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Ensa (NM_019561) Mouse Tagged ORF Clone
CAT＃: MG217826
- TrueORF®
Ensa (tGFP-tagged) - Mouse endosulfine alpha (Ensa) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_019561" in other vectors (4)
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CNY 2,090.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 1700020C18Rik; 2610007F17Rik; AI451924 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG217826 representing NM_019561
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCCAGAAACAAGAAGAAGAAAACCCTGCGGAGGAGACCGGCGAGGAGAAGCAGGATACACAGGAGA AAGAAGGGATTCTCCCTGAGAAAGCTGAGGAGGCAAAGCTAAAGGCCAAATATCCAAGCCTAGGACAAAA GCCTGGAGGCTCCGACTTCCTCATGAAGAGACTCCAGAAAGGGCAAAAGTACTTTGACTCAGGAGACTAC AACATGGCCAAAGCCAAGATGAAGAACAAGCAGCTGCCAAGTGCAGGAGCAGACAAGAACCTGGTGACCG GTGACCACATCCCCACCCCACAGGACCTGCCCCAGAGAAAGTCCTCGCTCGTCACCAGCAAGCTTGCGGG TGGCCAAGTTGAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG217826 representing NM_019561
Red=Cloning site Green=Tags(s) MSQKQEEENPAEETGEEKQDTQEKEGILPEKAEEAKLKAKYPSLGQKPGGSDFLMKRLQKGQKYFDSGDY NMAKAKMKNKQLPSAGADKNLVTGDHIPTPQDLPQRKSSLVTSKLAGGQVE TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_019561 |
| ORF Size | 363 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_019561.2, NP_062507.1 |
| RefSeq Size | 1192 bp |
| RefSeq ORF | 366 bp |
| Locus ID | 56205 |
| UniProt ID | P60840 |
| Gene Summary | Protein phosphatase inhibitor that specifically inhibits protein phosphatase 2A (PP2A) during mitosis. When phosphorylated at Ser-67 during mitosis, specifically interacts with PPP2R2D (PR55-delta) and inhibits its activity, leading to inactivation of PP2A, an essential condition to keep cyclin-B1-CDK1 activity high during M phase. Also acts as a stimulator of insulin secretion by interacting with sulfonylurea receptor (ABCC8), thereby preventing sulfonylurea from binding to its receptor and reducing K(ATP) channel currents (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC210028 | Ensa (untagged) - Mouse endosulfine alpha (Ensa), transcript variant 1, (10ug) |
CNY 3,990.00 |
|
| MR217826 | Ensa (Myc-DDK-tagged) - Mouse endosulfine alpha (Ensa), transcript variant 1 |
CNY 1,900.00 |
|
| MR217826L3 | Lenti ORF clone of Ensa (Myc-DDK-tagged) - Mouse endosulfine alpha (Ensa), transcript variant 1 |
CNY 3,800.00 |
|
| MR217826L4 | Lenti ORF clone of Ensa (mGFP-tagged) - Mouse endosulfine alpha (Ensa), transcript variant 1 |
CNY 3,800.00 |
