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Mid1 (NM_183151) Mouse Tagged ORF Clone
CAT＃: MG220976
- TrueORF®
Mid1 (tGFP-tagged) - Mouse midline 1 (Mid1) transcript variant 2, (10ug)
ORF Plasmid: DDK
"NM_183151" in other vectors (4)
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CNY 9,072.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 61B3-R; DXHXS1141; Fxy; Trim18 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG220976 representing NM_183151
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAACACTGGAGTCGGAGCTGACCTGTCCTATTTGTCTGGAGCTTTTTGAGGACCCTCTCCTGCTTC CCTGCGCACATAGCCTCTGCTTCAACTGTGCCCACCGAATCCTGGTCTCTCACTGTGCCACCAACGAGCC TGTGGAGTCCATCAACGCCTTCCAGTGCCCCACCTGCCGGCATGTCATCACGCTCAGCCAGCGAGGTCTA GACGGGCTCAAGCGCAACGTCACCCTCCAAAACATCATTGACAGATTTCAGAAAGCATCTGTGAGCGGGC CCAACTCTCCCAGTGAGACCCGCAGGGAACGGGCCTTTGACGCCAACACCATGTCCTCTGCCGAGAAGGT TCTCTGCCAGTTCTGTGACCAGGATCCTGCCCAGGATGCTGTGAAGACTTGCGTTACTTGTGAAGTGTCC TACTGTGATGAGTGCCTGAAAGCCACTCATCCGAACAAGAAGCCCTTTACAGGCCATCGTCTGATTGAGC CAATCCCGGACTCGCACATCCGGGGGCTGATGTGCCTGGAGCACGAGGATGAGAAGGTGAATATGTACTG TGTGACCGATGACCAGTTAATCTGTGCCTTGTGTAAACTGGTTGGGCGGCACCGCGATCATCAGGTGGCA GCTTTGAGTGAGCGCTATGACAAATTGAAGCAAAACTTGGAGAGTAATCTCACCAATCTTATTAAGAGAA ACACAGAACTGGAGACTCTTTTGGCTAAACTCATCCAAACTTGTCAACATGTTGAAGTCAATGCATCCCG TCAAGAAGCCAAACTGACAGAAGAATGTGATCTTCTCATTGAAATCATTCAGCAACGAAGACAAATTATT GGAACAAAGATTAAAGAAGGCAAGGTGATCAGGCTCCGCAAGTTAGCTCAGCAGATTGCAAACTGTAAAC AGTGCCTTGAGAGGTCTGCATCGCTCATCTCGCAAGCGGAGCACTCGCTGAAGGAAAATGACCACGCCCG TTTTCTACAGACAGCAAAGAATATCACTGAGAGAGTCTCCATGGCAACTGCATCCTCCCAGGTCCTAATT CCCGAAATCAACCTCAATGACACGTTTGACACTTTTGCCTTGGATTTTTCCCGGGAGAAGAAACTGCTAG AATGTCTGGATTACCTAACAGCTCCCAACCCTCCCGCGATTAGAGAAGAGCTCTGCACCGCTTCCTACGA CACCATCACCGTCCACTGGACCTCAGAGGACGAGTTCAGCGTGGTCTCCTACGAGCTCCAGTACACCATA TTCACCGGACAAGCCAATGTTGTCAACGTGGCCTGTGACGGCACCTGTCTCCTCGGCTCCGCAGGTCTGT GTAACTCGGCGGACAGCTGGATGATCGTGCCCAACATCAAGCAGAACCACTACACCGTGCACGGCCTGCA AAGTGGCACCAAGTATATCTTCACGGTGAAGGCCATCAACCAGGCGGGCAGCCGTAGCAGCGAGCCCGGA AAGCTGAAGACCAACAGTCAGCCGTTTAGACTGGATCCCAAATCGGCTCATCGCAAGCTGAAGGTGTCCC ACGACAACCTGACTGTCGAGCGCGACGAGTCGTCCTCTAAGAAGAGTCACGCGCCGGAGCGCTTCGCTGG TCAGGGGAGCTACGGAGTGGCTGGCAACGTGTTCATCGACAGCGGCCGTCACTACTGGGAAGTGGTCACC AGCGGAAGCACATGGTACGCCATCGGCCTGGCGTACAGATCGGCGCCGAAACACGAGTGGATCGGGAAGA ACGCGGCGTCCTGGGCCCTCTGCCGCTGCCACAACCACTGGGCGGTGCGACACGACGGCAAGGAGACCCC CATCGCGCCGGCCCCTCACCTCAGGCGCGTCGGCGTCCTGCTGGACTACGACAACGGCTCCATCGCCTTC TACGACGCTCTGAGCTCCGTCCACCTCCACACCTTCCACGCGGCGCTCGCGCAGCCCGTGTGCCCCACCT TCACCGTGTGGAACAAGTGTCTGACCATCGTCACGGGTCTGCCCATCCCGGACCATCTGGACTGTACGGA GCAGCGACCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG220976 representing NM_183151
Red=Cloning site Green=Tags(s) METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNEPVESINAFQCPTCRHVITLSQRGL DGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMSSAEKVLCQFCDQDPAQDAVKTCVTCEVS YCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVA ALSERYDKLKQNLESNLTNLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLTEECDLLIEIIQQRRQII GTKIKEGKVIRLRKLAQQIANCKQCLERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLI PEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPAIREELCTASYDTITVHWTSEDEFSVVSYELQYTI FTGQANVVNVACDGTCLLGSAGLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFTVKAINQAGSRSSEPG KLKTNSQPFRLDPKSAHRKLKVSHDNLTVERDESSSKKSHAPERFAGQGSYGVAGNVFIDSGRHYWEVVT SGSTWYAIGLAYRSAPKHEWIGKNAASWALCRCHNHWAVRHDGKETPIAPAPHLRRVGVLLDYDNGSIAF YDALSSVHLHTFHAALAQPVCPTFTVWNKCLTIVTGLPIPDHLDCTEQRP TRTRPLE - GFP Tag - V |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_183151 |
| ORF Size | 2040 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_183151.1, NP_898974.1 |
| RefSeq Size | 3974 bp |
| RefSeq ORF | 2043 bp |
| Locus ID | 17318 |
| Gene Summary | Has E3 ubiquitin ligase activity towards IGBP1, promoting its monoubiquitination, which results in deprotection of the catalytic subunit of protein phosphatase PP2A, and its subsequent degradation by polyubiquitination.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220411 | Mid1 (untagged) - Mouse midline 1 (Mid1), transcript variant 2, (10ug) |
CNY 8,740.00 |
|
| MR220976 | Mid1 (Myc-DDK-tagged) - Mouse midline 1 (Mid1), transcript variant 2 |
CNY 7,472.00 |
|
| MR220976L3 | Lenti ORF clone of Mid1 (Myc-DDK-tagged) - Mouse midline 1 (Mid1), transcript variant 2 |
CNY 8,650.00 |
|
| MR220976L4 | Lenti ORF clone of Mid1 (mGFP-tagged) - Mouse midline 1 (Mid1), transcript variant 2 |
CNY 8,650.00 |
