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Apaf1 (NM_001042558) Mouse Tagged ORF Clone
CAT＃: MG226529
- TrueORF®
Apaf1 (tGFP-tagged) - Mouse apoptotic peptidase activating factor 1 (Apaf1) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_001042558" in other vectors (4)
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CNY 10,550.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 6230400I06Rik; Apaf-1; Apaf1l; fog; mKIAA0413 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG226529 representing NM_001042558
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGCAAAGGCCCGCAATTGTTTGCTTCAACATAGAGAAGCTTTGGAAAAGGACATCAAAACATCCT ACATCATGGATCACATGATCAGTAATGGCGTCTTGTCAGTGATAGAGGAGGAGAAGGTCAAAAGTCAGGC CACTCAATATCAACGAGCAGCCGCTTTAATTAAAATGATACTTAATAAAGACAACTGTGCCTACATTTCA TTCTACAACGCTCTGCTACACGAGGGCTATAAGGACCTTGCTGCGCTTCTGCAGAGTGGCCTTCCTCTTG TGTCGTCTTCCAGTGGCAAGGACACAGATGGTGGAATAACTTCATTTGTAAGGACAGTGCTGTGTGAAGG TGGAGTACCCCAGAGGCCGGTTATTTTCGTTACTAGAAAGAAGCTGGTTCATGCGATTCAGCAGAAGCTC TGGAAACTGAATGGAGAACCAGGGTGGGTCACCATCTATGGGATGGCAGGCTGCGGCAAGTCTGTGTTAG CTGCGGAAGCCGTTCGAGATCACTCCCTCTTAGAAGGTTGCTTTTCAGGGGGTGTACACTGGGTTTCCAT TGGAAAACAAGACAAATCTGGGCTTCTCATGAAACTGCAGAATCTGTGCATGCGCTTGGACCAAGAAGAG AGTTTCTCTCAGAGGCTTCCACTTAATATTGAGGAGGCCAAAGACCGCCTCCGTGTTCTGATGCTGCGCA AACACCCAAGGTCTCTGTTGATCTTGGATGATGTTTGGGATCCTTGGGTGTTAAAAGCTTTTGACAATCA GTGTCAGATTCTTCTTACAACCAGAGATAAGAGTGTTACAGATTCAGTAATGGGTCCTAAGCATGTTGTC CCTGTGGAGAGTGGTCTAGGGAGAGAGAAAGGACTTGAGATCTTGTCACTTTTTGTTAACATGAAGAAAG AAGATCTGCCAGCGGAGGCTCACAGTATTATAAAGGAATGCAAAGGTTCTCCTCTTGTAGTGTCTTTAAT TGGTGCACTTTTACGTGATTTTCCCAATCGCTGGGCATACTACCTCAGACAGCTTCAGAATAAGCAGTTT AAGAGAATAAGGAAGTCTTCATCTTATGATTATGAGGCTCTAGATGAAGCCATGTCGATAAGTGTTGAAA TGCTCAGAGAAGACATCAAAGACTATTACACAGACCTTTCCATCCTTCAGAAGGACGTCAAGGTACCTAC AAAGGTGTTGTGCGTTCTCTGGGACTTGGAAACGGAAGAAGTTGAAGACATCCTGCAGGAGTTCGTTAAT AAGTCTCTCTTATTCTGTAATCGGAATGGAAAGTCATTTTGTTATTATTTACACGATCTTCAAGTAGATT TTCTTACAGAGAAGAATCGCAGTCAGCTTCAGGATCTGCACAGGAAGATGGTCACTCAGTTTCAGAGGTA TTACCAGCCCCACACGCTGTCTCCAGACCAGGAGGACTGCATGTATTGGTACAACTTCCTAGCCTATCAC ATGGCTAGTGCCAATATGCACAAAGAACTTTGTGCTTTAATGTTTTCCCTGGACTGGATTAAAGCAAAAA CAGAACTTGTCGGCCCTGCCCATCTGATTCACGAGTTCGTGGCATATAGGCATATATTGGATGAAAAGGA TTGTGCAGTCTGTGAGAATTTTCAAGAGTTTTTATCTTTAAATGGACACCTCCTTGGACGACAGCCATTT CCTAATATTGTACAGCTGGGCCTCTGTGAACCAGAAACTTCCGAAGTTTATCGACAAGCAAAGCTGCAGG CCAAGCAGGAGGGGGATACTGGGCGCCTTTACCTGGAATGGATAAACAAAAAAACTATCAAGAATCTGTC CCGCTTAGTCGTCCGCCCCCACACAGATGCTGTTTACCACGCGTGTTTTTCTCAGGATGGCCAGAGAATA GCTTCTTGTGGGGCTGATAAAACCTTACAGGTGTTCAAAGCCGAGACAGGAGAGAAACTTCTTGACATTA AAGCTCATGAAGATGAGGTGCTCTGCTGCGCGTTCTCCTCAGACGACAGTTACATAGCGACCTGCTCAGC GGATAAGAAGGTTAAGATTTGGGATTCTGCGACTGGGAAGCTTGTGCACACCTACGACGAGCACTCGGAG CAAGTCAATTGCTGCCATTTCACCAACAAGAGTAACCACCTTCTCTTGGCCACCGGGTCAAATGATTTCT TCCTCAAGCTCTGGGATTTGAATCAAAAAGAATGTCGAAATACCATGTTTGGTCACACGAACTCAGTCAA CCACTGCAGGTTCTCACCAGACGATGAGCTCTTGGCTAGCTGCTCAGCTGACGGGACTTTAAGGCTTTGG GATGTGAGATCAGCAAACGAGAGGAAAAGCATTAATGTGAAGCGCTTCTTCCTGAGTTCAGAAGACCCTC CAGAGGATGTGGAGGTGATCGTGAAGTGTTGTTCCTGGTCTGCAGATGGTGACAAAATAATAGTGGCAGC AAAAAACAAAGTCCTCCTTTTTGATATTCATACTAGTGGCCTATTGGCAGAGATCCACACAGGCCATCAC AGCACCATCCAGTACTGTGACTTCTCCCCCTATGACCATTTGGCTGTGATTGCCCTGTCTCAGTACTGTG TGGAGTTGTGGAACATAGACTCCCGCCTAAAGGTGGCCGACTGCAGAGGACATTTGAGTTGGGTTCACGG TGTGATGTTTTCTCCCGATGGCTCCTCATTTTTGACAGCTTCTGATGACCAAACAATAAGGGTCTGGGAG ACAAAAAAGGTATGCAAGAACTCTGCCATCGTGCTAAAGCAGGAAATAGACGTCGTGTTTCAAGAGAACG AAACGATGGTCCTTGCAGTTGACAACATAAGAGGCCTGCAACTCATTGCTGGAAAAACAGGCCAGATTGA TTACCTGCCTGAAGCCCAAGTGAGTTGCTGCTGCCTCAGTCCACACCTTGAGTACGTGGCATTCGGAGAT GAAGATGGAGCCATTAAGATTATAGAACTTCCAAACAACAGAGTCTTCAGTTCTGGGGTTGGGCACAAGA AAGCTGTGCGGCACATCCAGTTCACAGCTGATGGGAAGACACTGATTTCAAGTTCTGAAGATTCTGTGAT TCAGGTATGGAATTGGCAGACAGGGGACTATGTATTTTTGCAAGCCCACCAGGAAACGGTAAAGGACTTC AGGCTCCTCCAAGATTCAAGATTGCTTTCTTGGTCATTTGATGGAACGGTGAAGGTGTGGAATGTCATTA CCGGAAGAATAGAAAGAGACTTTACTTGTCATCAGGGCACAGTGCTTTCCTGTGCTATCTCTTCTGATGC GACCAAGTTTTCCTCTACCTCTGCTGATAAGACTGCCAAGATCTGGAGTTTTGACCTCCTTTCCCCTCTT CATGAGCTGAAGGGCCATAATGGCTGTGTCCGCTGCTCTGCCTTCTCGCTGGATGGCATCCTGTTGGCAA CTGGAGATGACAATGGAGAAATCCGGATATGGAATGTCTCAGATGGCCAGCTTCTTCATTCGTGTGCTCC GATCTCGGTAGAGGAAGGAACTGCTACCCACGGCGGCTGGGTAACTGATGTGTGCTTCTCTCCCGACAGT AAAACGCTTGTCTCTGCTGGAGGATATCTCAAGTGGTGGAATGTTGCCACTGGGGACTCCTCACAGACCT TCTACACAAATGGAACAAACCTCAAGAAAATCCACGTGTCCCCTGACTTCAGAACCTATGTGACTGTCGA TAATCTCGGTATTTTATATATTTTACAGGTTTTAGAG AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226529 representing NM_001042558
Red=Cloning site Green=Tags(s) MDAKARNCLLQHREALEKDIKTSYIMDHMISNGVLSVIEEEKVKSQATQYQRAAALIKMILNKDNCAYIS FYNALLHEGYKDLAALLQSGLPLVSSSSGKDTDGGITSFVRTVLCEGGVPQRPVIFVTRKKLVHAIQQKL WKLNGEPGWVTIYGMAGCGKSVLAAEAVRDHSLLEGCFSGGVHWVSIGKQDKSGLLMKLQNLCMRLDQEE SFSQRLPLNIEEAKDRLRVLMLRKHPRSLLILDDVWDPWVLKAFDNQCQILLTTRDKSVTDSVMGPKHVV PVESGLGREKGLEILSLFVNMKKEDLPAEAHSIIKECKGSPLVVSLIGALLRDFPNRWAYYLRQLQNKQF KRIRKSSSYDYEALDEAMSISVEMLREDIKDYYTDLSILQKDVKVPTKVLCVLWDLETEEVEDILQEFVN KSLLFCNRNGKSFCYYLHDLQVDFLTEKNRSQLQDLHRKMVTQFQRYYQPHTLSPDQEDCMYWYNFLAYH MASANMHKELCALMFSLDWIKAKTELVGPAHLIHEFVAYRHILDEKDCAVCENFQEFLSLNGHLLGRQPF PNIVQLGLCEPETSEVYRQAKLQAKQEGDTGRLYLEWINKKTIKNLSRLVVRPHTDAVYHACFSQDGQRI ASCGADKTLQVFKAETGEKLLDIKAHEDEVLCCAFSSDDSYIATCSADKKVKIWDSATGKLVHTYDEHSE QVNCCHFTNKSNHLLLATGSNDFFLKLWDLNQKECRNTMFGHTNSVNHCRFSPDDELLASCSADGTLRLW DVRSANERKSINVKRFFLSSEDPPEDVEVIVKCCSWSADGDKIIVAAKNKVLLFDIHTSGLLAEIHTGHH STIQYCDFSPYDHLAVIALSQYCVELWNIDSRLKVADCRGHLSWVHGVMFSPDGSSFLTASDDQTIRVWE TKKVCKNSAIVLKQEIDVVFQENETMVLAVDNIRGLQLIAGKTGQIDYLPEAQVSCCCLSPHLEYVAFGD EDGAIKIIELPNNRVFSSGVGHKKAVRHIQFTADGKTLISSSEDSVIQVWNWQTGDYVFLQAHQETVKDF RLLQDSRLLSWSFDGTVKVWNVITGRIERDFTCHQGTVLSCAISSDATKFSSTSADKTAKIWSFDLLSPL HELKGHNGCVRCSAFSLDGILLATGDDNGEIRIWNVSDGQLLHSCAPISVEEGTATHGGWVTDVCFSPDS KTLVSAGGYLKWWNVATGDSSQTFYTNGTNLKKIHVSPDFRTYVTVDNLGILYILQVLE SGPTRTRRLE - GFP Tag - V |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001042558 |
| ORF Size | 3747 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001042558.1, NP_001036023.1 |
| RefSeq Size | 6557 bp |
| RefSeq ORF | 3750 bp |
| Locus ID | 11783 |
| UniProt ID | O88879 |
| Gene Summary | Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224055 | Apaf1 (untagged) - Mouse apoptotic peptidase activating factor 1 (Apaf1), transcript variant 1, (10ug) |
CNY 16,060.00 |
|
| MR226529 | Apaf1 (Myc-DDK-tagged) - Mouse apoptotic peptidase activating factor 1 (Apaf1), transcript variant 1 |
CNY 8,696.00 |
|
| MR226529L3 | Lenti ORF clone of Apaf1 (Myc-DDK-tagged) - Mouse apoptotic peptidase activating factor 1 (Apaf1), transcript variant 1 |
CNY 11,500.00 |
|
| MR226529L4 | Lenti ORF clone of Apaf1 (mGFP-tagged) - Mouse apoptotic peptidase activating factor 1 (Apaf1), transcript variant 1 |
CNY 11,500.00 |
