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Xylt1 (NM_175645) Mouse Tagged ORF Clone
CAT＃: MG218127
- TrueORF®
Xylt1 (tGFP-tagged) - Mouse xylosyltransferase 1 (Xylt1), (10ug)
ORF Plasmid: DDK
"NM_175645" in other vectors (4)
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CNY 7,890.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 8030490L12 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG218127 representing NM_175645
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGGCGGCGCCGTGCGCCCGGAGGCTGGCGCGGCGCTCGCACTCGGCGCTGCTGGCGGCGCTCATGG TGCTGCTGCTGCAGACGCTGGTGGTGTGGAATTTCAGCAGCCTGGACTCCGGGGCTGGAGAGCAGCGGCG CGCGGGGGCAGCGGCGGGAGCAGCCGAGCAGCAGCAGCCCGCGGCCCCGCGCCGGGAGCGCAGGGACCTG GCTGCCCATCTGCCCGCAGCCCGCGGAGGACCTGGAGGCCGAGCCGGCGGAGGAGGAGCACGGGGAGGCG GCCCCGGAGGAGCCCGCGCACAGCAGCCTGCCAGCCGCGGGGCACTAGCCTCCCGGGCGAGGGATCCACA ACCAAGTCCACTGATCACCCTAGAGACCCAGGATGGCTACTTCTCTCACCGTCCCAAAGAGAAAGTTCGG ACCGACAGCAACAATGAGAACTCAGTCCCCAAGGACTTTGAGAATGTGGATAACAGCAACTTCGCCCCCA GGACCCAGAAGCAGAAACACCAGCCAGAGCTGGCAAAGAAGCCCCCCAGCAGGCAGAAAGAGCATTTGCA GAGAAAGCTGGATGCCCAGGACAAACGACAGGGCCAGTCAGTCCTGGGGAAAGGCCCCAAGGAGGTGCTG CCTCCTCGGGAGAAAGCCACAGGCAACAGTAGCCAAGGGAAGGATCTCTCAAGACACAGCCATGCCAGGA AGAGTGGTGGTGGTGGGTCCCCCGAAACCAAGTCTGACCAGGCCCCCAAGTGTGATATCTCTGGCAAGGA GGCCATCTCAGCACTGACCCGCGCTAAGTCCAAGCATTGTCGCCAGGAGATTGCAGAAACCTACTGTCGC CACAAGCTGGGGCTGCTGATGCCAGAGAAGGTGGCTCGATTCTGTCCCCTAGAAGGCAAAGCCAACAAGA ATGTCCAGTGGGATGAGGATGCTGTGGAGTACATGCCTGCCAACCCAGTCAGAATCGCCTTTGTCCTGGT GGTCCATGGCCGTGCCTCTCGACAGCTACAGCGCATGTTCAAGGCCATCTACCACAAAGACCATTTCTAC TATATCCATGTGGATAAGCGTTCCAATTACCTGCATCGGCAAGTGCTCCAGTTCTCCAGGCAGTACGACA ATGTCCGAGTCACCTCCTGGAGGATGGCCACCATTTGGGGTGGAGCCAGCCTCCTGTCCACCTACCTGCA GAGCATGCGGGATCTACTAGAGATGACTGACTGGCCCTGGGACTTCTTCATCAACCTCAGTGCTGCTGAC TACCCCATCAGGACAAATGACCAGCTGGTAGCATTTCTTTCCAGATATCGAGATATGAACTTCCTGAAGT CACATGGCCGGGACAATGCAAGGTTCATCCGGAAGCAGGGCCTGGACCGCCTCTTCCTGGAGTGTGATAC ACACATGTGGCGCCTGGGGGACCGGCGGATCCCAGAGGGCATTGCTGTGGATGGTGGTTCTGATTGGTTC CTGCTAAACAGGAAGTTTGTAGAGTATGTGGCATTCTCCACAGATGACCTGGTGACCAAGATGAAGCAGT TCTACTCTTACACCCTTCTCCCTGCTGAGTCCTTTTTCCACACGGTTCTAGAGAACAGCCCCCACTGTGA CACCATGGTGGATAACAACCTGCGCATCACCAACTGGAACCGCAAGCTGGGCTGCAAGTGCCAGTACAAG CATATCGTGGACTGGTGCGGCTGCTCTCCCAATGACTTCAAGCCTCAGGACTTCCATCGCTTCCAGCAGA CAGCCCGGCCCACCTTCTTTGCCCGAAAGTTCGAAGCCATAGTGAACCAGGAGATCATTGGGCAGCTGGA CTCTTACCTGTACGGGAACTATCCTGCGGGCACCCCGGGCCTCCGCTCCTACTGGGAGAATGTCTACGAT GAACCAGATGGCATCCACACCCTCAGCGATGTAGCCCTCACCCTGTACCATTCCTTCATCCGTCTGGGTC TTCGAAGAGCTGAGTCATCCCTACACACGGATGGGGAGAACAGCTGCAGGTACTACCCAATGGGCCACCC AGCTTCTGTCCATCTCTACTTCCTTGCTGACCGATTCCAGGGCTTTCTGATCAAGCATCACGTGACCAAC CTTGCTGTGAGCAAACTGGAGACACTGGAGACATGGATGATGCCAAAGAAAGTCTTCAAGGTCGCAAGTC CCCCCAGTGACTTTGGAAGGCTTCAGTTTTCTGAGGTTGGCACTGACTGGGATGCCAAGGAGAGGCTGTT CCGGAACTTTGGTGGTCTTTTGGGGCCCATGGATGAGCCGGTGGGGATGCAGAAATGGGGAAAGGGGCCC AATGTGACCGTGACTGTTATTTGGGTGGATCCTGTCAACGTCATTGCAGCCACCTATGATATCCTGATTG AGTCCACTGCGGAATTCACACACTACAAGCCCCCTTTGAATCTGCCTCTGAGGCCTGGGGTCTGGACAGT GAAGATTCTCCATCACTGGGTGCCAGTGGCAGAGACCAAATTCCTTGTGGCACCTTTGACCTTTTCCAAC AAGCAGCCCATCAAACCAGAGGAGGCTTTGAAGCTGCACAATGGGCCACCTCGCAGTGCCTACATGGAGC AGAGTTTCCAGAGCCTGAACCCAGTCCTCAGCCTGCACATCAATCCTGCCCAAGTCGAACAGGCCCGGAA GAATGCAGCCTTCACCGGGACAGCGCTAGAAGCCTGGCTGGACTCGCTGGTGGGTGGGACTTGGACTGCC ATGGACATCTGCACCACAGGCCCCACCGCCTGCCCAGTCATGCAGACCTGCAGCCAAACAGCCTGGAGTT CCTTCAGCCCTGATCCCAAGTCAGAGCTGGGTGCAGTCAAACCTGACGGACGGCTCAGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG218127 representing NM_175645
Red=Cloning site Green=Tags(s) MVAAPCARRLARRSHSALLAALMVLLLQTLVVWNFSSLDSGAGEQRRAGAAAGAAEQQQPAAPRRERRDL AAHLPAARGGPGGRAGGGGARGGGPGGARAQQPASRGALASRARDPQPSPLITLETQDGYFSHRPKEKVR TDSNNENSVPKDFENVDNSNFAPRTQKQKHQPELAKKPPSRQKEHLQRKLDAQDKRQGQSVLGKGPKEVL PPREKATGNSSQGKDLSRHSHARKSGGGGSPETKSDQAPKCDISGKEAISALTRAKSKHCRQEIAETYCR HKLGLLMPEKVARFCPLEGKANKNVQWDEDAVEYMPANPVRIAFVLVVHGRASRQLQRMFKAIYHKDHFY YIHVDKRSNYLHRQVLQFSRQYDNVRVTSWRMATIWGGASLLSTYLQSMRDLLEMTDWPWDFFINLSAAD YPIRTNDQLVAFLSRYRDMNFLKSHGRDNARFIRKQGLDRLFLECDTHMWRLGDRRIPEGIAVDGGSDWF LLNRKFVEYVAFSTDDLVTKMKQFYSYTLLPAESFFHTVLENSPHCDTMVDNNLRITNWNRKLGCKCQYK HIVDWCGCSPNDFKPQDFHRFQQTARPTFFARKFEAIVNQEIIGQLDSYLYGNYPAGTPGLRSYWENVYD EPDGIHTLSDVALTLYHSFIRLGLRRAESSLHTDGENSCRYYPMGHPASVHLYFLADRFQGFLIKHHVTN LAVSKLETLETWMMPKKVFKVASPPSDFGRLQFSEVGTDWDAKERLFRNFGGLLGPMDEPVGMQKWGKGP NVTVTVIWVDPVNVIAATYDILIESTAEFTHYKPPLNLPLRPGVWTVKILHHWVPVAETKFLVAPLTFSN KQPIKPEEALKLHNGPPRSAYMEQSFQSLNPVLSLHINPAQVEQARKNAAFTGTALEAWLDSLVGGTWTA MDICTTGPTACPVMQTCSQTAWSSFSPDPKSELGAVKPDGRLR TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_175645 |
| ORF Size | 2859 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_175645.3, NP_783576.2 |
| RefSeq Size | 3070 bp |
| RefSeq ORF | 2862 bp |
| Locus ID | 233781 |
| UniProt ID | Q811B1 |
| Gene Summary | Catalyzes the first step in the biosynthesis of chondroitin sulfate and dermatan sulfate proteoglycans, such as DCN. Transfers D-xylose from UDP-D-xylose to specific serine residues of the core protein. Required for normal maturation of chondrocytes during bone development, normal onset of ossification and normal embryonic and postnatal skeleton development, especially of the long bones.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222809 | Xylt1 (untagged) - Mouse xylosyltransferase 1 (Xylt1), (10ug) |
CNY 12,160.00 |
|
| MR218127 | Xylt1 (Myc-DDK-tagged) - Mouse xylosyltransferase 1 (Xylt1) |
CNY 6,976.00 |
|
| MR218127L3 | Lenti ORF clone of Xylt1 (Myc-DDK-tagged) - Mouse xylosyltransferase 1 (Xylt1) |
CNY 9,030.00 |
|
| MR218127L4 | Lenti ORF clone of Xylt1 (mGFP-tagged) - Mouse xylosyltransferase 1 (Xylt1) |
CNY 9,030.00 |
