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Papss2 (NM_011864) Mouse Tagged ORF Clone
CAT＃: MG225151
- TrueORF®
Papss2 (tGFP-tagged) - Mouse 3'-phosphoadenosine 5'-phosphosulfate synthase 2 (Papss2), (10ug)
ORF Plasmid: DDK
"NM_011864" in other vectors (4)
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CNY 6,080.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 1810018P12Rik; AI159688; Atpsk2; AtpsU2; bm; Sk2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG225151 representing NM_011864
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGCAAATTTCAAAATGAACCATAAAAGAGACCAGCAAAAATCCACCAATGTGGTCTACCAGGCCC ATCATGTGAGCAGGAACAAGAGAGGACAAGTGGTTGGAACCAGGGGAGGATTCCGAGGATGTACCGTGTG GCTAACAGGTCTCTCTGGTGCTGGGAAAACAACCATAAGCTTTGCTTTGGAAGAGTACCTTGTATCTCAC GCCATCCCATGTTACTCTCTGGATGGGGACAATGTCCGTCATGGCCTTAATAAGAACCTGGGATTCTCTG CGGGGGACCGAGAAGAGAATATCCGCCGGATCGCGGAGGTGGCCAGGCTCTTTGCCGACGCCGGCCTGGT TTGCATCACCAGCTTTATCTCTCCTTTTGCAAAGGATCGTGAGAATGCCCGAAAAATCCACGAATCAGCA GGACTCCCATTCTTTGAGATCTTTGTAGATGCGCCTTTAAATATCTGTGAAAGCCGAGACGTAAAAGGAC TCTACAAACGAGCCCGAGCAGGAGAGATTAAAGGGTTTACAGGCATCGATTCTGACTATGAGAAACCTGA AACTCCAGAGTGTGTGCTGAAGACCAACCTGTCTTCAGTAAGCGACTGTGTGCAACAGGTGGTGGAACTT TTGCAGGAGCAGAACATTGTACCCCACACCACCATCAAAGGCATCCACGAACTCTTTGTGCCAGAAAACA AAGTCGATCAAATCCGAGCTGAGGCAGAGACTCTCCCATCACTACCAATTACCAAGCTGGATCTGCAGTG GGTGCAGATTCTGAGTGAAGGCTGGGCCACTCCCCTCAAAGGCTTTATGCGGGAGAAGGAGTACTTGCAA ACTCTACACTTCGACACTCTACTGGACGGCGTGGTTCCCCGTGATGGAGTCATCAACATGAGTATTCCCA TTGTATTGCCCGTTTCTGCGGATGACAAGGCACGGCTCGAAGGGTGCAGCAAATTTGCCTTGATGTACGA AGGTCGGAGGGTCGCTCTATTACAGGACCCTGAATTCTATGAGCATAGGAAAGAGGAGCGCTGTTCTCGT GTGTGGGGAACAGCCACTGCAAAGCACCCCCATATCAAAATGGTGATGGAAAGTGGGGACTGGCTTGTTG GTGGAGACCTACAGGTGCTAGAGAGAATAAGGTGGGACGATGGGCTGGACCAATACCGCCTTACGCCTCT GGAGCTCAAACAGAAGTGTAAAGACATGAATGCTGATGCCGTGTTTGCATTCCAGTTGCGCAATCCTGTC CACAATGGTCATGCCCTCCTGATGCAGGACACCCGCCGCAGGCTCCTGGAGAGGGGTTACAAGCACCCAG TCCTCCTGCTCCACCCTCTTGGGGGCTGGACCAAGGACGATGACGTACCTCTGGAATGGAGGATGAAACA GCATGCAGCTGTACTGGAGGAAAGGGTCCTGGATCCCAAGTCAACTATTGTTGCCATCTTTCCATCTCCT ATGTTATATGCTGGTCCCACAGAGGTCCAGTGGCATTGCAGATGCCGGATGATTGCAGGAGCCAATTTCT ACATTGTGGGTAGGGATCCCGCAGGAATGCCCCATCCTGAGACAAAGAAAGACCTATATGAACCCACCCA CGGGGGCAAGGTCTTGAGTATGGCCCCTGGCCTTACCTCTGTGGAAATAATTCCGTTCCGAGTGGCTGCC TACAATAAAATTAAAAAGGCCATGGACTTTTATGATCCAGCAAGGCACGAGGAGTTTGACTTCATCTCAG GAACTCGCATGAGGAAGCTCGCCCGGGAAGGAGAAGATCCCCCAGATGGCTTCATGGCCCCGAAAGCGTG GAAAGTGTTGACAGATTACTACAGGTCTCTGGAGAAGACCAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG225151 representing NM_011864
Red=Cloning site Green=Tags(s) MSANFKMNHKRDQQKSTNVVYQAHHVSRNKRGQVVGTRGGFRGCTVWLTGLSGAGKTTISFALEEYLVSH AIPCYSLDGDNVRHGLNKNLGFSAGDREENIRRIAEVARLFADAGLVCITSFISPFAKDRENARKIHESA GLPFFEIFVDAPLNICESRDVKGLYKRARAGEIKGFTGIDSDYEKPETPECVLKTNLSSVSDCVQQVVEL LQEQNIVPHTTIKGIHELFVPENKVDQIRAEAETLPSLPITKLDLQWVQILSEGWATPLKGFMREKEYLQ TLHFDTLLDGVVPRDGVINMSIPIVLPVSADDKARLEGCSKFALMYEGRRVALLQDPEFYEHRKEERCSR VWGTATAKHPHIKMVMESGDWLVGGDLQVLERIRWDDGLDQYRLTPLELKQKCKDMNADAVFAFQLRNPV HNGHALLMQDTRRRLLERGYKHPVLLLHPLGGWTKDDDVPLEWRMKQHAAVLEERVLDPKSTIVAIFPSP MLYAGPTEVQWHCRCRMIAGANFYIVGRDPAGMPHPETKKDLYEPTHGGKVLSMAPGLTSVEIIPFRVAA YNKIKKAMDFYDPARHEEFDFISGTRMRKLAREGEDPPDGFMAPKAWKVLTDYYRSLEKTN TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011864 |
| ORF Size | 1863 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011864.2 |
| RefSeq Size | 3635 bp |
| RefSeq ORF | 1866 bp |
| Locus ID | 23972 |
| UniProt ID | O88428 |
| Gene Summary | Bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway. The first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate (PAPS: activated sulfate donor used by sulfotransferase). In mammals, PAPS is the sole source of sulfate; APS appears to be only an intermediate in the sulfate-activation pathway. May have an important role in skeletogenesis during postnatal growth.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC219817 | Papss2 (untagged) - Mouse 3'-phosphoadenosine 5'-phosphosulfate synthase 2 (Papss2), transcript variant 1, (10ug) |
CNY 7,980.00 |
|
| MR225151 | Papss2 (Myc-DDK-tagged) - Mouse 3'-phosphoadenosine 5'-phosphosulfate synthase 2 (Papss2), transcript variant 1 |
CNY 4,632.00 |
|
| MR225151L3 | Lenti ORF clone of Papss2 (Myc-DDK-tagged) - Mouse 3'-phosphoadenosine 5'-phosphosulfate synthase 2 (Papss2), transcript variant 1 |
CNY 7,410.00 |
|
| MR225151L4 | Lenti ORF clone of Papss2 (mGFP-tagged) - Mouse 3'-phosphoadenosine 5'-phosphosulfate synthase 2 (Papss2), transcript variant 1 |
CNY 7,410.00 |
