCTGGCAGCCCCGTGTGTGGGCGCTT TTTGAGGACCCCTACTCGTCGCGGGCTGCCAGGTATGTGGCCTTCGCCTCCCTATTCTTTATCCTCATCT CCATCACCACCTTCTGCCTGGAGACACACGAGGGCTTCATCCACATCAGCAACAAGACGGTGACGCAGGC CTCCCCAATCCCTGGGGCTCCCCCGGAGAATATCACCAACGTGGAGGTGGAGACGGAACCCTTCTTGACC TACGTGGAAGGCGTGTGTGTGGTCTGGTTCACCTTTGAGTTTCTCATGCGGGTCACCTTCTGCCCAGATA AGGTGGAGTTTCTCAAAAGCAGCCTGAACATCATCGACTGCGTGGCCATCTTGCCCTTCTACTTGGAAGT GGGCCTGTCAGGTCTCAGCTCCAAAGCTGCCAAGGACGTGCTGGGCTTCCTGCGTGTCGTCCGCTTCGTG CGCATCCTTCGTATCTTCAAGCTGACCCGTCACTTCGTGGGGCTGCGTGTGCTGGGCCACACGCTCCGGG CCAGCACCAACGAATTCTTGTTACTCATCATCTTCCTGGCTCTGGGGGTCCTCATCTTTGCCACCATGAT CTACTATGCCGAACGCATCGGGGCTGATCCTGACGACATCCTGGGCTCCAACCACACCTACTTCAAGAAC ATCCCCATCGGCTTCTGGTGGGCTGTGGTCACCATGACGACACTGGGCTATGGAGACATGTATCCCAAGA CGTGGTCTGGGATGCTGGTTGGGGCACTGTGTGCCCTGGCTGGTGTGCTGACCATTGCCATGCCCGTGCC TGTCATTGTCAACAACTTCGGCATGTACTATTCACTGGCTATGGCCAAGCAGAAATTGCCAAAGAAGAAA AACAAACATATTCCCAGGCCCCCGCAGCCTGGCTCACCCAACTACTGCAAGCCTGACCCCCCGCCTCCAC CCCCACCACACCCCCACCACGGCAGCGGTGGCATAAGCCCACCGCCGCCCATCACCCCTCCTTCCATGGG GGTGAATGTGGCCGGGGCCTACCCACCTGGACCCCACACACACCCCGGGCTGCTCAGGGGTGGTGCTGGG GGCCTGGGAATCATGGGATTGCCTCCTCTGCCAGCCCCTGGTGAGCCCTGCCCATTGGCTCAAGAAGAGG TGATTGAAACCAACAGGGCAGACCCCCGTCCCAATGGAGACCCTGCAGCAGCCGCACTGGCCCATGAGGA CTGCCCTGCCATCGACCAGCCAGCCATGTCTCCAGAAGACAAGAGCCCAATCACTCCCGGAAGCCGGGGT CGCTACAGCCGGGACCGAGCTTGCTTCCTTGTCACAGACTATGCCCCTTCCCCTGATGGCTCCATCCGAA AAGGTTACGAGAAGTCCCGCAGCCTGAGCAGCATTGTGGGCCTGAGCGGGGTGTCCCTGCGCCTCGCGCC CCTCGCCACCCCCCCTGGCTCTCCCCGGGCCACGCGCCGAGCTCCCCCGACCCTGCCCTCCATCCTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226697 representing NM_008422
Red=Cloning site Green=Tags(s) MLSSVCVWSFRGRQGTGKQQPQPVPTPQPPESSPPPLPPPQQQQCSQPGTAASPAGAPLSCGPGGRRAEP CPGLPAVAMGRHGGGGGDSGKIVINVGGVRHETYRSTLRTLPGTRLAGLTEPEAAARFDYDPGTDEFFFD RHPGVFAYVLNYYRTGKLHCPADVCGPLFEEELGFWGIDETDVEACCWMTYRQHRDAEEALDSFEAPDSS ANANANAGGAHDAGLDDEAGAGGGGLDGAGGELKRLCFQDAGGGAGGPAGGAGGAGGTWWRRWQPRVWAL FEDPYSSRAARYVAFASLFFILISITTFCLETHEGFIHISNKTVTQASPIPGAPPENITNVEVETEPFLT YVEGVCVVWFTFEFLMRVTFCPDKVEFLKSSLNIIDCVAILPFYLEVGLSGLSSKAAKDVLGFLRVVRFV RILRIFKLTRHFVGLRVLGHTLRASTNEFLLLIIFLALGVLIFATMIYYAERIGADPDDILGSNHTYFKN IPIGFWWAVVTMTTLGYGDMYPKTWSGMLVGALCALAGVLTIAMPVPVIVNNFGMYYSLAMAKQKLPKKK NKHIPRPPQPGSPNYCKPDPPPPPPPHPHHGSGGISPPPPITPPSMGVNVAGAYPPGPHTHPGLLRGGAG GLGIMGLPPLPAPGEPCPLAQEEVIETNRADPRPNGDPAAAALAHEDCPAIDQPAMSPEDKSPITPGSRG RYSRDRACFLVTDYAPSPDGSIRKGYEKSRSLSSIVGLSGVSLRLAPLATPPGSPRATRRAPPTLPSIL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008422 |
| ORF Size | 2307 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008422.2, NP_032448.2 |
| RefSeq Size | 5072 bp |
| RefSeq ORF | 2310 bp |
| Locus ID | 16504 |
| UniProt ID | Q63959 |
| Gene Summary | Voltage-gated potassium channel that plays an important role in the rapid repolarization of fast-firing brain neurons. The channel opens in response to the voltage difference across the membrane, forming a potassium-selective channel through which potassium ions pass in accordance with their electrochemical gradient. The channel displays rapid activation and inactivation kinetics (PubMed:18539595, PubMed:26997484, PubMed:24218544). It plays a role in the regulation of the frequency, shape and duration of action potentials in Purkinje cells (PubMed:15217387, PubMed:18448641, PubMed:24218544). Required for normal survival of cerebellar neurons, probably via its role in regulating the duration and frequency of action potentials that in turn regulate the activity of voltage-gated Ca(2+) channels and cellular Ca(2+) homeostasis (PubMed:24218544). Required for normal motor function (PubMed:16923152, PubMed:18448641). Plays a role in the reorganization of the cortical actin cytoskeleton and the formation of actin veil structures in neuronal growth cones via its interaction with HAX1 and the Arp2/3 complex (PubMed:26997484).[UniProtKB/Swiss-Prot Function] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221426 | Kcnc3 (untagged) - Mouse potassium voltage gated channel, Shaw-related subfamily, member 3 (Kcnc3), (10ug) |
CNY 9,880.00 |
|
| MR226697 | Kcnc3 (Myc-DDK-tagged) - Mouse potassium voltage gated channel, Shaw-related subfamily, member 3 (Kcnc3) |
CNY 5,632.00 |
|
| MR226697L3 | Lenti ORF clone of Kcnc3 (Myc-DDK-tagged) - Mouse potassium voltage gated channel, Shaw-related subfamily, member 3 (Kcnc3) |
CNY 7,700.00 |
|
| MR226697L4 | Lenti ORF clone of Kcnc3 (mGFP-tagged) - Mouse potassium voltage gated channel, Shaw-related subfamily, member 3 (Kcnc3) |
CNY 7,700.00 |
