Tbata (NM_001017433) Mouse Tagged ORF Clone

CAT#: MG224727

  • TrueORF®

Tbata (tGFP-tagged) - Mouse thymus brain and testes associated (Tbata) transcript variant 1, (10ug)

ORF Plasmid: DDK tGFP



  "NM_001017433" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 3,140.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms 1700021K02Rik; AI428928; S; Spatial; Titest
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG224727 representing NM_001017433
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACTACAGAGGTGAACCAGCTGTCTGAGCATCCTCTAGTGAGTCCAAAGGCAGAGCCCCAGCCAGAGA
CGAAGCCGGAGAACCTTCCAAGGAGCCACGGGGATGTTGGGCTCCAGAAAGAGACTGTGGTCCCAGGCAT
TGTGGATTTCGAGCTGATCCATGAGGAGCTGAAGACCACAAAGCCCCAAACATCACAACCAACACCCAGT
GCCTACCGCTTTGGACGCCTAAGCCACCATTCCTTCTTCTCGAGGCACCACCCCCAACCACAGCGAGTGA
CTCATATCCAAGTTACAGGAAGAGAGGACCTGGAGCACTCCCTGCCCCTCACCACCTCTTTCCAGCTCCT
TCAAGCTCCTGGGGTCCAGCCCATGGATCTCACTCCCTCTGCAGATATCGCTGGGAAGCCTGTCTGCGTG
GTCAGGGACGAGTTCTCTCTGTCGGCCTTGACTCAGCCCACATTCTTATCCCGCTGTCTGATGGGGATGC
CCACCATCTCTGTCCCCATTGGGGATCCACAGTCCAATCGGAACCCCCAGCTTTCTACTTCTGACACCTG
GAGGAAGAAACTGAAGGACCTGGCTTCCCGAGTGACTGTCTTCACTAAGGAAATCCAGCCAAAGCCCGAT
GAGCAGAAGGAAGAGCCACCTCTGAGGGAACCTCCTCCGAGAGAGCAGGGGGCCAAATACTCAGCTGAGA
CCGGTAGGCTTATCCCTGCTTCCAGCCAAGCCCTCACCCGTCGCAACCGCCAGGGCCAGCGGGTCCACCC
TTCTAGCAAAGATGGAGGAGTCCAAGCCTCCATTCTGCAGGACCAGGAGCTGCTGATTTTGGAGCTCCTT
TGTCAAATTCTACAAACAGATTCTCTAAGGGCTATTCAGTTCTGGCTGCTTTATGCTCCATCAAAAGAAA
AAGATTTAGCTCTGGGGCTTCTGCAAACTGCCGTGGCTCAGCTTATCCCCCAGCCCCTTTCCTCCATCCC
AGCAGAGAAGCTCTGGAACCATCTCCAAGAGCTTCAAGAGCCTCAAGAGACACAAGAGGCAGCCTACAGT
CCATCCCTGAAGAAGACTAGGTCACCACCTCTACCCAAAACAGACAAACCAGAGTACATAGGCAAAGCCC
AAGTCCTCCTGGTGCATCCCAGCGAGGACCCAGAGGAGAAAACGACCAAGGCTGAAAGC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG224727 representing NM_001017433
Red=Cloning site Green=Tags(s)

MTTEVNQLSEHPLVSPKAEPQPETKPENLPRSHGDVGLQKETVVPGIVDFELIHEELKTTKPQTSQPTPS
AYRFGRLSHHSFFSRHHPQPQRVTHIQVTGREDLEHSLPLTTSFQLLQAPGVQPMDLTPSADIAGKPVCV
VRDEFSLSALTQPTFLSRCLMGMPTISVPIGDPQSNRNPQLSTSDTWRKKLKDLASRVTVFTKEIQPKPD
EQKEEPPLREPPPREQGAKYSAETGRLIPASSQALTRRNRQGQRVHPSSKDGGVQASILQDQELLILELL
CQILQTDSLRAIQFWLLYAPSKEKDLALGLLQTAVAQLIPQPLSSIPAEKLWNHLQELQEPQETQEAAYS
PSLKKTRSPPLPKTDKPEYIGKAQVLLVHPSEDPEEKTTKAES

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001017433
ORF Size 1179 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001017433.2, NP_001017433.1
RefSeq Size 1545 bp
RefSeq ORF 1182 bp
Locus ID 65971
UniProt ID Q7TSD4
Gene Summary This gene encodes a putative transcription factor that is highly expressed in thymic cortical stromal cells, and may be involved in T-cell development. Its expression is developmentally regulated in the testis, where it is restricted to the haploid round spermatids during spermatogenesis, and thus this gene may also have a role in the control of male germ cell development. Alternative splicing of this gene results in two sets of transcript variants: the variants containing 5 additional exons at the 3' end encode long isoforms that are highly expressed in the testis, while the variants lacking the 3' end exons encode short isoforms that are highly expressed in the thymus. Most of the transcripts encoding the short isoforms have been shown to initiate translation from non-AUG (CUG) start sites. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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