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Crem (NM_001110857) Mouse Tagged ORF Clone
CAT＃: MG222544
- TrueORF®
Crem (tGFP-tagged) - Mouse cAMP responsive element modulator (Crem) transcript variant 5, (10ug)
ORF Plasmid: DDK
"NM_001110857" in other vectors (4)
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CNY 2,760.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | IC; ICER; ICERI |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG222544 representing NM_001110857
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCAAATGTGGCAGGAAAAAGTATATGAGGACAAATGTAAGGCAAATGACCATGGAAACAGTTGAAT CACAGCAGGATCGAAGTGTAACACGTTCTGTGGCAGAGCATAGCTCTGCTCATATGCAGACTGGTCAAAT TTCTGTTCCTACTCTAGCTCAGGTAGCAACAATTGCAGAGACAGATGATTCTGCAGACTCAGAAGTAATT GATTCGCATAAACGTAGAGAAATTCTTTCACGAAGACCCTCATATAGAAAAATACTGAATGAACTTTCCT CTGATGTGCCTGGTATTCCCAAGATTGAAGAAGAAAAATCAGAGGAAGAAGGGACACCACCTAACATTGC TACCATGGCAGTACCAACTAGCATATATCAGACTAGCACGGGGCAATACAATGAGGAGACTGACCTTGCC CCAAGTCACATGGCTGCTGCCACAGGTGACATGCCAACTTACCAGATCCGAGCTCCTACTACTGCTTTGC CACAAGGTGTGGTGATGGCTGCCTCACCAGGAAGCCTGCACAGTCCCCAGCAACTAGCAGAAGAAGCAAC TCGCAAGCGGGAGCTGAGGCTGATGAAAAACAGGGAAGCTGCTAAAGAATGTCGACGTCGAAAGAAAGAG TATGTGAAGTGTCTTGAGAGTCGAGTCGCAGTGCTGGAAGTTCAGAACAAGAAGCTTATAGAGGAGCTTG AAACTTTGAAAGACATTTGCTCTCCCAAAACAGAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG222544 representing NM_001110857
Red=Cloning site Green=Tags(s) MSKCGRKKYMRTNVRQMTMETVESQQDRSVTRSVAEHSSAHMQTGQISVPTLAQVATIAETDDSADSEVI DSHKRREILSRRPSYRKILNELSSDVPGIPKIEEEKSEEEGTPPNIATMAVPTSIYQTSTGQYNEETDLA PSHMAAATGDMPTYQIRAPTTALPQGVVMAASPGSLHSPQQLAEEATRKRELRLMKNREAAKECRRRKKE YVKCLESRVAVLEVQNKKLIEELETLKDICSPKTD TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001110857 |
| ORF Size | 735 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001110857.2, NP_001104327.1 |
| RefSeq Size | 2236 bp |
| RefSeq ORF | 738 bp |
| Locus ID | 12916 |
| UniProt ID | P27699 |
| Gene Summary | This gene encodes a basic-leucine zipper domain-containing protein that localizes to gene promoters, where it binds to the cyclic AMP response element (CRE). Different protein isoforms encoded by this gene may function as either activators or repressors of transcription. Activity of this gene is important in multiple developmental processes, including spermatogenesis. Mutation of this gene causes male infertility. Alternative splicing and promoter usage result in multiple transcript variants for this gene. [provided by RefSeq, Oct 2012] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC208325 | Crem (untagged) - Mouse cAMP responsive element modulator (Crem), transcript variant 5, (10ug) |
CNY 3,990.00 |
|
| MR222544 | Crem (Myc-DDK-tagged) - Mouse cAMP responsive element modulator (Crem), transcript variant 5 |
CNY 2,470.00 |
|
| MR222544L3 | Lenti ORF clone of Crem (Myc-DDK-tagged) - Mouse cAMP responsive element modulator (Crem), transcript variant 5 |
CNY 4,370.00 |
|
| MR222544L4 | Lenti ORF clone of Crem (mGFP-tagged) - Mouse cAMP responsive element modulator (Crem), transcript variant 5 |
CNY 4,370.00 |
