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Cacna2d1 (NM_009784) Mouse Tagged ORF Clone
CAT＃: MG222812
- TrueORF®
Cacna2d1 (tGFP-tagged) - Mouse calcium channel voltage-dependent alpha2/delta subunit 1 (Cacna2d1) transcript variant e, (10ug)
ORF Plasmid: DDK
"NM_009784" in other vectors (4)
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CNY 11,880.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Ca(v)alpha2delta1; Cac; Cacna2; Cchl; Cchl2a |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG222812 representing NM_009784
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCTGGCTGCCTGCTGGCCTTGACTCTGACACTTTTCCAATCTGGGCTGATCGGCCCCTCGAGCG AGGAGCCCTTCCCTTCGCCCGTCACTATCAAGTCATGGGTGGACAAGATGCAAGAAGACCTTGTCACACT GGCAAAAACAGCAAGTGGAGTGACTCAGCTTGCTGATATTTATGAAAAATACCAAGATTTGTATACTGTG GAGCCCAACAATGCACGCCAACTGGTTGAAATTGCAGCCCGAGACATTGAGAAGCTTCTAAGCAACAGAT CTAAGGCCCTGGTGCGCCTGGCTATGGAAGCAGAGAAAGTCCAAGCAGCCCACCAATGGAGGGAAGATTT TGCAAGCAATGAAGTTGTCTACTATAATGCTAAGGATGATCTTGATCCTGAAAGAAATGAGAGTGAGCCA GGCAGCCAACGGATTAAACCTGTTTTTATTGAAGATGCTAATTTTGGACGTCAGATATCCTATCAGCATG CAGCGGTCCATATTCCCACGGACATCTATGAGGGCTCAACCATAGTGTTAAACGAACTCAACTGGACAAG TGCCTTAGATGAAGTATTCAAAAGAAATCGAGACGAAGACCCTACACTGCTGTGGCAAGTGTTTGGCAGC GCCACTGGCCTGGCCCGATATTATCCAGCTTCTCCATGGGTGGATAATAGTAGAACTCCAAACAAGATTG ATCTATATGATGTACGCAGAAGACCATGGTACATCCAGGGAGCTGCATCCCCCAAGGACATGCTCATTCT GGTGGACGTGAGTGGAAGTGTGAGCGGATTGACTCTGAAACTCATCCGAACATCTGTCTCCGAGATGTTA GAAACCCTCTCTGATGATGATTTCGTGAATGTAGCTTCATTTAACAGCAACGCTCAGGATGTAAGCTGTT TCCAGCACCTGGTTCAAGCGAATGTAAGAAATAAGAAGGTGTTGAAAGATGCCGTGAATAACATTACAGC AAAGGGGATCACAGATTACAAGAAAGGCTTTAGCTTTGCCTTCGAACAGCTACTTAATTATAATGTTTCC AGAGCTAATTGCAATAAGATTATCATGTTATTCACGGATGGAGGAGAAGAGAGAGCCCAGGAGATATTTG CCAAATACAATAAAGACAAAAAAGTCCGTGTGTTTACATTTTCCGTCGGTCAACATAATTATGACAGAGG ACCTATTCAGTGGATGGCTTGTGAAAATAAAGGTTACTATTATGAGATTCCCTCCATTGGTGCAATAAGA ATCAATACTCAGGAATATCTAGATGTTCTGGGAAGACCAATGGTTTTAGCTGGTGACAAAGCGAAGCAAG TTCAATGGACAAATGTGTATTTGGACGCCCTGGAACTGGGACTTGTCATTACTGGAACTCTACCAGTCTT CAACGTCACTGGCCAATCTGAAAATAAGACAAACTTGAAGAACCAGTTGATTCTTGGTGTGATGGGCGTT GATGTGTCTCTGGAAGATATCAAGAGATTGACACCACGTTTTACACTCTGTCCCAATGGCTACTATTTTG CAATTGATCCTAATGGCTATGTCTTATTGCATCCAAATCTTCAGCCAAAGAACCCCAAATCTCAGGAGCC AGTCACACTGGATTTTCTCGATGCTGAGTTAGAGAATGAAATTAAAGTGGAGATTCGAAATAAAATGATA GATGGAGAAAGTGGAGAAAAAACGTTCAGAACTCTGGTCAAGTCTCAAGATGAGAGATACATTGACAAAG GAAATCGAACATACACATGGACGCCTGTCAATGGCACAGATTACAGTTTGGCCTTGGTATTGCCAACCTA CAGTTTTTACTATATAAAAGCCAAACTAGAAGAGACAATAACTCAGGCCAGATATTCAGAAACCCTGAAG CCAGACAATTTTGAAGAATCTGGCTATACTTTCATAGCACCAAGGGAATACTGCAATGATCTTAAACCTT CAGATAATAACACTGAATTTCTTTTGAATTTCAATGAATTTATTGATAGGAAAACTCCAAACAACCCCTC CTGTAATACAGATTTGATTAATAGAATCTTGCTGGATGCAGGTTTTACAAATGAACTTGTCCAAAATTAT TGGAGTAAGCAGAAAAATATCAAAGGAGTGAAGGCACGCTTTGTGGTGACAGACGGTGGGATTACGAGAG TTTATCCCAAAGAGGCCGGAGAAAATTGGCAAGAGAACCCAGAGACGTACGAGGACAGCTTCTACAAACG GAGCCTAGATAACGATAACTACGTTTTCACTGCGCCCTACTTTAACAAAAGTGGACCTGGTGCGTATGAA TCTGGAATTATGGTAAGCAAAGCAGTAGAACTGTATATCCAAGGAAAACTTCTTAAGCCCGCAGTTGTGG GAATTAAAATTGATGTAAATTCTTGGATAGAAAATTTTACCAAAACTTCAATCAGGGATCCGTGTGCTGG TCCAGTTTGTGACTGCAAAAGAAACAGTGATGTAATGGACTGTGTCATTCTAGATGATGGTGGATTTCTT TTGATGGCAAATCACGATGATTACACTAATCAGATTGGACGTTTTTTTGGAGAGATTGACCCAAGCATGA TGAGACACCTGGTTAATATATCACTTTATGCATTCAACAAATCATATGACTATCAGTCCGTGTGCGATCC AGGGGCAGCACCAAAGCAGGGGGCAGGACATCGCTCAGCATATGTGCCATCGATTGCAGATATACTGCAG ATTGGCTGGTGGGCCACCGCTGCCGCCTGGTCTATTCTCCAGCAGCTGCTCTTGAGTTTGACATTTCCAC GGCTCCTTGAGGCAGTTGAGATGGAGGAAGATGACTTCACAGCCTCCCTGTCTAAGCAGAGCTGCATCAC AGAACAAACCCAGTACTTCTTCAAGAACGATACTAAATCATTCAGTGGTTTACTGGACTGTGGAAACTGT TCCAGGATCTTTCATGTAGAGAAACTTATGAACACCAACTTAGTATTCATAATGGTGGAGAGCAAAGGGA CATGTCCGTGTGACACGCGGCTGCTCATGCAAGCGGAACAGACTTCTGATGGTCCAGATCCTTGCGACAT GGTCAAGCAGCCCAGATACCGAAAAGGACCTGATGTCTGCTTTGATAATAATGTGCTGGAGGATTATACT GACTGTGGTGGTGTTTCTGGGTTAAACCCTTCCTTATGGTCTATCTTTGGACTCCAGTTTATACTCCTTT GGCTGGTATCTGGCAGCAGACACTACCTACTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG222812 representing NM_009784
Red=Cloning site Green=Tags(s) MAAGCLLALTLTLFQSGLIGPSSEEPFPSPVTIKSWVDKMQEDLVTLAKTASGVTQLADIYEKYQDLYTV EPNNARQLVEIAARDIEKLLSNRSKALVRLAMEAEKVQAAHQWREDFASNEVVYYNAKDDLDPERNESEP GSQRIKPVFIEDANFGRQISYQHAAVHIPTDIYEGSTIVLNELNWTSALDEVFKRNRDEDPTLLWQVFGS ATGLARYYPASPWVDNSRTPNKIDLYDVRRRPWYIQGAASPKDMLILVDVSGSVSGLTLKLIRTSVSEML ETLSDDDFVNVASFNSNAQDVSCFQHLVQANVRNKKVLKDAVNNITAKGITDYKKGFSFAFEQLLNYNVS RANCNKIIMLFTDGGEERAQEIFAKYNKDKKVRVFTFSVGQHNYDRGPIQWMACENKGYYYEIPSIGAIR INTQEYLDVLGRPMVLAGDKAKQVQWTNVYLDALELGLVITGTLPVFNVTGQSENKTNLKNQLILGVMGV DVSLEDIKRLTPRFTLCPNGYYFAIDPNGYVLLHPNLQPKNPKSQEPVTLDFLDAELENEIKVEIRNKMI DGESGEKTFRTLVKSQDERYIDKGNRTYTWTPVNGTDYSLALVLPTYSFYYIKAKLEETITQARYSETLK PDNFEESGYTFIAPREYCNDLKPSDNNTEFLLNFNEFIDRKTPNNPSCNTDLINRILLDAGFTNELVQNY WSKQKNIKGVKARFVVTDGGITRVYPKEAGENWQENPETYEDSFYKRSLDNDNYVFTAPYFNKSGPGAYE SGIMVSKAVELYIQGKLLKPAVVGIKIDVNSWIENFTKTSIRDPCAGPVCDCKRNSDVMDCVILDDGGFL LMANHDDYTNQIGRFFGEIDPSMMRHLVNISLYAFNKSYDYQSVCDPGAAPKQGAGHRSAYVPSIADILQ IGWWATAAAWSILQQLLLSLTFPRLLEAVEMEEDDFTASLSKQSCITEQTQYFFKNDTKSFSGLLDCGNC SRIFHVEKLMNTNLVFIMVESKGTCPCDTRLLMQAEQTSDGPDPCDMVKQPRYRKGPDVCFDNNVLEDYT DCGGVSGLNPSLWSIFGLQFILLWLVSGSRHYLL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009784 |
| ORF Size | 3252 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009784.2, NP_033914.1 |
| RefSeq Size | 7415 bp |
| RefSeq ORF | 3255 bp |
| Locus ID | 12293 |
| UniProt ID | O08532 |
| Gene Summary | This gene encodes a regulatory component of the voltage-dependent calcium channel complex. The product of this gene is a proprotein that is proteolytically processed into alpha-2 and delta subunits, which are linked by a disulfide bond. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223473 | Cacna2d1 (untagged) - Mouse calcium channel, voltage-dependent, alpha2/delta subunit 1 (Cacna2d1), transcript variant e, (10ug) |
CNY 13,870.00 |
|
| MR222812 | Cacna2d1 (Myc-DDK-tagged) - Mouse calcium channel, voltage-dependent, alpha2/delta subunit 1 (Cacna2d1), transcript variant e |
CNY 10,830.00 |
|
| MR222812L3 | Lenti ORF clone of Cacna2d1 (Myc-DDK-tagged) - Mouse calcium channel, voltage-dependent, alpha2/delta subunit 1 (Cacna2d1), transcript variant e |
CNY 12,730.00 |
|
| MR222812L4 | Lenti ORF clone of Cacna2d1 (mGFP-tagged) - Mouse calcium channel, voltage-dependent, alpha2/delta subunit 1 (Cacna2d1), transcript variant e |
CNY 12,730.00 |
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