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Sphk1 (NM_025367) Mouse Tagged ORF Clone
CAT＃: MG226229
- TrueORF®
Sphk1 (tGFP-tagged) - Mouse sphingosine kinase 1 (Sphk1) transcript variant 2, (10ug)
ORF Plasmid: DDK
"NM_025367" in other vectors (4)
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CNY 4,180.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 1110006G24Rik; SK; Sk1; Spk1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG226229 representing NM_025367.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAACCAGTAGAATGCCCTCGAGGACTGCTCCCACGGCCATGCAGAGTGCTGGTGCTGCTGAACCCC CAGGGTGGCAAGGGCAAGGCTCTGCAGCTCTTCCAGAGCCGTGTGCAGCCCTTCCTGGAGGAGGCAGAG ATAACCTTTAAACTGATACTCACCGAACGGAAGAACCATGCCAGGGAGCTGGTGTGTGCAGAGGAGTTG GGTCACTGGGACGCCCTGGCAGTCATGTCCGGTGATGGTCTGATGCATGAGGTGGTGAATGGGCTAATG GAACGGCCCGACTGGGAGACTGCCATCCAGAAACCCCTGTGTAGCCTCCCTGGAGGCTCCGGCAATGCG CTGGCAGCTTCTGTGAACCACTATGCTGGGTACGAACAGGTGACTAATGAAGACCTGCTCATCAACTGC ACACTGCTGTTGTGCCGCCGGCGCCTGTCACCCATGAACCTGCTGTCCCTGCACACTGCTTCTGGGCTG CGGCTCTATTCTGTGCTCAGTCTGTCCTGGGGCTTTGTTGCTGACGTGGACCTCGAGAGTGAGAAGTAC AGGCGCTTGGGGGAGATTCGTTTCACAGTGGGCACCTTCTTTCGCCTAGCAAGCCTGCGCATCTACCAA GGCCAACTGGCCTACCTTCCTGTAGGAACTGTGGCCTCTAAGAGACCCGCCTCTACACTGGTGCAGAAG GGCCCCGTCGACACACACCTTGTTCCTCTGGAGGAGCCAGTGCCTTCTCATTGGACTGTGGTACCAGAA CAGGACTTTGTCCTGGTGCTGGTGCTGCTACACACCCACCTGAGCTCCGAGCTGTTTGCAGCACCCATG GGCCGCTGTGAGGCTGGTGTTATGCATCTGTTCTACGTACGTGCGGGGGTGTCAAGGGCTGCGCTGCTG CGCCTCTTCCTGGCCATGCAGAAGGGCAAGCATATGGAACTTGACTGTCCATACCTGGTTCATGTGCCC GTGGTTGCTTTCCGCCTGGAGCCCAGGAGCCAGAGGGGCGTGTTTTCTGTGGATGGAGAGCTGATGGTA TGTGAAGCTGTGCAGGGCCAAGTGCACCCAAACTACCTTTGGATGGTCTGTGGCAGCAGAGATGCCCCA TCCGGCCGGGACTCCCGGCGGGGGCCACCTCCAGAAGAACCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC >Peptide sequence encoded by MG226229
Blue=ORF Red=Cloning site Green=Tag(s) MEPVECPRGLLPRPCRVLVLLNPQGGKGKALQLFQSRVQPFLEEAEITFKLILTERKNHARELVCAEEL GHWDALAVMSGDGLMHEVVNGLMERPDWETAIQKPLCSLPGGSGNALAASVNHYAGYEQVTNEDLLINC TLLLCRRRLSPMNLLSLHTASGLRLYSVLSLSWGFVADVDLESEKYRRLGEIRFTVGTFFRLASLRIYQ GQLAYLPVGTVASKRPASTLVQKGPVDTHLVPLEEPVPSHWTVVPEQDFVLVLVLLHTHLSSELFAAPM GRCEAGVMHLFYVRAGVSRAALLRLFLAMQKGKHMELDCPYLVHVPVVAFRLEPRSQRGVFSVDGELMV CEAVQGQVHPNYLWMVCGSRDAPSGRDSRRGPPPEEP TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_025367 |
| ORF Size | 1146 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq Size | 1763 bp |
| RefSeq ORF | 1149 bp |
| Locus ID | 20698 |
| UniProt ID | Q8CI15 |
| MW | 42.4 kDa |
| Gene Summary | This gene encodes a kinase that phosphorylates sphingosine into sphingosine-1-phosphate, which is involved in cell differentiation, motility, and apoptosis. The encoded protein plays a role in maintaining cellular levels of sphingosine-1-phosphate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC209435 | Sphk1 (untagged) - Mouse sphingosine kinase 1 (Sphk1), transcript variant 2, (10ug) |
CNY 3,656.00 |
|
| MR226229 | Sphk1 (Myc-DDK-tagged) - Mouse sphingosine kinase 1 (Sphk1), transcript variant 2 |
CNY 3,800.00 |
|
| MR226229L3 | Lenti ORF clone of Sphk1 (Myc-DDK-tagged) - Mouse sphingosine kinase 1 (Sphk1), transcript variant 2 |
CNY 5,700.00 |
|
| MR226229L4 | Lenti ORF clone of Sphk1 (mGFP-tagged) - Mouse sphingosine kinase 1 (Sphk1), transcript variant 2 |
CNY 5,700.00 |
