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Rad51b (NM_009014) Mouse Tagged ORF Clone
CAT＃: MG220772
- TrueORF®
Rad51b (tGFP-tagged) - Mouse RAD51-like 1 (S. cerevisiae) (Rad51l1), (10ug)
ORF Plasmid: DDK
"NM_009014" in other vectors (4)
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CNY 3,140.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AI553500; mREC2; R51H2; Rad51l1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG220772 representing NM_009014
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCAGCAAGAAACTAAGACGAGTGGGTTTATCTCCAGAGCTGTGTGACCGTTTAAGCAGATACCAGA TTGTTAACTGTCAGCACTTTTTAAGTCTCTCCCCACTAGAACTTATGAAAGTGACTGGCCTGAGTTACAG AGGTGTCCACGAGCTTCTTCATACAGTAAGCAAGGCCTGTGCCCCGCAGATGCAAACGGCTTATGAGTTA AAGACACGAAGGTCTGCACATCTCTCACCGGCATTCCTGTCTACTACCCTGTGCGCCTTGGATGAAGCAT TGCACGGTGGTGTGCCTTGTGGATCTCTCACAGAGATTACAGGTCCACCAGGTTGCGGAAAAACTCAGTT TTGCATAATGATGAGTGTCTTAGCTACATTACCTACCAGCCTGGGAGGATTAGAAGGGGCTGTGGTCTAC ATCGACACAGAGTCTGCATTTACTGCTGAGAGACTGGTTGAGATTGCGGAATCTCGTTTTCCACAATATT TTAACACTGAGGAAAAATTGCTTCTGACCAGCAGTAGAGTTCATCTTTGCCGAGAGCTCACCTGTGAGGG GCTTCTACAAAGGCTTGAGTCTTTGGAGGAAGAGATCATTTCGAAAGGAGTTAAGCTTGTGATTGTTGAC TCCATTGCTTCTGTGGTCAGAAAGGAGTTTGACCCGAAGCTTCAAGGCAACATCAAAGAAAGGAACAAGT TCTTGGGCAAAGGAGCGTCCTTACTGAAGTACCTGGCAGGGGAGTTTTCAATCCCAGTTATCTTGACGAA TCAAATTACGACCCATCTGAGTGGAGCCCTCCCTTCTCAAGCAGACCTGGTGTCTCCAGCTGATGATTTG TCCCTGTCTGAAGGCACTTCTGGATCCAGCTGTTTGGTAGCTGCACTAGGAAACACATGGGGTCACTGTG TGAACACCCGGCTGATTCTCCAGTACCTTGATTCAGAGAGAAGGCAGATTCTCATTGCCAAGTCTCCTCT GGCTGCCTTCACCTCCTTTGTCTACACCATCAAGGGGGAAGGCCTGGTTCTTCAAGGCCACGAAAGACCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG220772 representing NM_009014
Red=Cloning site Green=Tags(s) MSSKKLRRVGLSPELCDRLSRYQIVNCQHFLSLSPLELMKVTGLSYRGVHELLHTVSKACAPQMQTAYEL KTRRSAHLSPAFLSTTLCALDEALHGGVPCGSLTEITGPPGCGKTQFCIMMSVLATLPTSLGGLEGAVVY IDTESAFTAERLVEIAESRFPQYFNTEEKLLLTSSRVHLCRELTCEGLLQRLESLEEEIISKGVKLVIVD SIASVVRKEFDPKLQGNIKERNKFLGKGASLLKYLAGEFSIPVILTNQITTHLSGALPSQADLVSPADDL SLSEGTSGSSCLVAALGNTWGHCVNTRLILQYLDSERRQILIAKSPLAAFTSFVYTIKGEGLVLQGHERP TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009014 |
| ORF Size | 1050 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009014.3, NP_033040.2 |
| RefSeq Size | 1858 bp |
| RefSeq ORF | 1053 bp |
| Locus ID | 19363 |
| UniProt ID | O35719 |
| Gene Summary | Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. May promote the assembly of presynaptic RAD51 nucleoprotein filaments. Binds single-stranded DNA and double-stranded DNA and has DNA-dependent ATPase activity. Part of the RAD21 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. BCDX2 binds predominantly to the intersection of the four duplex arms of the Holliday junction and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. The BCDX2 subcomplex RAD51B:RAD51C exhibits single-stranded DNA-dependent ATPase activity suggesting an involvement in early stages of the HR pathway (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC209283 | Rad51b (untagged) - Mouse RAD51-like 1 (S. cerevisiae) (Rad51l1), (10ug) |
CNY 3,990.00 |
|
| MR220772 | Rad51b (Myc-DDK-tagged) - Mouse RAD51-like 1 (S. cerevisiae) (Rad51l1) |
CNY 2,850.00 |
|
| MR220772L3 | Lenti ORF clone of Rad51b (Myc-DDK-tagged) - Mouse RAD51-like 1 (S. cerevisiae) (Rad51l1) |
CNY 4,750.00 |
|
| MR220772L4 | Lenti ORF clone of Rad51b (mGFP-tagged) - Mouse RAD51-like 1 (S. cerevisiae) (Rad51l1) |
CNY 4,750.00 |
