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Sgk1 (NM_001161850) Mouse Tagged ORF Clone
CAT＃: MG225802
- TrueORF®
Sgk1 (tGFP-tagged) - Mouse serum/glucocorticoid regulated kinase 1 (Sgk1) transcript variant 5, (10ug)
ORF Plasmid: DDK
"NM_001161850" in other vectors (4)
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CNY 4,940.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Sg; Sgk |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG225802 representing NM_001161850
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCGAGATGCAGGGCGCGCTGGCTCGGGCTCGGCTCGAGTCCCTGCTCCGGCCCCGCCACAAAAAGC GGGCGGAGGCTCAGAAAAGGAGCGAGTCCGTCCTGCTGAGCGGACTGGCTTTTATGAAACAGAGAAGGAT GGGCCTGAACGATTTTATTCAGAAGATTGCCAGCAACACCTATGCATGCAAACACGCTGAAGTTCAGTCC ATTTTGAAAATGTCCCATCCTCAGGAGCCGGAGCTTATGAACGCTAACCCCTCTCCTCCGCCAAGTCCCT CTCAACAAATCAACCTGGGTCCGTCCTCCAACCCTCACGCCAAACCCTCCGACTTTCACTTCTTGAAAGT GATCGGAAAGGGCAGTTTTGGAAAGGTTCTTCTGGCTAGGCACAAGGCAGAAGAAGTATTCTATGCAGTC AAAGTTTTACAGAAGAAAGCCATCCTGAAGAAGAAAGAGGAGAAGCATATTATGTCAGAGCGGAATGTTC TGTTGAAGAATGTGAAGCACCCTTTCCTGGTGGGCCTTCACTTCTCATTCCAGACCGCTGACAAGCTCTA CTTTGTCCTGGACTACATTAATGGTGGAGAGCTGTTCTACCATCTCCAGAGGGAGCGCTGCTTCCTGGAA CCACGGGCTCGATTCTACGCAGCTGAAATAGCCAGTGCCCTGGGCTATCTGCACTCCCTAAACATCGTTT ATAGAGACTTAAAACCTGAGAATATTCTCCTAGACTCCCAGGGGCACATCGTCCTCACTGACTTTGGGCT CTGCAAAGAGAATATTGAGCATAACGGGACAACATCTACCTTCTGTGGCACGCCTGAGTATCTGGCTCCT GAGGTCCTCCATAAGCAGCCGTATGACCGGACGGTGGACTGGTGGTGTCTTGGGGCTGTCCTGTATGAGA TGCTCTACGGCCTGCCCCCGTTTTATAGCCGGAACACGGCTGAGATGTACGACAATATTCTGAACAAGCC TCTCCAGTTGAAACCAAATATTACAAACTCGGCAAGGCACCTCCTGGAAGGCCTCCTGCAGAAGGACCGG ACCAAGAGGCTGGGTGCCAAGGATGACTTTATGGAGATTAAGAGTCATATTTTCTTCTCTTTAATTAACT GGGATGATCTCATCAATAAGAAGATTACACCCCCATTTAACCCAAATGTGAGTGGGCCCAGTGACCTTCG GCACTTCGATCCCGAGTTTACCGAGGAGCCGGTCCCCAGCTCCATCGGCAGGTCCCCTGACAGCATCCTT GTCACGGCCAGTGTGAAGGAAGCAGCAGAAGCCTTCCTCGGCTTCTCCTATGCACCTCCTGTGGATTCCT TCCTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG225802 representing NM_001161850
Red=Cloning site Green=Tags(s) MGEMQGALARARLESLLRPRHKKRAEAQKRSESVLLSGLAFMKQRRMGLNDFIQKIASNTYACKHAEVQS ILKMSHPQEPELMNANPSPPPSPSQQINLGPSSNPHAKPSDFHFLKVIGKGSFGKVLLARHKAEEVFYAV KVLQKKAILKKKEEKHIMSERNVLLKNVKHPFLVGLHFSFQTADKLYFVLDYINGGELFYHLQRERCFLE PRARFYAAEIASALGYLHSLNIVYRDLKPENILLDSQGHIVLTDFGLCKENIEHNGTTSTFCGTPEYLAP EVLHKQPYDRTVDWWCLGAVLYEMLYGLPPFYSRNTAEMYDNILNKPLQLKPNITNSARHLLEGLLQKDR TKRLGAKDDFMEIKSHIFFSLINWDDLINKKITPPFNPNVSGPSDLRHFDPEFTEEPVPSSIGRSPDSIL VTASVKEAAEAFLGFSYAPPVDSFL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001161850 |
| ORF Size | 1335 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001161850.2, NP_001155322.1 |
| RefSeq Size | 2603 bp |
| RefSeq ORF | 1338 bp |
| Locus ID | 20393 |
| UniProt ID | Q9WVC6 |
| Gene Summary | This gene encodes a serine/threonine protein kinase that plays an important role in cellular stress response. This kinase activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. This enzyme is activated by protein phosphorylation and degraded via the ubiquitination and proteasome pathway. Multiple transcript variants encoding different isoforms have been found for this gene. A pseudogene of this gene was identified on chromosome 12. [provided by RefSeq, Sep 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC215962 | Sgk1 (untagged) - Mouse serum/glucocorticoid regulated kinase 1 (Sgk1), transcript variant 5, (10ug) |
CNY 5,800.00 |
|
| MR225802 | Sgk1 (Myc-DDK-tagged) - Mouse serum/glucocorticoid regulated kinase 1 (Sgk1), transcript variant 5 |
CNY 4,024.00 |
|
| MR225802L3 | Lenti ORF clone of Sgk1 (Myc-DDK-tagged) - Mouse serum/glucocorticoid regulated kinase 1 (Sgk1), transcript variant 5 |
CNY 6,370.00 |
|
| MR225802L4 | Lenti ORF clone of Sgk1 (mGFP-tagged) - Mouse serum/glucocorticoid regulated kinase 1 (Sgk1), transcript variant 5 |
CNY 6,370.00 |
