Slc6a18 (BC010748) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	XT2
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG209057 representing BC010748 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCACAGGCCTCAGGGATGGACCCGCTTGTGGACATTGAGGATGAAAGACCCAAGTGGGACAACAAAC TCCAGTACCTCCTGAGCTGCATCGGTTTTGCTGTGGGGCTGGGGAACATATGGAGGTTCCCCTACCTGTG CCAGACCCACGGAGGAGGGGCCTTCCTCATCCCCTATTTCATTGCCCTGGTCTTTGAGGGTATCCCGCTT TTCTACATCGAGCTTGCCATTGGCCAGCGCCTACGGAGGGGAAGCATTGGAGTGTGGAAGACCATCTCCC CTTACCTCGGTGGCGTAGGCCTGGGCTGCTTCTCAGTGTCCTTCCTGGTCAGTTTGTACTACAACACGGT TCTCCTGTGGGTCTTATGGTTCTTCCTCAACTCCTTCCAACACCCGCTGCCCTGGAGCACATGTCCGCTG GATCTCAACAGAACAGGATTTGTGCAGGAATGCCAGAGCAGTGGCACCGTGAGCTACTTCTGGTACCGGC AGACTCTGAATATCACATCTGACATCAGCAACACAGGCACTATCCAATGGAAGCTGTTCCTCTGCCTGGT GGCCTGCTGGTCAACTGTGTACCTGTGTGTCATCAGAGGCATTGAGAGCACGGGGAAGGTGATCTACTTT ACAGCCTTATTCCCTTACCTGGTGCTAACCATCTTCCTCATCAGAGGTCTTACCCTGCCTGGAGCAACAG AGGGCCTGATCTACCTGTTTACTCCCAATATGAAGACTCTTCAGAATCCACGGGTGTGGTTGGATGCAGC CACCCAGATTTTCTTCTCCCTGTCCCTGGCCTTTGGAGGGCATATTGCTTTTGCAAGCTACAACCCACCC AGGAACAATTGTGAGAAGGACGCCGTGATTATTGCCCTGGTCAACAGCATGACCTCCCTGTATGCATCCA TCGCCATCTTCTCCGTCATGGGGTTCAAGGCATCCAATGACTATGGAAGGTGCCTGGACAGAAATATCTT GAGCCTCATCAATGAGTTTGACCTTCCAGAGCTTAGCATCTCCAGGGATGAGTACCCATCTGTCCTCATG TACCTGAATGCCACTCAGACTGCGAGGGTGGCCCAACTTCCCCTGAAGACCTGCCATCTGGAAGATTTTC TGGATAAGCCCACCTGGAAGCAGATCTCCGGGGCCCGTGTTCTTGGAGAAGGCTGTGCCAGGCTGACTTC TAGAGTCTGTGAAGCCTCAGTGCTCCCAGGGGTGATCTGCTTTGCCTGTTTCCTCTCAGCCATTTGCTTC ACACTGCAGTCTGGAGGCTACTGGTTGGAGATCTTTGACAGTTTTGCAGCTTCTCTGAATTTAATCATCT TCGCCTTCATGGAAGTGGTGGGAGTCATTCACATTTATGGGATGAAACGGTTCTGTGATGACATTGAATG GATGACTGGGCGGCGGCCCGGCCTCTACTGGCAGGTGACATGGAGGGTTGTGAGCCCTATGCTGCTGTTT GGAATCTTCCTGTCCTACATTGTCCTTCTGATCCAGACACCGCCCAGTTACAAGGCCTGGAACCCCCAAT ATGAACATTTTCCCTCAAGAGAGGAGAAGTTCTACCCAGGCTGGGTGCAGGTCACCTGTGTGCTCCTGTC CTTCCTGCCCTCACTGTGGGTCCCTGGAGTTGCTCTGGCTCAGTTACTGTCCCAGTACAAACAGAGGTGG AAGGCTACGCATCTGGAAAGTGGTCTGAAGCTACAGGAGAGCAGAGGCTGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG209057 representing BC010748 Red=Cloning site Green=Tags(s) MAQASGMDPLVDIEDERPKWDNKLQYLLSCIGFAVGLGNIWRFPYLCQTHGGGAFLIPYFIALVFEGIPL FYIELAIGQRLRRGSIGVWKTISPYLGGVGLGCFSVSFLVSLYYNTVLLWVLWFFLNSFQHPLPWSTCPL DLNRTGFVQECQSSGTVSYFWYRQTLNITSDISNTGTIQWKLFLCLVACWSTVYLCVIRGIESTGKVIYF TALFPYLVLTIFLIRGLTLPGATEGLIYLFTPNMKTLQNPRVWLDAATQIFFSLSLAFGGHIAFASYNPP RNNCEKDAVIIALVNSMTSLYASIAIFSVMGFKASNDYGRCLDRNILSLINEFDLPELSISRDEYPSVLM YLNATQTARVAQLPLKTCHLEDFLDKPTWKQISGARVLGEGCARLTSRVCEASVLPGVICFACFLSAICF TLQSGGYWLEIFDSFAASLNLIIFAFMEVVGVIHIYGMKRFCDDIEWMTGRRPGLYWQVTWRVVSPMLLF GIFLSYIVLLIQTPPSYKAWNPQYEHFPSREEKFYPGWVQVTCVLLSFLPSLWVPGVALAQLLSQYKQRW KATHLESGLKLQESRGC TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC010748
ORF Size	1733 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC010748, AAH10748
RefSeq Size	1904 bp
RefSeq ORF	1733 bp
Locus ID	22598
Gene Summary	Functions as a sodium and chloride-dependent neutral amino acid transporter in kidneys (PubMed:26240152, PubMed:19478081). Required CLTRN for cell surface expression and for its amino acid transporter activity (PubMed:26240152).[UniProtKB/Swiss-Prot Function]

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