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Trpv5 (NM_001007572) Mouse Tagged ORF Clone
CAT＃: MG225560
- TrueORF®
Trpv5 (tGFP-tagged) - Mouse transient receptor potential cation channel subfamily V member 5 (Trpv5), (10ug)
ORF Plasmid: DDK
"NM_001007572" in other vectors (4)
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CNY 6,370.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CAT2; D630033B11; ECAC1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG225560 representing NM_001007572
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGCTAAAACTCCTTGGATCCAACTTCAGAAACTTCTCAATTGGTGGGTCAGAGACCAAGACTGGA ACCAGCACGTGGATCAGCTACATATGCTACAGCAAAAAAGTATTTGGGAGTCTCCACTGCTTAGAGCAGC CAAGGAAAATGATATGTGCACACTTAAGAAACTTCAGCATGACCAGAACTGTGACTTCCGGCAAAGAGGA GCTCTGGGAGAGACAGCACTGCATGTAGCTGCCCTCTATGATAACCTGGATGCTGCTATAATGCTGATGG AGGCTGCTCCATACTTGGTCACAGAGTCCACACTGTGTGAGCCATTTGTAGGTCAGACTGCACTGCACAT CGCAGTCATGAACCAGAATGTGAACCTAGTCCGTGCCCTGTTGGCCCGAGGGGCCAGTGCCTCCGCCAGA GCTACAGGCTCCGCCTTCCATCGAAGTTCACACAACCTCATCTACTATGGAGAACACCCTTTGTCCTTTG CTGCCTGTGTGGGTAGTGAGGAGATTGTTAGGTTGCTCATTGAGCATGGAGCTGACATCCGTGCCCAGGA CTCCCTGGGAAATACAGTACTACACATACTTGTCCTGCAGCCCAACAAAACCTTTGCCTGTCAGATGTAC AACCTGCTACTGTCCTATGATGGAGGAGACCACCTGAAGTCCCTGGAGCTTGTGCCCAACAACCAGGGAC TCACCCCCTTCAAGTTGGCTGGGGTGGAAGGCAACACAGTGATGTTTCAACATCTAATGCAGAAACGGAA GCGCATCCAGTGGTCCTTTGGGCCCCTAACATCTTCCCTCTATGACCTCACAGAGATTGACTCCTGGGGT GAGGAGCTGTCCTTTCTGGAGCTTGTGGTTTCCTCCAAGAAGAAAGAGGCTCGACAGATTCTAGAACAGA CCCCAGTGAAGGAGCTGGTGAGCCTGAAGTGGAAGAAATATGGACAGCCTTATTTCTGCCTCCTGGGTGC TCTGTACATCTTCTACATGGTCTGCTTCACCACATGCTGTGTTTACCGCCCCCTCAAGTTCCGAGATGCC AACCGTACACATGTTCGAGATAACACCATCATGGAACAGAAATCACTACAGGAAGCATATGTGACTTACC AGGATAAGATCCGGCTGGTCGGGGAGTTGGTGACAGTCATTGGGGCTGTAATTATTCTACTCCTAGAGAT CCCAGACATCTTCAGAGTTGGTGCCTCTCGCTACTTTGGACAAACAGTTCTTGGGGGCCCATTCCATGTC ATCATCATTACATATGCCTCCCTTGTGCTACTGACTATGGCAATGAGGCTTACCAATGTGAATGGCGAGG TAGTGCCCATGTCCATGGCCCTGGTGCTGGGCTGGTGTAGTGTCATGTACTTCGCCCGAGGATTCCAGAT GCTGGGCCCCTTCACAATCATGATTCAGAAGATGATTTTTGGAGACCTACTGCGCTTCTGCTGGCTAATG GCTATGGTCATCCTGGGATTTGCCTCAGCCTTCTATATCATTTTCCAGACAGAGGATCCAGATAATCTGG GGGAATTCTCCGACTACCCCACAGCAATGTTCAGCACCTTTGAGCTGTTCCTCACCATCATTGACGGACC TGCCAATTACAGAGTGGATCTGCCCTTCATGTACAGTGTTACCTATGCCACCTTTGCCATCATCGCCACA CTGCTCATGCTCAACTTGTTCATTGCCATGATGGGCGACACTCACTGGCGAGTGGCCCAGGAGAGGGACG AGCTCTGGAGAGCACAAGTTGTAGCCACCACAGTGATGCTGGAGAGGAAAATGCCTCGTTTCCTGTGGCC TCGCTCTGGTATCTGTGGTTGCGAATACGGCCTGGGAGACCGTTGGTTCTTACGGGTTGAACACCACCAG GAGCAGAATCCTTATCGAGTACTTCGATATGTGGAAGCTTTCAAATCTTCAGACAAAGAGGAAGTACAAG AGCAGCTATCTGAGAAACAACCTTCTGGGACTGAGACTGGGACTTTAGCTAGAGGCTCTGTGGTTCTCCA AACACCTCCTTTGTCCAGGACTACATCCCTAAGCAGCAATAGCCACCGTGGATGGGAAATTCTCCGTCGA AACACCCTGGGACATTTGAATCTTGGACTAGACCCTGGTGAAGGGGATGGGGAAGAGATCTACCAGTTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG225560 representing NM_001007572
Red=Cloning site Green=Tags(s) MGAKTPWIQLQKLLNWWVRDQDWNQHVDQLHMLQQKSIWESPLLRAAKENDMCTLKKLQHDQNCDFRQRG ALGETALHVAALYDNLDAAIMLMEAAPYLVTESTLCEPFVGQTALHIAVMNQNVNLVRALLARGASASAR ATGSAFHRSSHNLIYYGEHPLSFAACVGSEEIVRLLIEHGADIRAQDSLGNTVLHILVLQPNKTFACQMY NLLLSYDGGDHLKSLELVPNNQGLTPFKLAGVEGNTVMFQHLMQKRKRIQWSFGPLTSSLYDLTEIDSWG EELSFLELVVSSKKKEARQILEQTPVKELVSLKWKKYGQPYFCLLGALYIFYMVCFTTCCVYRPLKFRDA NRTHVRDNTIMEQKSLQEAYVTYQDKIRLVGELVTVIGAVIILLLEIPDIFRVGASRYFGQTVLGGPFHV IIITYASLVLLTMAMRLTNVNGEVVPMSMALVLGWCSVMYFARGFQMLGPFTIMIQKMIFGDLLRFCWLM AMVILGFASAFYIIFQTEDPDNLGEFSDYPTAMFSTFELFLTIIDGPANYRVDLPFMYSVTYATFAIIAT LLMLNLFIAMMGDTHWRVAQERDELWRAQVVATTVMLERKMPRFLWPRSGICGCEYGLGDRWFLRVEHHQ EQNPYRVLRYVEAFKSSDKEEVQEQLSEKQPSGTETGTLARGSVVLQTPPLSRTTSLSSNSHRGWEILRR NTLGHLNLGLDPGEGDGEEIYQF TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001007572 |
| ORF Size | 2169 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001007572.2, NP_001007573.1 |
| RefSeq Size | 2601 bp |
| RefSeq ORF | 2172 bp |
| Locus ID | 194352 |
| UniProt ID | P69744 |
| Gene Summary | Constitutively active calcium selective cation channel thought to be involved in Ca(2+) reabsorption in kidney and intestine (PubMed:12077127, PubMed:14679186). Required for normal Ca(2+) reabsorption in the kidney distal convoluted tubules (PubMed:14679186). The channel is activated by low internal calcium level and the current exhibits an inward rectification. A Ca(2+)-dependent feedback regulation includes fast channel inactivation and slow current decay (PubMed:12077127). Heteromeric assembly with TRPV6 seems to modify channel properties. TRPV5-TRPV6 heteromultimeric concatemers exhibit voltage-dependent gating (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221118 | Trpv5 (untagged) - Mouse transient receptor potential cation channel, subfamily V, member 5 (Trpv5), (10ug) |
CNY 9,310.00 |
|
| MR225560 | Trpv5 (Myc-DDK-tagged) - Mouse transient receptor potential cation channel, subfamily V, member 5 (Trpv5) |
CNY 5,296.00 |
|
| MR225560L3 | Lenti ORF clone of Trpv5 (Myc-DDK-tagged) - Mouse transient receptor potential cation channel, subfamily V, member 5 (Trpv5) |
CNY 7,700.00 |
|
| MR225560L4 | Lenti ORF clone of Trpv5 (mGFP-tagged) - Mouse transient receptor potential cation channel, subfamily V, member 5 (Trpv5) |
CNY 7,700.00 |
