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Spag9 (NM_001025428) Mouse Tagged ORF Clone
CAT＃: MG221375
- TrueORF®
Spag9 (tGFP-tagged) - Mouse sperm associated antigen 9 (Spag9) transcript variant 2, (10ug)
ORF Plasmid: DDK
"NM_001025428" in other vectors (3)
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CNY 12,830.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 3110018C07Rik; 4733401I23Rik; 4831406C20Rik; AW552012; Jip4; JLP; JSAP2; JSAP2a |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG221375 representing NM_001025428
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATCCTGGATGCATGCTGCTGTTTGTGTTTGGCTTTGTTGGCGGGGCGGTGGTCATTAATTCTGCGA TCTTAGTATCTCTCTCTGTTTTGCTGCTTGTGCACTTTTCTATTTCTACCGGGGTGCCAGCGCTGACGCA GAACCTACCAAGGATACTCAGAAAAGAACGTCCTATATCATTAGGGATTTTCCCTCTACCTGCTGGAGAT GGATTGCTTACACCTGACACTCAGAAAGGAGGCGAGACCCCAGGATCAGAGCAATGGAAATTTCAAGAAT TAAGTCAACCACGTTCTCATACCAGCCTGAAGGATGAACTTTCTGATATTAGTCAAGGTGGATCTAAAGC TACCACTCCAGCTTCAACAGCAAATTCAGACGTATCAGCAATTCCTCCTGATACTCCGTCAAAGGAAGAT AATGAAGGATTTGTAAAAGGCACAGATACATCAAATAAGTCAGAGATAAGCAAACACATAGAAGTCCAGG TTGCCCAAGAGACTAGGAATGTGTCTACAGAATCTGGTGAAAATGAAGAGAAATCAGAAGTTCAAGCAAT CATTGAATCCACTCCTGAATTGGATATGGACAAAGACCTCAGTGGATATAAAGGTTCAAGCACTCCCACC AAAGGCATAGAGAACAAAGCTTTTGATCGCAATACAGAATCTCTCTTTGAAGAGCTGTCCTCAGCTGGCT CGGGCCTTATTGGAGATGTGGATGAAGGAGCGGATTTACTAGACCATAATTTCTTTGGAATGGGTCGTGA GGTGGAGAATCTCATACTGGAGAACACACAGCTGCTGGAAACCAAAAACGCTCTGAATGTAGTGAAGAAT GACTTAATAGCCAAAGTGGATGAGCTGACCTGTGAGAAGGACGTGCTGCAGGGGGAGCTGGAGGCTGTGA AGCAAGCCAAGCTGAAGCTGGAGGACAAGAACAGAGAGCTGGAGGAGGAGCTCAGGAAAGCTCGTGCAGA AGCTGAAGATGCAAGGCAAAAAGCAAAAGACGATGATGATAGTGACATTCCCACAGCCCAGAGGAAGAGG TTTACCAGAGTAGAAATGGCCCGAGTTCTCATGGAGAGAAACCAGTACAAAGAGAGGCTGATGGAGCTTC AAGAAGCTGTTCGATGGACGGAGATGATCCGGGCCTCACGAGAGAACCCAGCTATGCAAGAAAAGAAAAG GTCAAGCATCTGGCAATTTTTCAGCCGACTTTTTAGCTCTTCAAGTAATGCAACAAAGAAGCCTGAACCC CCTGTTAACCTGAAGTACAACGCCCCCACCTCTCACGTGACTCCCTCCGTGAAGAAAAGGAGCAGCACTT TATCTCAGCTCCCTGGGGACAAGTCCAAGGCATTTGATTTCCTCAGTGAGGAAACTGAGGCTAGTTTAGC CTCCCGCAGAGAGCAAAAGAGAGAGCAGTATCGGCAGGTGAAGGCTCACGTTCAGAAGGAAGATGGCCGA GTGCAAGCTTTCGGCTGGAGTCTGCCTCAGAAGTACAAACAGGTAGCCAATGGTCAAGGGGAAACTAAGA TGAAAAATTTACCTGTGCCTGTCTATCTCAGACCACTAGACGAAAAAGATGCGTCTATGAAGCTGTGGTG TGCTGTAGGAGTCAATTTATCTGGTGGCAAGACAAGAGATGGCGGGTCTGTTGTTGGAGCGAGTGTATTT TACAAGGATATTGCTGGTTTGGACACTGAAGGCAGTAAACAGCGGAGTGCGTCTCAGAGTAGTTTAGACA AGTTGGATCAAGAACTCAAGGAACAGCAGAAAGAATTTAAAAATCAAGAAGAGTTGTCCAGTCAGGTCTG GATCTGTACCAGCACCCACTCAACTACAAAGGTTATCATCATTGATGCTGTTCAGCCTGGCAACATCCTA GATAGTTTCACTGTTTGCAATTCTCATGTTCTGTGCATTGCCAGTGTCCCAGGAGCTCGGGAAACAGACT ACCCTGCAGGAGAAGAACTTTCTGAATCTGGTCAGGTAGACAAAGCGTCATTGTGTGGAAGCATGACCAG TAACAGCTCCGCAGAGATGGACAGCTTGCTGGGAGGCATCACAGTGGTTGGGTGCTCCACAGAAGGACTG ACAGGAGCTGCCACTTCCCCAAGTACCAACGGTGCTTCTCCTGTGATTGAAAAGCCACCAGAAATGGAAA CTGAAAATAGCGAAGTTGATGAAAATATTCCAACAGCAGAAGAAGCAACTGAAGCCACAGAGGGCAATGC AGGGTCCACTGAAGACACTGTGGACATCTCCCAGCCTGGCGTGTACACAGAGCATGTGTTTACAGATCCA CTGGGAGTTCAGATCCCAGAAGACCTCTCCCCAGTGTTTCAGTCAAGTAATGACTCAGATGTGTACAAAG ATCAAATATCAGTATTGCCAAATGAACAAGACCTTGCGAGAGAAGAAGCTCAGAAAATGAGTAGTCTTTT ACCAACCATGTGGCTTGGAGCTCAGAATGGCTGTTTGTATGTCCACTCATCTGTAGCCCAGTGGAGGAAA TGTCTCCATTCCATTAAGCTCAAAGACTCAATTCTCAGTATTGTACATGTGAAAGGAATTGTACTAGTGG CCCTGGCGGATGGCACCCTTGCAATCTTCCACAGAGGAGTTGATGGACAATGGGATCTGTCAAACTATCA CCTTTTAGACCTTGGACGTCCTCATCATTCCATACGATGCATGACTGTGGTACATGACAAAGTCTGGTGT GGCTATAGGAACAAAATCTATGTGGTTCAACCAAAGGCTATGAAGATAGAGAAATCATTTGATGCACACC CCAGGAAGGAGAGCCAAGTACGGCAGCTTGCATGGGTGGGTGATGGTGTATGGGTCTCCATTCGTTTGGA TTCCACGCTCCGCCTCTATCATGCACACACATACCAACATCTCCAGGATGTGGATATTGAGCCTTATGTA AGCAAAATGTTAGGTACTGGAAAACTGGGTTTCTCTTTCGTGAGGATAACAGCTCTTATGGTGTCTTGCA ATCGTTTGTGGGTGGGGACTGGAAATGGTGTCATTATCTCCATCCCACTGACAGAAACAAATAAAACCTC AGGGACTCCAGGTAACCGTCCTGGAAGTGTAATCCGTGTATATGGTGATGAGAACAGTGATAAAGTGACT CCAGGGACATTTATACCCTACTGTTCAATGGCACATGCACAGCTTTGTTTCCACGGGCACCGGGATGCTG TGAAATTCTTTGTGGCAGTCCCAGGTCAGGTCATTAGCCCACAAAGTAGCAGTGGTGGGGCAGATCTTAC AGCTGACAAGGCAGGGTCATCTGCACAGGAGCCCAGTAGCCAGACGCCCTTGAAGTCCATGCTTGTCATC AGTGGAGGAGAGGGCTACATTGACTTCCGGATGGGTGATGAAGGTGGAGAATCTGAACTTCTGGGAGAGG ATCTTCCACTGGAACCTTCAGTCACCAAAGCCGAGAGGAGTCACCTGATAGTGTGGCAAGTGATGTGTGG CAATGAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG221375 representing NM_001025428
Red=Cloning site Green=Tags(s) MNPGCMLLFVFGFVGGAVVINSAILVSLSVLLLVHFSISTGVPALTQNLPRILRKERPISLGIFPLPAGD GLLTPDTQKGGETPGSEQWKFQELSQPRSHTSLKDELSDISQGGSKATTPASTANSDVSAIPPDTPSKED NEGFVKGTDTSNKSEISKHIEVQVAQETRNVSTESGENEEKSEVQAIIESTPELDMDKDLSGYKGSSTPT KGIENKAFDRNTESLFEELSSAGSGLIGDVDEGADLLDHNFFGMGREVENLILENTQLLETKNALNVVKN DLIAKVDELTCEKDVLQGELEAVKQAKLKLEDKNRELEEELRKARAEAEDARQKAKDDDDSDIPTAQRKR FTRVEMARVLMERNQYKERLMELQEAVRWTEMIRASRENPAMQEKKRSSIWQFFSRLFSSSSNATKKPEP PVNLKYNAPTSHVTPSVKKRSSTLSQLPGDKSKAFDFLSEETEASLASRREQKREQYRQVKAHVQKEDGR VQAFGWSLPQKYKQVANGQGETKMKNLPVPVYLRPLDEKDASMKLWCAVGVNLSGGKTRDGGSVVGASVF YKDIAGLDTEGSKQRSASQSSLDKLDQELKEQQKEFKNQEELSSQVWICTSTHSTTKVIIIDAVQPGNIL DSFTVCNSHVLCIASVPGARETDYPAGEELSESGQVDKASLCGSMTSNSSAEMDSLLGGITVVGCSTEGL TGAATSPSTNGASPVIEKPPEMETENSEVDENIPTAEEATEATEGNAGSTEDTVDISQPGVYTEHVFTDP LGVQIPEDLSPVFQSSNDSDVYKDQISVLPNEQDLAREEAQKMSSLLPTMWLGAQNGCLYVHSSVAQWRK CLHSIKLKDSILSIVHVKGIVLVALADGTLAIFHRGVDGQWDLSNYHLLDLGRPHHSIRCMTVVHDKVWC GYRNKIYVVQPKAMKIEKSFDAHPRKESQVRQLAWVGDGVWVSIRLDSTLRLYHAHTYQHLQDVDIEPYV SKMLGTGKLGFSFVRITALMVSCNRLWVGTGNGVIISIPLTETNKTSGTPGNRPGSVIRVYGDENSDKVT PGTFIPYCSMAHAQLCFHGHRDAVKFFVAVPGQVISPQSSSGGADLTADKAGSSAQEPSSQTPLKSMLVI SGGEGYIDFRMGDEGGESELLGEDLPLEPSVTKAERSHLIVWQVMCGNE TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001025428 |
| ORF Size | 3507 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001025428.2 |
| RefSeq Size | 6931 bp |
| RefSeq ORF | 3510 bp |
| Locus ID | 70834 |
| UniProt ID | Q58A65 |
| Gene Summary | The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module (PubMed:12391307, PubMed:15767678). Regulates lysosomal positioning by acting as an adapter protein which links PIP4P1-positive lysosomes to the dynein-dynactin complex (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR221375 | Spag9 (Myc-DDK-tagged) - Mouse sperm associated antigen 9 (Spag9), transcript variant 2 |
CNY 11,690.00 |
|
| MR221375L3 | Lenti ORF clone of Spag9 (Myc-DDK-tagged) - Mouse sperm associated antigen 9 (Spag9), transcript variant 2 |
CNY 13,590.00 |
|
| MR221375L4 | Lenti ORF clone of Spag9 (mGFP-tagged) - Mouse sperm associated antigen 9 (Spag9), transcript variant 2 |
CNY 13,590.00 |
