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Ank3 (NM_170687) Mouse Tagged ORF Clone
CAT＃: MG215427
- TrueORF®
Ank3 (tGFP-tagged) - Mouse ankyrin 3 epithelial (Ank3) transcript variant 6, (10ug)
ORF Plasmid: DDK
"NM_170687" in other vectors (4)
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CNY 12,070.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2900054D09Rik; AI314020; An; Ank; Ank-3; AnkG; Anky; Ankyrin-3; Ankyrin-G |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG215427 representing NM_170687
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTTGCCACACAGTGAAGATGCCATCACAGGGGACACTGACAAGTATCTCGGGCCACAGGACCTTA AGGAGCTAGGTGATGACTCCCTGCCAGCAGAAGGTTACGTAGGCTTCAGTCTTGGAGCCCGTTCTGCCAG CCTCCGCTCCTTCAGTTCGGATAGGTCCTACACCTTGAACAGAAGCTCCTACGCAAGGGACAGCATGATG ATAGAGGAACTTCTGGTACCATCCAAAGAGCAGCACCTGACGTTCACGAGGGAGTTTGATTCTGACTCCC TCAGACACTACAGTTGGGCAGCGGACACGTTAGATAATGTGAACCTGGTCTCAAGCCCGGTGCATTCTGG GTTTCTGGTTAGCTTTATGGTGGACGCGAGAGGGGGCTCCATGCGAGGAAGCCGCCACCACGGGATGCGG ATCATCATCCCTCCGCGAAAGTGTACGGCCCCCACCCGCATCACGTGCCGCCTGGTAAAGAGACATAAAC TGGCCAACCCACCCCCCATGGTGGAAGGAGAGGGATTAGCCAGTAGGCTGGTAGAAATGGGTCCTGCGGG GGCACAATTTTTAGGCCCCGTCATTGTGGAAATCCCTCATTTTGGGTCCATGAGGGGGAAGGAGAGAGAA CTTATCGTCCTTCGGAGCGAGAACGGAGAGACCTGGAAGGAACATCAGTTTGACAGTAAAAACGAAGACC TCGCGGAGCTTCTCAATGGCATGGATGAAGAACTCGACAGCCCGGAAGAGTTGGGTACAAAGCGCATCTG CAGAATTATCACAAAGGATTTCCCCCAGTATTTTGCCGTGGTTTCCCGGATTAAGCAGGAAAGCAACCAG ATCGGTCCTGAGGGTGGGATTCTGAGCAGCACCACCGTGCCCCTCGTCCAGGCCTCCTTCCCAGAGGGCG CCTTAACCAAGAGGATCCGTGTGGGTCTCCAGGCTCAGCCCGTGCCAGAGGAAACGGTAAAAAAAATCCT TGGGAACAAAGCAACATTTAGCCCAATTGTCACGGTAGAGCCGAGGAGAAGGAAGTTCCATAAGCCGATC ACCATGACCATTCCGGTGCCCCCGCCCTCGGGAGAAGGCGTGTCCAATGGGTACAAGGGGGATGCCACGC CCAACCTGCGGCTCCTCTGCAGCATCACAGGAGGCACCTCACCAGCTCAATGGGAAGACATCACAGGAAC AACCCCTCTGACGTTCATAAAGGATTGTGTGTCTTTCACAACCAACGTTTCAGCCAGATTCTGGCTGGCG GACTGCCATCAGGTGTTAGAGACCGTAGGGCTAGCCTCCCAGCTGTACAGAGAGCTGATATGCGTTCCCT ACATGGCCAAGTTCGTTGTGTTTGCCAAAACAAACGACCCGGTGGAGTCCTCGCTGAGGTGCTTCTGTAT GACAGACGACAGGGTGGACAAAACCCTGGAGCAGCAGGAGAACTTCGAGGAGGTTGCCAGAAGCAAAGAC ATTGAGGTTCTGGAAGGAAAGCCCATCTACGTTGATTGCTATGGAAACCTGGCCCCTCTGACCAAAGGAG GACAGCAGCTTGTTTTTAACTTTTATTCTTTCAAAGAAAACAGACTGCCATTTTCCATCAAGATCAGAGA CACCAGTCAAGAGCCCTGTGGCCGCCTGTCTTTCCTGAAGGAGCCAAAGACAACAAAGGGATTACCCCAA ACAGCTGTTTGCAACTTAAATATTACTCTGCCGGCACATAAAAAGGCTGAGAAGGCAGACAGACGCCAGA GCTTTGCCTCCCTAGCTTTACGTAAGCGCTACAGCTACTTGACTGAACCCAGCATGAGTCCGCAGAGTCC TTGTGAGCGGACGGATATCAGGATGGCGATAGTAGCCGATCACCTGGGACTTAGTTGGACAGAGCTGGCA AGGGAACTGAATTTTTCAGTGGATGAAATCAACCAAATACGTGTGGAAAATCCCAATTCTTTAATTTCTC AGAGCTTCATGTTATTAAAAAAGTGGGTGACCAGAGACGGAAAGAATGCCACAACTGATGCCTTAACTTC GGTCTTAACGAAGATTAACCGGATAGACATTGTAACTCTGCTGGAAGGACCAATATTTGATTATGGGAAT ATTTCAGGCACCAGAAGCTTTGCAGATGAAAACAATGTTTTCCATGACCCAGTTGATGGTCACCCTTCCT TTCAAGTGGAGCTGGAGACCCCCATGGGGTTGTACTGCACACCACCCAACCCTTTCCAGCAAGATGACCA TTTTAGTGATATCTCTAGCATAGAGTCTCCCTTTAGGACCCCCAGTAGACTGAGTGACGGGCTGGTGCCT TCCCAGGGAAACATAGAGCATCCAACAGGTGGACCTCCAGTGGTAACCGCAGAGGACACTTCTTTAGAAG ACAGCAAAATGGACGATTCTGTAACTGTAACAGACCCGGCCGACCCACTGGACGTAGATGAGAGCCAGTT GAAGGACCTGTGTCAGAGCGAGTGTGCTCAGTGCTGGGCGAGTGTGCCCGGGATCCCAAACGACGGTCGG CAGGCAGAGCCACTGAGACCGCAGACTAGAAAAGTAGGCATGAGCTCTGAACAGCAGGAAAAAGGAAAAT CTGGTCCTGATGAGGAAGTGACAGAAGACAAGGTCAAATCTCTGTTTGAGGACATTCAACTTGAAGAAGT AGAGGCTGAGGAGATGACAGAAGACCAGGGGCAGGCTATGCTTAACCGTGTTCAGCGAGCAGAACTGGCA ATGTCTTCACTTGCAGGTTGGCAGAACGAGACGCCAAGTGGAAGCCTAGAGTCCCCAGCGCAAGCTCGAA GACTAACTGGTGGGTTACTGGACCGTCTGGATGACAGCTCTGACCAGGCTCGGGATTCTATTACCTCATA CCTCACGGGAGAACCTGGGAAGATCGAAGCAAATGGAAACCACACAGCGGAAGTCATTCCAGAAGCAAAG GCAAAACCCTACTTCCCGGAATCCCAAAACGATATAGGGAAACAGAGCATCAAGGAGAACCTGAAACCAA AAACACACGGATGTGGTCGCACTGAGGAACCAGTGTCGCCCCTCACAGCCTACCAGAAATCTCTGGAAGA AACCAGCAAGCTTGTCATAGAAGACGCACCTAAACCCTGTGTGCCTGTCGGCATGAAAAAGATGACCAGG ACTACGGCTGACGGCAAAGCCAGGCTCAACCTCCAGGAAGAAGAGGGGTCCACCAGGTCAGAGCCTAAGC AGGGAGAAGGCTATAAGGTGAAGACGAAGAAGGAAATCCGGAACGTGGAGAAGAAAACCCAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG215427 representing NM_170687
Red=Cloning site Green=Tags(s) MALPHSEDAITGDTDKYLGPQDLKELGDDSLPAEGYVGFSLGARSASLRSFSSDRSYTLNRSSYARDSMM IEELLVPSKEQHLTFTREFDSDSLRHYSWAADTLDNVNLVSSPVHSGFLVSFMVDARGGSMRGSRHHGMR IIIPPRKCTAPTRITCRLVKRHKLANPPPMVEGEGLASRLVEMGPAGAQFLGPVIVEIPHFGSMRGKERE LIVLRSENGETWKEHQFDSKNEDLAELLNGMDEELDSPEELGTKRICRIITKDFPQYFAVVSRIKQESNQ IGPEGGILSSTTVPLVQASFPEGALTKRIRVGLQAQPVPEETVKKILGNKATFSPIVTVEPRRRKFHKPI TMTIPVPPPSGEGVSNGYKGDATPNLRLLCSITGGTSPAQWEDITGTTPLTFIKDCVSFTTNVSARFWLA DCHQVLETVGLASQLYRELICVPYMAKFVVFAKTNDPVESSLRCFCMTDDRVDKTLEQQENFEEVARSKD IEVLEGKPIYVDCYGNLAPLTKGGQQLVFNFYSFKENRLPFSIKIRDTSQEPCGRLSFLKEPKTTKGLPQ TAVCNLNITLPAHKKAEKADRRQSFASLALRKRYSYLTEPSMSPQSPCERTDIRMAIVADHLGLSWTELA RELNFSVDEINQIRVENPNSLISQSFMLLKKWVTRDGKNATTDALTSVLTKINRIDIVTLLEGPIFDYGN ISGTRSFADENNVFHDPVDGHPSFQVELETPMGLYCTPPNPFQQDDHFSDISSIESPFRTPSRLSDGLVP SQGNIEHPTGGPPVVTAEDTSLEDSKMDDSVTVTDPADPLDVDESQLKDLCQSECAQCWASVPGIPNDGR QAEPLRPQTRKVGMSSEQQEKGKSGPDEEVTEDKVKSLFEDIQLEEVEAEEMTEDQGQAMLNRVQRAELA MSSLAGWQNETPSGSLESPAQARRLTGGLLDRLDDSSDQARDSITSYLTGEPGKIEANGNHTAEVIPEAK AKPYFPESQNDIGKQSIKENLKPKTHGCGRTEEPVSPLTAYQKSLEETSKLVIEDAPKPCVPVGMKKMTR TTADGKARLNLQEEEGSTRSEPKQGEGYKVKTKKEIRNVEKKTH TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_170687 |
| ORF Size | 3282 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_170687.3, NP_733788.2 |
| RefSeq Size | 7554 bp |
| RefSeq ORF | 3285 bp |
| Locus ID | 11735 |
| UniProt ID | G5E8K5 |
| Gene Summary | This gene encodes a member of the ankyrin protein family. Ankyrins link integral membrane proteins to the spectrin-based cytoskeleton. Ankyrin family members share a protein structure which includes three independently folded domains: the N-terminal ankyrin repeat domain, the central spectrin-binding domain, and the C-terminal rod domain. This ankyrin functions as the major ankyrin in the kidney and may play a role in the polarized distribution of many integral membrane proteins to specific subcellular sites. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223502 | Ank3 (untagged) - Mouse ankyrin 3, epithelial (Ank3), transcript variant 6, (10ug) |
CNY 13,970.00 |
|
| MR215427 | Ank3 (Myc-DDK-tagged) - Mouse ankyrin 3, epithelial (Ank3), transcript variant 6 |
CNY 10,930.00 |
|
| MR215427L3 | Lenti ORF clone of Ank3 (Myc-DDK-tagged) - Mouse ankyrin 3, epithelial (Ank3), transcript variant 6 |
CNY 12,830.00 |
|
| MR215427L4 | Lenti ORF clone of Ank3 (mGFP-tagged) - Mouse ankyrin 3, epithelial (Ank3), transcript variant 6 |
CNY 12,830.00 |
